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Contrasting levels of genetic differentiation among populations of wolverines (Gulo gulo) from northern Canada revealed by nuclear and mitochondrial loci

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Abstract

Habitat loss, fragmentation, overharvest, and other anthropogenic factors have resulted in population and distribution declines for North American wolverines (Gulo gulo). Currently, wolverines east of the Hudson Bay are endangered and possibly extinct, whereas the status of wolverines throughout the remaining Holarctic is vulnerable. Three previous studies using nuclear loci have detected little to no significant structuring among wolverines sampled across northern Canada. Based on these results it has been suggested that wolverines in northern Canada represent a single, panmictic population. However, as has been shown in numerous studies, in cases of female site fidelity, it is possible to have demographically autonomous populations even with male-biased gene flow. To better assess the genetic structure of wolverines in northern Canada, we examined nine microsatellite loci and DNA sequence variation from a 200 bp fragment of the mitochondrial (mtDNA) control region for 270 wolverines from nine collecting areas representing three regions of northern Canada. In agreement with previous studies, microsatellite analyses revealed a lack of significant population substructure (F ST=0.0004). However, analysis of molecular variance, comparisons of pairwise F ST values and nested-clade analysis of the mtDNA data revealed considerable genetic structuring among samples of wolverines from these three regions of northern Canada. These mitochondrial data provide evidence that wolverines in Canada are genetically structured due to female philopatry. The contrasting patterns of genetic differentiation based on nuclear and mitochondrial data highlight the importance of examining both nuclear and mitochondrial loci when attempting to elucidate patterns of genetic structure.

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References

  • Avise JC (1995) Mitochondrial DNA polymorphism and a connection between genetics and demography of relevance to conservation. Conserv. Biol., 9, 686–690.

    Google Scholar 

  • Avise JC (2000) Phylogeography:the History and Formation of Species. Harvard University Press, Cambridge.

    Google Scholar 

  • Banci V (1994) Wolverine. In:The Scientific Basis for Conserving Forest Carnivores:American Marten, Fisher, Lynx, and Wolverine in western United States (eds. Ruggiero LF, Aubry KB, Buskirk SW, Lyon LJ, Zielinski WJ), pp. 99–27. United States Forest Service General Technical Report RM-254. Fort Collins.

  • Cegelski CC, Waits LP, Anderson J (2003) Assessing population structure and gene. ow in Montana wolverines. (Gulo gulo )using assignment-based approaches. Mol. Ecol. 12, 2907–2918.

    Google Scholar 

  • Clement M, Posada D, Crandall KA (2000) TCS:a computer program to estimate gene genealogies. Mol. Ecol. 9, 1657–1659.

    Google Scholar 

  • Copeland JP (1996) Biology of the Wolverine in Central Idaho. MS thesis, University of Idaho, Moscow, Idaho.

    Google Scholar 

  • COSEWIC (2001). Canadian Species at risk:Committee on the Status of Endangered Wildlife in Canada. 18 pp. Ottawa.

  • Crandall KA, Templeton AR (1993) Empirical tests of some predictions from coalescent theory with applications to intraspecific phylogeny construction. Genetics 134, 959–969.

    Google Scholar 

  • Davis CS, Strobeck C (1998) Isolation, variability, and cross-species ampli. cation of polymorphic microsatellite loci in the family Mustelidae. Mol. Ecol. 7, 1776–1778.

    Google Scholar 

  • Duffy AJ, Landa A, O'Connell M, Stratton C, Wright JM (1998) Four polymorphic microsatellites in wolverines. Gulo gulo. Anim. Genet. 29, 63–72.

    Google Scholar 

  • Gardner CL (1985) The Ecology of Wolverines in Southcentral Alaska. MS thesis, University of Alaska.

  • Gardner CL, Ballard WB, Jessup RH (1986). Long distance movement by an adult wolverine. J. Mamm. 67, 603.

    Google Scholar 

  • Hash HS (1987) Wolverine. In:Wild Furbearer Management and Conservation in North America (eds. Novak M, Baker JA, Obbard ME, Malloch B). pp. 575–585. Ontario Ministry of Natural Resources, Ontario.

    Google Scholar 

  • Kyle CJ, Strobeck C (2001) Genetic structure of North American wolverine (Gulo gulo )populations. Mol. Ecol. 10, 337–347.

    Google Scholar 

  • Kyle CJ, Strobeck C (2002) Connectivity of peripheral and core populations of North American wolverines. J. Mamm. 83, 1141–1150.

    Google Scholar 

  • Longmire JL, Maltbie M, Baker RJ (1997) Use of lysis bu. er in DNA isolation and its implications for museum collections. Occas. Papers, the Mus., Texas Tech Univ. 163, 1–3.

