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Comparative cytogenomics of poultry: mapping of single gene and repeat loci in the Japanese quail (Coturnix japonica)

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Abstract

Well-characterized molecular and cytogenetic maps are yet to be established in Japanese quail (Coturnix japonica). The aim of the current study was to cytogenetically map and determine linkage of specific genes and gene complexes in Japanese quail through the use of chicken (Gallus gallus) and turkey (Meleagris gallopavo) genomic DNA probes and conduct a comparative study among the three genomes. Chicken and turkey clones were used as probes on mitotic metaphase and meiotic pachytene stage chromosomes of the three species for the purpose of high-resolution fluorescence in situ hybridization (FISH). The genes and complexes studied included telomerase RNA (TR), telomerase reverse transcriptase (TERT), 5S rDNA, 18S-5.8S-28S rDNA (i.e., nucleolus organizer region (NOR)), and the major histocompatibility complex (MHC). The telomeric profile of Japanese quail was investigated through the use of FISH with a TTAGGG-PNA probe. A range of telomeric array sizes were confirmed as found for the other poultry species. Three NOR loci were identified in Japanese quail, and single loci each for TR, TERT, 5S rDNA and the MHC-B. The MHC-B and one NOR locus were linked on a microchromosome in Japanese quail. We confirmed physical linkage of 5S rDNA and the TR gene on an intermediate-sized chromosome in quail, similar to both chicken and turkey. TERT localized to CJA 2 in quail and the orthologous chromosome region in chicken (GGA 2) and in turkey (MGA 3). The cytogenetic profile of Japanese quail was further developed by this study and synteny was identified among the three poultry species.

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Abbreviations

BAC:

Bacterial artificial chromosome

CE:

Chicken embryo

CEF:

Chicken embryo fibroblast

CJA:

Coturnix japonica

DAPI:

4′,6-Diamidino-2-phenylindole

E:

Embryonic day or days of embryogenesis

ETS:

External transcribed spacer

FISH:

Fluorescence in situ hybridization

GGA:

Gallus gallus

MGA:

Meleagris gallopavo

MHC:

Major histocompatibility complex

MY:

Million years

NOR:

Nucleolus organizer region

PNA:

Peptide nucleic acid

rDNA:

Ribosomal DNA

TEF:

Turkey embryo fibroblast

TERT:

Telomerase reverse transcriptase

TR:

Telomerase RNA

UCD:

University of California, Davis

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Acknowledgements

The authors greatly appreciate the research contributions of Andrew Jiang, Thomas H. O’Hare, and Elizabeth A. Robb. Research was funded by United States Department of Agriculture-National Institute of Food and Agriculture multistate and nation projects: National Research Support Program-8 (CA-D*-ASC-7233-RR), NC-1170 (CA-D*-ASC-6414-RR), and NE-1034 (CA-D*-ASC-7281-RR). We appreciate the support of the California Agricultural Experiment Station and the Department of Animal Science and College of Agricultural and Environmental Sciences at the University of California, Davis.

Ethical standards

The authors declare that all experiments performed in this study comply with the current laws of the USA. All institutional and national guidelines for the care and use of laboratory animals were followed.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Animal Science, University of California, One Shields Ave, Davis, CA, 95616, USA

    Marla C. McPherson, Charmaine M. Robinson, Lida P. Gehlen & Mary E. Delany

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  1. Marla C. McPherson
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  2. Charmaine M. Robinson
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  3. Lida P. Gehlen
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  4. Mary E. Delany
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Correspondence to Mary E. Delany.

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Responsible Editors: Darren K. Griffin and Beth A. Sullivan.

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McPherson, M.C., Robinson, C.M., Gehlen, L.P. et al. Comparative cytogenomics of poultry: mapping of single gene and repeat loci in the Japanese quail (Coturnix japonica). Chromosome Res 22, 71–83 (2014). https://doi.org/10.1007/s10577-014-9411-2

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