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Development of ten microsatellite loci for Gentiana crassicaulis (Gentianaceae)

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Abstract

Genetiana crassicaulis is one of famous Chinese medicinal plant. The over-collection for its root has caused its dramatic reduction. In order to devise adequate conservation and management strategies for this species, it is important to characterize its genetic diversity and understand its population structure. Here, 10 polymorphic microsatellite markers have been developed. AC/TG microsatellite was enriched by combining biotin capture method. Polymorphism of each locus was assessed in 30 individuals from six populations. The number of alleles ranged from 2 to 9 and the expected heterozygosity ranged from 0.32 to 0.78.

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Acknowledgements

This work was financially supported by the “Talented People Project of the Sun Yat-Sen University”.

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Authors and Affiliations

  1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Xingang W Road 125, Guangzhou, Guangdong, 510275, China

    Yong Li, Lin-Feng Li & Xue-Jun Ge

  2. South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, China

    Guo-Qing Chen

Authors
  1. Yong Li
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  2. Lin-Feng Li
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  3. Guo-Qing Chen
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  4. Xue-Jun Ge
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Correspondence to Xue-Jun Ge.

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Li, Y., Li, LF., Chen, GQ. et al. Development of ten microsatellite loci for Gentiana crassicaulis (Gentianaceae). Conserv Genet 8, 1499–1501 (2007). https://doi.org/10.1007/s10592-007-9313-3

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  • DOI: https://doi.org/10.1007/s10592-007-9313-3

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