Abstract
Bamboo has excellent mechanical properties compared to wood and other plant materials, due to its multilayered structure and polytropic microfibril angle (MFA). The micro/nano scale structure and MFA of fibers, parenchyma cells, and vessels from 4-year-old Moso bamboo (Phyllostachys Heterocycla Var. Pubescens) were investigated by a novel LC-PolScope imaging system and transmission electron microscopy. At the nanoscale, the numbers of layers and accurate MFA for each layer especially thin layers could be obtained quickly using this novel LC-PolScope imaging system. Based on the differences of structure and shape, fibers and parenchyma cells in the vascular bundle were divided into FI, II, III and PI, II cells, respectively. The former class of FI, II, III included 2, 6–8, and 6–8 secondary cell wall layers in turn. The latter class exhibited 9 secondary cell wall layers, with a maximum of 16 layers. To our knowledge, this is the first report of accurate MFA measurement based on the differences of structure and shape for every layer of single fibers, parenchyma cells and vessels in the vascular bundle. For all three cell types, the results also showed that the MFA of sub-layers in secondary walls followed the same changing law: alternating smaller and then bigger MFA. This structural form may be the consequence of natural selection and optimization indicating the long-term mechanical adaptation of bamboo.
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Abe H, Funada R, Ohtani J, Fukazawa K (1997) Changes in the arrangement of cellulose microfibrils associated with the cessation of cell expansion in tracheids. Trees-Struct Funct 11:328–332. doi:10.1007/s004680050092
Abraham Y, Elbaum R (2013) Quantification of microfibril angle in secondary cell walls at subcellular resolution by means of polarized light microscopy. New Phytol 197:1012–1019. doi:10.1111/nph.12070
Ahvenainen P, Dixon PG, Kallonen A, Suhonen H, Gibson LJ, Svedström K (2017) Spatially-localized bench-top X-ray scattering reveals tissue-specific microfibril orientation in Moso bamboo. Plant Methods 13(1):5. doi:10.1186/s13007-016-0155-1
An X (2016) Microfibril orientations and ultrastructure of fibers wall from Moso bamboo. Ph.D. dissertation, Chinese Academy of Forestry, Beijing, China
Burgert I, Keckes J, Frühmann K, Fratzl P, Tschegg SE (2002) A comparison of two techniques for wood fibre isolation-evaluation by tensile tests on single fibres with different microfibril angle. Plant Biol 4(01):9–12. doi:10.1055/s-2002-20430
Cave I (1966) Theory of X-ray measurement of microfibril angle in wood. For Prod J 16:37–43
Cave I (1968) The anisotropic elasticity of the plant cell wall. Wood Sci Technol 2(4):268–278
Cha MY, Lee KH, Kim YS (2014) Micromorphological and chemical aspects of archaeological bamboos under long-term waterlogged condition. Int Biodeterior Biodegradation 86:115–121. doi:10.1016/j.ibiod.2013.08.008
Chen H (2014) Study on the structural characteristics of bamboo cell wall. Ph.D. dissertation, Chinese Academy of Forestry, Beijing, China
Crow E, Murphy R (2000) Microfibril orientation in differentiating and maturing fibre and parenchyma cell walls in culms of bamboo (Phyllostachys viridi-glaucescens (Carr.) Riv. & Riv.). Bot J Linn Soc 134:339–359. doi:10.1111/j.1095-8339.2000.tb02357.x
Dixon PG, Gibson LJ (2014) The structure and mechanics of Moso bamboo material. J R Soc Interface 11:20140321. doi:10.1098/rsif.2014.0321
