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Comparison of the effects of preservation methods on structural, biological, and mechanical properties of the human amniotic membrane for medical applications

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Abstract

Amniotic membrane (AM), the innermost layer of the placenta, is an exceptionally effective biomaterial with divers applications in clinical medicine. It possesses various biological functions, including scar reduction, anti-inflammatory properties, support for epithelialization, as well as anti-microbial, anti-fibrotic and angio-modulatory effects. Furthermore, its abundant availability, cost-effectiveness, and ethical acceptability make it a compelling biomaterial in the field of medicine. Given the potential unavailability of fresh tissue when needed, the preservation of AM is crucial to ensure a readily accessible and continuous supply for clinical use. However, preserving the properties of AM presents a significant challenge. Therefore, the establishment of standardized protocols for the collection and preservation of AM is vital to ensure optimal tissue quality and enhance patient safety. Various preservation methods, such as cryopreservation, lyophilization, and air-drying, have been employed over the years. However, identifying a preservation method that effectively safeguards AM properties remains an ongoing endeavor. This article aims to review and discuss different sterilization and preservation procedures for AM, as well as their impacts on its histological, physical, and biochemical characteristics.

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Fig. 1

(Modified from Nejad et al. 2021)

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Acknowledgements

This study is related to the project No 1399/61273 from Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran. We also appreciate the “Student Research Committee” and “Research & Technology Chancellor” in Shahid Beheshti University of Medical Sciences for their financial support of this study.

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Authors and Affiliations

  1. Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Ameneh Jafari

  2. Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Ameneh Jafari & Mostafa Rezaei-Tavirani

  3. Department of Medical Biochemical Analysis, Cihan University-Erbil, Erbil, Kurdistan Region, Iraq

    Yousef Mirzaei

  4. Department of Nusring, Mergasour Technical Institute, Erbil Polytechnic University, Erbil, Iraq

    Ali Hussein Mer

  5. 9th Dey Manzariye Hospital, Isfahan University of Medical Sciences, Isfahan, Iran

    Zahra Jafari

  6. Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Hassan Niknejad

Authors
  1. Ameneh Jafari
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  2. Yousef Mirzaei
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  3. Ali Hussein Mer
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  4. Mostafa Rezaei-Tavirani
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  5. Zahra Jafari
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  6. Hassan Niknejad
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Contributions

AJ contributed to the investigation, drew all figures, and wrote the original draft of the manuscript. MRT and HN contributed to the conceptualization and supervised the study. AJ, YM, AHM, and ZJ contributed for editing and revision of text. All authors read and approved the final manuscript.

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Correspondence to Mostafa Rezaei-Tavirani.

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Jafari, A., Mirzaei, Y., Mer, A.H. et al. Comparison of the effects of preservation methods on structural, biological, and mechanical properties of the human amniotic membrane for medical applications. Cell Tissue Bank 25, 305–323 (2024). https://doi.org/10.1007/s10561-023-10114-z

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  • DOI: https://doi.org/10.1007/s10561-023-10114-z

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