Skip to main content
SpringerLink
Log in

Development and characterization of microsatellite markers for a mangrove tree species Sonneratia caseolaris (L.) Engler (Lythraceae sensu lato)

  • Technical Note
  • Published:
Conservation Genetics Aims and scope Submit manuscript

Abstract

Sonneratia caseolaris is a typical non-viviparous mangrove species and a key component of mangrove community in the Indo-West Pacific region. Here we isolated nine microsatellite simple sequence repeat (SSR) loci from the genome of S. caseolaris. Our isolated loci provided SSR markers with polymorphism of 2–6 alleles per locus. The expected and observed heterozygosities ranged from 0.242 to 0.745 and from 0.083 to 0.417, respectively. Cross-species amplification in S. alba and S. ovata showed that a subset of these markers holds promise for these congeneric species. These polymorphic SSR markers would be useful tools for population genetics studies on S. caseolaris as well as other congeneric species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Castillo-Cardenas MF, Toro-Perea N (2007) Development and characterization of the first microsatellite markers in the mangrove species Pelliciera rhizophorae Triana & Planchon. Mol Ecol Notes (in press). DOI 10.1111/j.1471-8286.2007.01841.x

  • Chen G-Q, Li L-F, Hao G, Shi S-H, Ge X-J (2007) Characterization of seven genomic and one dbEST-derived microsatellite loci in the river mangrove Aegiceras corniculatum (Myrsinaceae). Conserv Genet (in press). DOI 10.1007/s10592-007-9327-x

  • Clarke KR, Gorley RN (2001) PRIMER v5: user manual/tutorial. PRIMER-E Ltd., Plymouth, UK

    Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • FAO (1994) Mangrove forest management guidelines. FAO, Rome

    Google Scholar 

  • Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html

  • Islam MS, Lian CL, Kameyama N, Wu B, Hogetsu T (2004) Development of microsatellite markers in Rhizophora stylosa using a dual-suppression-polymerase chain reaction technique. Mol Ecol Notes 4:110–112

    Article  CAS  Google Scholar 

  • Islam MS, Lian CL, Kameyama N, Wu B, Hogetsu T (2006a) Development and characterization of ten new microsatellite markers in a mangrove tree species Bruguiera gymnorrhiza (L.). Mol Ecol Notes 6:30–32

    Article  CAS  Google Scholar 

  • Islam MS, Tao JM, Geng QF, Lian CL, Hogetsu T (2006b) Isolation and characterization of eight compound microsatellite markers in a mangrove tree Kandelia candel (L.) Druce. Mol Ecol Notes 6:1111–1113

    Article  CAS  Google Scholar 

  • Nettel A, Rafii F, Dodd RS (2005) Characterization of microsatellite markers for the mangrove tree Avicennia germinans L. (Avicenniaceae). Mol Ecol Notes 5:103–105

    Article  CAS  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (30500049) to S. Shi and the Young Teacher Foundation of Sun Yat-Sen University (2006-33000-1131357) to R. Zhou.

Author information

Authors and Affiliations

  1. State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, Sun Yat-Sen University, Guangzhou, 510275, P. R. China

    Tao Chen, Renchao Zhou, Xue-Jun Ge & Suhua Shi

  2. School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, Guangdong, P. R. China

    Renchao Zhou

Authors
  1. Tao Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Renchao Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xue-Jun Ge
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Suhua Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Renchao Zhou.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, T., Zhou, R., Ge, XJ. et al. Development and characterization of microsatellite markers for a mangrove tree species Sonneratia caseolaris (L.) Engler (Lythraceae sensu lato). Conserv Genet 9, 957–959 (2008). https://doi.org/10.1007/s10592-007-9404-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10592-007-9404-1

Keywords

Search

Navigation