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Ancient DNA reveals genotypic relationships among Oregon populations of the sea otter (Enhydra lutris)

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Abstract

The sea otter has experienced a dramatic population decline caused by intense human harvesting, followed by a century of recovery including relocation efforts to reestablish the species across its former range in the eastern Pacific. Although the otter was historically present along the coast in Oregon, there are currently no populations in this region and reintroduction efforts have failed. We examined the mtDNA genotypes of 16 pre-harvest otter samples from two Oregon locations in an attempt to determine the best genotypic match with extant populations. Our amplifications of a 222 base-pair portion of the control region from otters ranging in age from approximately 175–2000 years revealed four genotypes. The genotypic composition of pre-harvest otter populations appears to match best with those of contemporary populations from California and not from Alaska, where reintroduction stocks are typically derived.

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References

  • Avise JC, Arnold J, Ball RM, Bermingham E, Lamb T, Neigel J, Reeb C, Saunders N (1987) Intraspecific phylogeography: The mitochondrial DNA bridge between population genetics and systematics. Annu Rev Ecol Syst 18:489–522

    Google Scholar 

  • Bandelt H-J, Forster P, Röhl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16:37–48

    PubMed  CAS  Google Scholar 

  • Bodkin JL, Ballachey BE, Cronin MA, Scribner KT (1999) Population demographics and genetic diversity in remnant and translocated populations of sea otters. Conserv Biol 13:1378–1385

    Article  Google Scholar 

  • Butler VL, Bowers NJ (1998) Ancient DNA from salmon bone: a preliminary study. Anc Biomol 2:17–26

    CAS  Google Scholar 

  • Clark L (1991) Archaeology of seal rock (35Lnc14). In: Lyman RL (ed) The prehistory of the oregon coast: The effects of excavation strategies and assemblage size on archaeological inquiry. Academic Press, New York, pp 175–240

    Google Scholar 

  • Cronin MA, Bodkin J, Ballachey B, Estes J, Patton JC (1996) Mitochondrial-DNA variation among subspecies and populations of sea otters (Enhydra lutris). J Mammal 77:546–557

    Article  Google Scholar 

  • Davis J, Lidicker WZ Jr. (1975) The taxonomic status of the southern sea otter. Proc Calif Acad Sci 40:429–437

    Google Scholar 

  • Gilbert MTP, Bandelt H-J, Hofreiter M, Barnes I (2005) Assessing ancient DNA studies. Trends Ecol Evol 20:541–544

    Article  PubMed  Google Scholar 

  • Gorbics CS, Bodkin JL (2001) Stock structure of sea otters (Enhydra lytris kenyoni) in Alaska. Mar Mamm Sci 17:632–647

    Article  Google Scholar 

  • Hall RL (1995) People of the Coquille Estuary. Words and Pictures Unlimited, Corvallis, OR

    Google Scholar 

  • Jameson RJ, Kenyon KW, Johnson AM, Wight HW (1982) History and status of translocated sea otter populations in North America. Wildl Soc Bull 10:100–107

    Google Scholar 

  • Kenyon KW (1969) The sea otter in the eastern Pacific Ocean. North Am Fauna 68:1–352

    Article  Google Scholar 

  • Larson S, Jameson R, Bodkin J, Staedler M, Bentzen P (2002a) Microsatellite DNA and mitochondrial DNA variation in remnant and translocated sea otter (Enhydra lutris) populations. J Mammal 83:893–906

    Article  Google Scholar 

  • Larson S, Jameson R, Etnier M, Flemings M, Bentzen P (2002b) Loss of genetic diversity in sea otters (Enhydra lutris) associated with the fur trade of the 18th and 19th centuries. Mol Ecol 11:1899–1903

    Article  PubMed  CAS  Google Scholar 

  • Lyman RL (1988) Zoogeography of Oregon coast marine mammals: the last 3,000 years. Mar Mamm Sci 4:247–264

    Article  Google Scholar 

  • Lyman RL (1996) Applied zooarchaeology: the relevance of faunal analysis to wildlife management. World Archaeol 28:110–125

    Article  Google Scholar 

  • O’Brien SJ, Mayr E (1991) Bureaucratic mischief: recognizing endangered species and subspecies. Science 251:1187–1188

    Article  PubMed  Google Scholar 

  • Riedman ML, Estes JA (1990) The sea otter (Enhydra lutris): behavior, ecology, and natural history. U.S. Fish & Wildlife Service Biology Report 90,126 pp

  • Rotterman LM (1992) Patterns of genetic variability in sea otters after severe population subdivision and reduction. Ph.D. dissertation, University of Minnesota, Minneapolis, 227 pp

  • Rozas J, Rozas R (1999) DnaSP version 3: an integrated program for moleulcar population genetic and molecular evolution analysis. Bioinformatics 15:174–175

    Article  PubMed  CAS  Google Scholar 

  • Scribner KT, Bodkin J, Ballachey B, Fain SR, Cronin MA, Sanchez M (1997) Population genetic studies of the sea otter (Enhydra lutris): a review and interpretation of available data. Mol Gen Mar Mamm 3:197–208

    Google Scholar 

  • Tul’skaya OL, Derenko MV, Malyarchuk BA (1999) Low level of mitochondrial DNA variation in sea otter populations from Kamchatka and Komandor Islands. Russ J Genetics 35:17–21

    Google Scholar 

  • Weber DS, Stewart BS, Garza JC, Lehman N (2000) An empirical genetic assessment of the severity of the northern elephant seal population bottleneck. Curr Biol 10:1287–1290

    Article  PubMed  CAS  Google Scholar 

  • Weber DS, Stewart BS, Lehman N (2004) Genetic consequences of a severe population bottleneck in the Guadalupe fur seal (Arctocephalus townsendi). J Hered 95:144–153

    Article  PubMed  CAS  Google Scholar 

  • Wilson DE, Bogan MA, Brownell RL Jr, Burdin AM, Maminov MK (1991) Geographic variation in the sea otters, Enhydra lutris. J Mammal 72:22–36

    Article  Google Scholar 

  • Yang DY, Eng B, Waye JS, Dudar JC, Saunders SR (1998) Improved DNA extractions from ancient bones using silica-based spin columns. Am J Phys Anthropol 105:539–543

    Article  PubMed  CAS  Google Scholar 

  • Yu W, Rusterholtz KJ, Krummel AT, Lehman N (2006) Detection of high levels of recombination generated during PCR amplification of RNA templates. BioTechniques 40:499–507

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

We thank Rebekah Hull and Klaus Koepfli for technical assistance, and the Oregon Sea Grant program and EcoTrust for funding. We also acknowledge the support of the Confederated Tribes of Siletz Indians and the Coquille Indian Tribe.

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Correspondence to Niles Lehman.

Appendix

Appendix

  Sea otters genotyped in this study

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Valentine, K., Duffield, D.A., Patrick, L.E. et al. Ancient DNA reveals genotypic relationships among Oregon populations of the sea otter (Enhydra lutris). Conserv Genet 9, 933–938 (2008). https://doi.org/10.1007/s10592-007-9422-z

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  • DOI: https://doi.org/10.1007/s10592-007-9422-z

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