Dixon PG, Ahvenainen P, Aijazi AN, Chen SH, Lin S, Augusciak PK, Gibson LJ (2015) Comparison of the structure and flexural properties of Moso, Guadua and Tre Gai bamboo. Constr Build Mater 90:11–17. doi:10.1016/j.conbuildmat.2015.04.042
Donaldson L, Hague J, Snell R (2001) Lignin distribution in coppice poplar, linseed and wheat straw. Holzforschung 55:379–385. doi:10.1515/HF.2001.063
Eder M, Lütz-Meindl U, Weiss IM (2010) Non-invasive LC-PolScope imaging of biominerals and cell wall anisotropy changes. Protoplasma 246:49–64. doi:10.1007/s00709-010-0124-x
French AD (2014) Idealized powder diffraction patterns for cellulose polymorphs. Cellulose 21(2):885–896. doi:10.1007/s10570-013-0030-4
French AD, Santiago Cintrón M (2013) Cellulose polymorphy, crystallite size, and the Segal crystallinity index. Cellulose 20(1):583–588. doi:10.1007/s10570-012-9833-y
Gan X, Ding Y (2006) Investigation on the variation of fiber wall in Phyllostachys edulis culms. For Res 19:457
Gritsch CS, Murphy RJ (2005) Ultrastructure of fibre and parenchyma cell walls during early stages of culm development in Dendrocalamus asper. Ann Bot-Lond 95:619–629. doi:10.1093/aob/mci068
Gritsch CS, Kleist G, Murphy RJ (2004) Developmental changes in cell wall structure of phloem fibres of the bamboo Dendrocalamus asper. Ann Bot-Lond 94:497–505. doi:10.1093/aob/mch169
Gu Y, Kaplinsky N, Bringmann M, Cobb A, Carroll A, Sampathkumar A, Baskin TI, Persson S, Somerville CR (2010) Identification of a cellulose synthase-associated protein required for cellulose biosynthesis. Proc Natl Acad Sci USA 107(29):12866–12871. doi:10.1073/pnas.1007092107
He X-Q, Suzuki K, Kitamura S, Lin J-X, Cui K-M, Itoh T (2002) Toward understanding the different function of two types of parenchyma cells in bamboo culms. Plant Cell Physiol 43:186–195. doi:10.1093/pcp/pcf027
Huang Y, Fei B, Wei P, Zhao C (2016) Mechanical properties of bamboo fiber cell walls during the culm development by nanoindentation. Ind Crop Prod 92:102–108. doi:10.1016/j.indcrop.2016.07.037
Iyer K, Neelakantan P, Radhakrishnan T (1968) Birefringence of native cellulosic fibers. I. Untreated cotton and ramie. J Polym Sci Phys 6:1747–1758. doi:10.1002/pol.1968.160061005
Jiang Z (2007) Bamboo and Rattan in the World. China Forestry Publish House, Beijing
Jiang J, Yang Z, Zhu L, Shi L, Yan L (2008) Structure and property of bamboo fiber. J Beijing For Univ 30:128–132
Khalil HA, Bhat I, Jawaid M, Zaidon A, Hermawan D, Hadi Y (2012) Bamboo fibre reinforced biocomposites: a review. Mater Des 42:353–368. doi:10.1016/j.matdes.2012.06.015
Kim JS, Lee KH, Cho CH, Koch G, Kim YS (2008) Micromorphological characteristics and lignin distribution in bamboo (Phyllostachys pubescens) degraded by the white rot fungus Lentinus edodes. Holzforschung 62:481–487. doi:10.1515/HF.2008.080
Kinumoto T, Matsumura T, Yamaguchi K, Matsuoka M, Tsumura T, Toyoda M (2015) Material Processing of Bamboo for Use as a Gas Diffusion Layer in Proton Exchange Membrane Fuel Cells. ACS Sustain Chem Eng 3:1374–1380. doi:10.1021/acssuschemeng.5b00115
Kishi K, Harada H, Saiki H (1979) An electron microscopic study of the layered structure of the secondary wall in vessels. J Jap Wood Res Soc 25:521–527
Liese W (1998) The anatomy of bamboo culms, vol 18. Brill Academic Publishers, Leiden
Liese W (2005) Preservation of a bamboo culm in relation to its structure. World Bamboo Rattan 3:17–21
