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Quantitative variation of LINE-1 sequences in five species and three subspecies of the subgenus Mus and in five Robertsonian races of Mus musculus domesticus

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Abstract

The quantitative variation of a conserved region of the LINE-1 ORF2 sequence was determined in eight species and subspecies of the subgenus Mus (M. m. domesticus, M. m. musculus, M. m. castaneus, M. spicilegus, M. spretus, M. cervicolor, M. cookii, M. caroli) and five Robertsonian races of M. m. domesticus. No differences in LINE-1 ORF2 content were found between all acrocentric or Robertsonian chromosome races, whereas the quantitative variation of the LINE-1 ORF2 sequences detected among the eight taxa partly matches with the clades into which the subgenus is divided. An accumulation of LINE-1 ORF2 elements likely occurred during the evolution of the subgenus. Within the Asiatic clade, M. cervicolor, cookii, and caroli show a low quantity of LINE-1 sequences, also detected within the Palearctic clade in M. m. castaneus and M. spretus, representing perhaps the ancestral condition within the subgenus. On the other hand, M. m. domesticus, M. m. musculus and M. spicilegus showed a high content of LINE-1 ORF2 sequences. Comparison between the chromosomal hybridization pattern of M. m. domesticus, which possesses the highest content, and M. spicilegus did not show any difference in the LINE-1 ORF2 distribution, suggesting that the quantitative variation of this sequence family did not involve chromosome restructuring or a preferential chromosome accumulation, during the evolution of M. m. domesticus.

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Abbreviations

3′UTR:

3′ untranslated region

5′UTR:

5′ untranslated region

ANOVA:

analysis of variance

bp:

base pair

BSA:

bovine serum albumin

DAPI:

4′,6-diamidino-2-phenylindole

dCTP:

deoxycytidine triphosphate

DNA:

deoxyribonucleic acid

dNTP:

deoxyribonucleotide

FISH:

fluorescence in-situ hybridization

kb:

kilobase

LINE-1:

long interspersed nuclear element-1

LTR:

long terminal repeat

MITE:

miniature inverted-repeat transposable element

mtDNA:

mitochondrial deoxyribonucleic acid

NIH:

National Institutes of Health

ORF1:

open reading frame 1

ORF2:

open reading frame 2

PBS:

phosphate-buffered saline

PCR:

polymerase chain reaction

Rb:

Robertsonian

RNA:

ribonucleic acid

SDS:

sodium dodecyl sulfate

SNPs:

single-nucleotide polymorphism

SSC:

sodium chloride and sodium citrate

TEs:

transposable elements

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Acknowledgements

This work was supported by PRIN-COFIN 2005, Cariplo Foundation and FIRB 2005 (Project N.RBIP06FH7J) (Italy).

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Authors and Affiliations

  1. Dipartimento di Biologia Animale, Università degli Studi di Pavia, Piazza Botta, 9-10, 27100, Pavia, Italy

    Paola Rebuzzini & Silvia Garagna

  2. Dipartimento di Biologia Animale e dell’Uomo, Università ‘La Sapienza’ di Roma, Via Borelli 50, 00161, Rome, Italy

    Riccardo Castiglia & Ernesto Capanna

  3. Dipartimento di Genetica e Microbiologia ‘Adriano Buzzati-Traverso’, Università degli Studi di Pavia, Via Ferrata 1, 27100, Pavia, Italy

    Solomon G. Nergadze

  4. Laboratory of Zoology, Section of Animal Biology, Department of Biology, University of Patras, GR-26500, Patras, Greece

    George Mitsainas

  5. Biodiversity Research Group, Department of Zoology, Faculty of Science, Charles University, Vinicna 7, 44, Prague 2, Czech Republic

    Pavel Munclinger

  6. Sezione di Istologia ed Embriologia, Dipartimento di Medicina Sperimentale, Università degli Studi di Parma, Via Volturno 39, 43100, Parma, Italy

    Maurizio Zuccotti

  7. Fondazione I.R.C.C.S. Policlinico San Matteo, Viale Golgi 19, 27100, Pavia, Italy

    Carlo Alberto Redi

Authors
  1. Paola Rebuzzini
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  2. Riccardo Castiglia
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  3. Solomon G. Nergadze
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  4. George Mitsainas
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  6. Maurizio Zuccotti
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  7. Ernesto Capanna
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  8. Carlo Alberto Redi
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  9. Silvia Garagna
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Corresponding author

Correspondence to Silvia Garagna.

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Responsible Editor: Walther Traut.

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Rebuzzini, P., Castiglia, R., Nergadze, S.G. et al. Quantitative variation of LINE-1 sequences in five species and three subspecies of the subgenus Mus and in five Robertsonian races of Mus musculus domesticus . Chromosome Res 17, 65–76 (2009). https://doi.org/10.1007/s10577-008-9004-z

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  • DOI: https://doi.org/10.1007/s10577-008-9004-z

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