    Google Scholar 

  • Maddison DR, Maddison WP (2000) MacClade:Analysis of Phylogeny and Character Evolution. Version 4. 0. Sinauer Associates, Inc., Sunderland, MA.

    Google Scholar 

  • Magoun AJ (1985) Population Characteristics, Ecology, and Management of Wolverines in Northwestern Alaska. Ph. D. thesis, University of Alaska.

  • Manel S, Berthier P, Luikary G (2002) Detecting wildlife poaching:identifying the origin of individuals with Bayesian assignment tests and multilocus genotypes. Conserv. Biol. 16, 650–659.

    Google Scholar 

  • Nowak RM (1991) Walker 's Mammals of the World. The John Hopkins Press, Baltimore.

    Google Scholar 

  • O 'Connell M, Wright JM, Faird A (1996) Development of PCR primers for nine polymorphic American mink, Mustela vison microsatellite loci. Mol. Ecol. 5, 311–312.

    Google Scholar 

  • Oxford Molecular Group PLC 1998. AssemblyLIGN. Versison 1. 0. 9. Oxford Molecular Group PLC, Oxford.

    Google Scholar 

  • Posada D, Crandall KA, Templeton AR (2000) GeoDis:a program for the cladistic nested analysis of the geographical distribution of genetic haplotypes. Mol. Ecol. 9, 487–488.

    Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155, 945–959.

    Google Scholar 

  • Rueness EK, Stenseth NC, O 'Donoghue M, Boutin S, Ellegren H, Jakobsen KS (2003) Ecological and genetic spatial structuring in the Canadian lynx. Nature 425, 69–72.

    Google Scholar 

  • Schneider S, Roessli D, Excoffier L (2000) Arlequin:a Software for Population Genetic Analysis. Version 2. 000. Genetics and Biometry Laboratory, University of Geneva, Switzerland.

  • Slatkin M (1985) Gene flow in natural populations. Ann. Rev. Ecol. Syst. 16, 393–430.

    Google Scholar 

  • Swofford DL (2001) PAUP*:Phylogenetic Analysis Using Parsimony (*and other methods). Version 4. 0 b10. Sinauer Associates, Inc., Publishers, Sunderland, MA.

    Google Scholar 

  • Tamur K, Nei M(1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 10, 512–526.

    Google Scholar 

  • Templeton AR (1998) Nested clade analyses of phylogenetic data:testing hypotheses about gene flow and population history. Mol. Ecol. 7, 381–397.

    Google Scholar 

  • Templeton AR, Sing CF (1993) A cladistic analysis of pheno-typic associations with haplotypes inferred from restriction endonuclease mapping IV:nested analyses with cladogram uncertainty and recombination. Genetics 134, 659–669.

    Google Scholar 

  • Templeton AR, Boerwinkle E, Sing CF (1987) A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping. I. Basic theory and an analysis of alcohol dehydrogenase activity in Drosophila. Genetics 117, 343–351.

    Google Scholar 

  • Templeton AR, Routman E, Phillips CA (1995) Separating population structure from population history:a cladistic analysis of the geographical distribution of mitochondrial DNA haplotypes in the tiger salamander, Ambystoma tigrinum. Genetics 140, 767–782.

    Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic. Acids Res. 25, 4876–4882.

    Google Scholar 

  • Vangen KM, Persson J, Landa A, Anderson R, Segerstro ¨m P (2001) Characteristics of dispersal in wolverines. Can. J. Zool. 79, 1641–1649.

    Google Scholar 

  • Walker CW, Vila C, Landa A, Linden M, Ellegren H (2001) Genetic variation and population structure in Scandinavian wolverine (Gulo gulo )populations. Mol. Ecol. 10, 53–63.

    Google Scholar 

  • Wilson DE (1982) Wolverine Gulo gulo. In:Wild Mammals of North America. Biology, Management and Economics (eds. Chapman JA, Feldhamer GA). pp. 644–652. The John Hopkins University Press, Baltimore.

    Google Scholar 

  • Wilson GM, Van Den Bussche RA, Kennedy PK, Gunne A, Poole K (2000) Genetic variability of wolverines (Gulo gulo ) from the Northwest Territories, Canada:conservation implications. J. Mamm. 81, 186–196.

    Google Scholar 

  • Wright S (1965) The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution 19, 395–420.

    Google Scholar 

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Chappell, D.E., Van Den Bussche, R.A., Krizan, J. et al. Contrasting levels of genetic differentiation among populations of wolverines (Gulo gulo) from northern Canada revealed by nuclear and mitochondrial loci. Conservation Genetics 5, 759–767 (2004). https://doi.org/10.1007/s10592-004-1976-4

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  • DOI: https://doi.org/10.1007/s10592-004-1976-4

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