Liu B (2008) Formation of cell wall in development culms of Phyllostachys pubescens. Ph.D. dissertation, Chinese Academy of Forestry, Beijing, China
Liu D, Song J, Anderson DP, Chang PR, Hua Y (2012) Bamboo fiber and its reinforced composites: structure and properties. Cellulose 19:1449–1480. doi:10.1007/s10570-012-9741-1
Lybeer B (2006) Age-related anatomical aspects of some temperate and tropical bamboo culms (Poaceae: Bambusoideae). Ph.D. dissertation, Ghent University
Lybeer B, Koch G (2005) A topocuemical and semiquantitative study of the lignification during ageing of bamboo culms (Phyllostachys Viridiglaucescens). IAWA J 26:99–110. doi:10.1163/22941932-90001605
Lybeer B, Koch G, Van Acker J, Goetghebeur P (2006) Lignification and cell wall thickening in nodes of Phyllostachys viridiglaucescens and Phyllostachys nigra. Ann Bot-Lond 97:529–539. doi:10.1093/aob/mcl016
Ma L, Ma N (1997) Study on variation in bamboo wood properties of Phyllostachys heterocycla var. pubescens. Sci Silv Sin 33:356–364
Mannan S, Zaffar M, Pradhan A, Basu S (2016) Measurement of microfibril angles in bamboo using Mueller matrix imaging. Appl Opt 55:8971–8978. doi:10.1364/AO.55.008971
Murphy R, Alvin K (1992) Variation in fibre wall structure in bamboo. IAWA J 13:403–410. doi:10.1163/22941932-90001296
Murphy R, Sulaiman O, Alvin K (1997) Ultrastructural aspects of cell wall organization in bamboos. In: Chapman GP (ed) The Bamboos. Linnean Society symposium series. Academic Press Limited, London, pp 305–312
Mustafa MT, Wahab R, Sudin M, Sulaiman O, Kamal NAM, Khalid I (2011) Anatomical and microstructures features of tropical bamboo Gigantochloa brang, G. levis, G. scotechinii and G. wrayi. Int J For Soil Eros 1(1):25–35
Nishiyama Y, Langan P, Chanzy H (2002) Crystal structure and hydrogen-bonding system in cellulose Iβ from synchrotron X-ray and neutron fiber diffraction. J Am Chem Soc 124(31):9074–9082. doi:10.1021/ja0257319
Nogata F, Takahashi H (1995) Intelligent functionally graded material: bamboo. Compos Eng 5:743–751. doi:10.1016/0961-9526(95)00037-N
Palombini FL, Kindlein W Jr, de Oliveira BF, de Araujo Mariath JE (2016) Bionics and design: 3D microstructural characterization and numerical analysis of bamboo based on X-ray microtomography. Mater Charact 120:357–368. doi:10.1016/j.matchar.2016.09.022
Parameswaran N, Liese W (1976) On the fine structure of bamboo fibres. Wood Sci Technol 10:231–246. doi:10.1007/BF00350830
Preston JM (1933) Relations between the refractive indices and the behaviour of cellulose fibres. Trans Faraday Soc 29:65–71
Preston R, Singh K (1950) The fine structure of bamboo fibres I. Optical properties and X-ray data. J Exp Bot 1(2):214–226
Preston R, Singh K (1952) The Fine Structure of Bamboo Fibres II. Refractive indices and wall density. J Exp Bot 3(8):162–169
Preston R, Hermans P, Weidinger A (1950) The crystalline-non-crystalline ratio in celluloses of biological interest. J Exp Bot 1(3):344–352
Ren D, Wang H, Yu Z, Wang H, Yu Y (2015) Mechanical imaging of bamboo fiber cell walls and their composites by means of peakforce quantitative nanomechanics (PQNM) technique. Holzforschung 69:975–984. doi:10.1515/hf-2014-0237
Segal L, Creely JJ, Martin AE Jr, Conrad CM (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractomete. Text Res J 29(10):786–794
