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Decellularized kidney capsule as a three-dimensional scaffold for tissue regeneration

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Abstract

Tissue regeneration is thought to have considerable promise with the use of scaffolds designed for tissue engineering. Although polymer-based scaffolds for tissue engineering have been used extensively and developed quickly, their ability to mimic the in-vivo milieu, overcome immunogenicity, and have comparable mechanical or biochemical properties has limited their capability for repair. Fortunately, there is a compelling method to get around these challenges thanks to the development of extracellular matrix (ECM) scaffolds made from decellularized tissues. We used ECM decellularized sheep kidney capsule tissue in our research. Using detergents such as Triton-X100 and sodium dodecyl sulfate (SDS), these scaffolds were decellularized. DNA content, histology, mechanical properties analysis, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), biocompatibility, hemocompatibility and scanning electron microscope (SEM) imaging were measured. The results showed that the three-dimensional (3D) structure of the ECM remained largely intact. The scaffolds mentioned above had several hydrophilic properties. The best biocompatibility and blood compatibility properties were reported in the SDS method of 0.5%. The best decellularization scaffold was introduced with 0.5% SDS. Therefore, it can be proposed as a scaffold that has ECM like natural tissue, for tissue engineering applications.

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Acknowledgements

The results described in this paper were part of a student thesis. This paper originated from an MD dissertation (research code: 4020769 and 4020857) and with the ethics code IR.KUMS.AEC.1402.261 and IR.KUMS.AEC.1402.239.

Funding

This paper was funded research deputy of Kermanshah University of Medical Sciences, Kermanshah, Iran.

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Authors and Affiliations

  1. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mohammad Rasool Khazaei, Azam Bozorgi, Mozafar Khazaei & Leila Rezakhani

  2. Department of Tissue Engineering, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mohammad Rasool Khazaei, Azam Bozorgi, Mozafar Khazaei & Leila Rezakhani

  3. Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Rawa Ibrahim & Rayan Faris

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  1. Mohammad Rasool Khazaei
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  2. Rawa Ibrahim
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  4. Azam Bozorgi
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  5. Mozafar Khazaei
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  6. Leila Rezakhani
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Contributions

M.R.K. and R.I. and R.F. and A.B. performed the dissection experiments, data measurement and the statistical analysis, and wrote the first draft of the manuscript. M.K. and L.R. contributed to conception and design of the study. All authors contributed to manuscript revision, read, and approved the submitted version.

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Correspondence to Leila Rezakhani.

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Khazaei, M.R., Ibrahim, R., Faris, R. et al. Decellularized kidney capsule as a three-dimensional scaffold for tissue regeneration. Cell Tissue Bank (2024). https://doi.org/10.1007/s10561-024-10136-1

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