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Neuroprotective Effects of 2-Cyclopropylimino-3-Methyl-1,3-Thiazoline Hydrochloride Against Oxidative Stress

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Abstract

Oxidative stress, glutamate excitotoxicity, and inflammation are the important pathological mechanisms in neurodegenerative diseases. Recently, we reported that 2-cyclopropylimino-3-methyl-1,3-thiazoline hydrochloride protects rat glial cells against glutamate-induced excitotoxicity. In this study, we report the effects of 2-cyclopropylimino-3-methyl-1,3-thiazoline hydrochloride on primary cultured cortical astrocytes after exposure to hydrogen peroxide (H2O2). Pretreatment of cells with 2-cyclopropylimino-3-methyl-1,3-thiazoline hydrochloride prior to H2O2 exposure attenuated the H2O2-induced reductions in cell survival and superoxide dismutase, catalase, glutathione, and glutathione peroxidase activities. It also reduced H2O2-induced increases in reactive oxygen species levels, malondialdehyde content, and production of nitric oxide. These effects were all concentration-dependent. Our results suggest that 2-cyclopropylimino-3-methyl-1,3-thiazoline hydrochloride protects against oxidative stress.

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Acknowledgments

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0073270 & 2010-0016830), by the Asan Institute for Life Sciences (2009-010), and by a Student Research Grant of the University of Ulsan College of Medicine, Seoul, Korea.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul, 138-736, Korea

    Hanwook Kim, Hyo Jeong Son, Seung Cheol Ha, Eun-A Kim & Sung-Woo Cho

  2. Department of Biomedical Laboratory Science, Yonsei University, Wonju, 222-701, Korea

    Eun-A Kim & Tae Ue Kim

  3. Department of Biomedical Science, Research Institute for Bioscience and Biotechnology, and Medical & Bio-material Research Center, Hallym University, Chunchon, 200-702, Korea

    Soo Young Choi

  4. Department of Molecular Cell Biology, Center for Molecular Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, 440-746, Korea

    Jee-Yin Ahn

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  1. Hanwook Kim
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Correspondence to Sung-Woo Cho.

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Hanwook Kim, Hyo Jeong Son, and Seung Cheol Ha contributed equally to this study.

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Kim, H., Son, H.J., Ha, S.C. et al. Neuroprotective Effects of 2-Cyclopropylimino-3-Methyl-1,3-Thiazoline Hydrochloride Against Oxidative Stress. Cell Mol Neurobiol 31, 979–984 (2011). https://doi.org/10.1007/s10571-011-9713-2

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  • DOI: https://doi.org/10.1007/s10571-011-9713-2

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