Sharma R, Varshney V, Chauhan GS, Naithani S, Soni P (2009) Hydroxypropylation of cellulose isolated from bamboo (Dendrocalamus strictus) with respect to hydroxypropoxyl content and rheological behavior of the hydroxypropyl cellulose. J Appl Polym Sci 113:2450–2455. doi:10.1002/app.30205
Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultra Mol Struct Res 26:31–43
Sun Z, Yang J, Yu C (2007) Bamboo fiber density. Prog Text Sci Technol 1:029
Sun M, He H, Zeng N, Du E, Guo Y, Liu S, Ma H (2014) Characterizing the microstructures of biological tissues using Mueller matrix and transformed polarization parameters. Biomed Opt Express 5(12):4223–4234. doi:10.1364/BOE.5.004223
Takagi H, Takura R, Ichihara Y, Ochi S, Misawa H, Niki R (2003) The mechanical properties of bamboo fibers prepared by steam-explosion method. J Soc Mater Sci Jpn 52:353–356
Tian G (2015) The main influence factors of bamboo fiber mechanical properties. Ph.D. dissertation, Chinese Academy of Forestry, Beijing, China
Toba K, Nakai T, Shirai T, Yamamoto H (2015) Changes in the cellulose crystallinity of moso bamboo cell walls during the growth process by X-ray diffraction techniques. J Wood Sci 61:517–524. doi:10.1007/s10086-015-1490-y
Tono T, Ono K (1962) The layered structure and its morphological transformation by acid treatment. J Japanese Wood Res Soc 8:245–249
Wai N, Nanko H, Murakami K (1985) A morphological study on the behavior of bamboo pulp fibers in the beating process. Wood Sci Technol 19:211–222. doi:10.1007/BF00392050
Wang XQ, Li XZ, Ren HQ (2010) Variation of microfibril angle and density in moso bamboo (Phyllostachys pubescens). J Trop For Sci 22(1):88–96
Wang X, Ren H, Zhang B, Fei B, Burgert I (2012a) Cell wall structure and formation of maturing fibres of moso bamboo (Phyllostachys pubescens) increase buckling resistance. J R Soc Interface 9:988–996. doi:10.1098/rsif.2011.0462
Wang Y, Leppänen K, Andersson S, Serimaa R, Ren H, Fei B (2012b) Studies on the nanostructure of the cell wall of bamboo using X-ray scattering. Wood Sci Technol 46(1–3):317–332. doi:10.1007/s00226-011-0405-3
Wang X, Keplinger T, Gierlinger N, Burgert I (2014) Plant material features responsible for bamboo’s excellent mechanical performance: a comparison of tensile properties of bamboo and spruce at the tissue, fibre and cell wall levels. Ann Bot-Lond 114:1627–1635. doi:10.1093/aob/mcu180
Wang H, Zhang X, Jiang Z, Li W, Yu Y (2015) A comparison study on the preparation of nanocellulose fibrils from fibers and parenchymal cells in bamboo (Phyllostachys pubescens). Ind Crop Prod 71:80–88. doi:10.1016/j.indcrop.2015.03.086
Yu Y, Tian G, Wang H, Fei B, Wang G (2011) Mechanical characterization of single bamboo fibers with nanoindentation and microtensile technique. Holzforschung 65:113. doi:10.1515/hf.2011.009
Yu Y, Wang H, Lu F, Tian G, Lin J (2014) Bamboo fibers for composite applications: a mechanical and morphological investigation. J Mater Sci 49:2559–2566. doi:10.1007/s10853-013-7951-z
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This work was funded by the National Natural Science Foundation of China (Nos. 31500472 and 31370563).
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Hu, K., Huang, Y., Fei, B. et al. Investigation of the multilayered structure and microfibril angle of different types of bamboo cell walls at the micro/nano level using a LC-PolScope imaging system. Cellulose 24, 4611–4625 (2017). https://doi.org/10.1007/s10570-017-1447-y
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DOI: https://doi.org/10.1007/s10570-017-1447-y