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Development of twenty sequence-tagged microsatellites for the Atlantic cod (Gadus morhua L.)

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Abstract

Fifty-four primer pairs were designed for expressed sequence tag (EST) sequences containing perfect di- and tri-nucleotide motifs and characterised in 96 unrelated fish. Twenty markers were successfully amplified with number of alleles from 2 to 10 per locus and observed and expected heterozygosity ranging from 0.01 to 0.56 and 0.03 to 0.70, respectively. Loci Gmo-C213, Gmo-C246 and Gmo-C247 deviated from Hardy–Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C213 and Gmo-C222, Gmo-C233 and Gmo-C229, C223 and Gmo-C236 and C229 and Gmo-C236. The gene identity was determined at 10 of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.

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Acknowledgements

The present study was supported by the National Programme for Research in Functional Genomics in Norway (FUGE) within the Research Council of Norway (GenoFish). We thank Dr. Marie Cooper for reading of the manuscript and making helpful suggestions.

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Authors and Affiliations

  1. Norwegian Institute of Fisheries and Aquaculture Research, Muninbakken 9-13, Breivika, P.O. Box 6122, Tromso, 9291, Norway

    Madjid Delghandi, Mette Serine Wesmajervi & Saskia Mennen

  2. Department of Marine Science and Fisheries, College of Agricultural and Marine Sciences, Sultan Qaboos University, P.O. Box 34, Al-khoud 123, Muscat, Sultanate of Oman

    Madjid Delghandi

  3. University of Bergen, Institute of Biology, HIB - Thormøhlensgt. 55, Bergen, 5020, Norway

    Frank Nilsen

Authors
  1. Madjid Delghandi
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  2. Mette Serine Wesmajervi
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  3. Saskia Mennen
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  4. Frank Nilsen
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Correspondence to Madjid Delghandi.

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Delghandi, M., Wesmajervi, M.S., Mennen, S. et al. Development of twenty sequence-tagged microsatellites for the Atlantic cod (Gadus morhua L.). Conserv Genet 9, 1395–1398 (2008). https://doi.org/10.1007/s10592-008-9507-3

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  • DOI: https://doi.org/10.1007/s10592-008-9507-3

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