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Investigating adsorption of bovine serum albumin on cellulosic substrates using magnetic resonance imaging

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Abstract

Cellulosic biomass is recalcitrant to enzymatic hydrolysis which greatly reduces the efficiency of biofuels production. Specifically, the lignin component of biomass is thought to provide non-productive binding sites for glycosyl hydrolases, effectively disabling the enzymes from completing further digestion. A thorough understanding of the adsorption rates of protein molecules on celluloses—especially lignocelluloses—is crucial to improving the cyclic steps of adsorption, diffusion, and reaction. We use magnetic resonance imaging (MRI) to detect concentrations of bovine serum albumin (BSA) in equilibrium with various cellulose substrates, including delignified and acid-treated lignocellulosic substrates. BSA is believed to be an effective adsorption blocker during enzymatic hydrolysis of lignocellulosics, and has been correlated with an increase in reaction yield. We found BSA to have little adsorption onto the chosen cellulose substrates in the low concentration range studied. Ultraviolet (UV) absorption measurements of reaction supernatants at 280 nm were used to confirm the MRI results for each of the substrate types. The advantages of the MRI technique are compared with that of the traditional UV measurement.

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Acknowledgments

Fiber length measurements were provided by Tom Lindström from Innventia AB, Stockholm, Sweden. The pretreated corn stover, along with the pretreatment details, was provided by Clare Dibble at the National Renewable Energy Laboratory in Golden, Colorado. Compositional analyses were conducted by Nardrapee Karuna at UC Davis. Partial financial support provided by the Center for Process Analysis and Control, University of Washington, Seattle, Washington.

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Authors and Affiliations

  1. Department of Chemical and Materials Science Engineering, University of California, One Shields Ave., Davis, CA, 95616, USA

    David M. Lavenson, Emilio J. Tozzi & Robert L. Powell

  2. Department of Food Science and Technology, University of California, One Shields Ave., Davis, CA, 95616, USA

    Michael J. McCarthy

  3. Department of Biological and Agricultural Engineering, University of California, One Shields Ave., Davis, CA, 95616, USA

    Michael J. McCarthy & Tina Jeoh

Authors
  1. David M. Lavenson
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  2. Emilio J. Tozzi
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  3. Michael J. McCarthy
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  4. Robert L. Powell
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  5. Tina Jeoh
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Correspondence to Tina Jeoh.

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Lavenson, D.M., Tozzi, E.J., McCarthy, M.J. et al. Investigating adsorption of bovine serum albumin on cellulosic substrates using magnetic resonance imaging. Cellulose 18, 1543–1554 (2011). https://doi.org/10.1007/s10570-011-9588-x

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  • DOI: https://doi.org/10.1007/s10570-011-9588-x

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