Abstract
Rare minnow (Gobiocypris rarus) is an endangered small fish endemic to upper reach of the Yangtze River. From a (GT)n enriched genomic library, 32 microsatellites were isolated and characterized. Nineteen of these loci were polymorphic in a test population with alleles ranging from 2–7, and observed and expected heterozygosities from zero to 0.8438, and 0.2679 to 0.8264, respectively. In the cross-species amplifications, 13 out of 19 polymorphic loci were found to be also polymorphic in at least one of the 7 closely related species of the subfamily Gobioninae. These polymorphic microsatellite loci should provide sufficient level of genetic diversity to evaluate the fine-scale population structure in rare minnow and its closely related species for the conservation purpose.
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Acknowledgements
The authors thank Drs. J. Zhou, Z. Wang, B. Zhu and Ms C.You for their technical assistance. This study was supported by NSFC (30370225, 30271011) and “973” Project (2004CB117405).
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Liao, X., Wang, D., Yu, X. et al. Characterization of novel microsatellite loci in rare minnow (Gobiocypris rarus) and amplification in closely related species in Gobioninae. Conserv Genet 8, 1003–1007 (2007). https://doi.org/10.1007/s10592-006-9222-x
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DOI: https://doi.org/10.1007/s10592-006-9222-x