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Evaluation the ability of acellular ovine small intestine submucosa to load and release of mineral pitch and its anti-inflammatory effects

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Abstract

Injury from the severe burn is exacerbated by a persistent inflammatory response. This response is mediated by cytokines and chemokines, which are released from various immune cells, including mast cells. In this study, the ability of the acellular ovine small intestine submucosa (AOSIS) to load and release of Mineral Pitch (MP) was first investigated, and it was found that the preparation of the scaffold by a modified method enables it to load and release water-soluble drugs. Then, 32 male Wistar rats were divided into four groups, a third-degree burn was created, and except for the control group, the others were treated with: AOSIS, WJ-MSCs seeded AOSIS, or AOSIS loaded with WJ-MSCs and MP. Wound sampling on the 5th day after treatment showed that the number of intact and degranulated mast cells in the treatment groups was associated with a decrease compared to the control group. In the last group, this decrease was the largest (and statically significant (p < 0.05)). Also, by measuring the level of inflammatory factors in blood serum, it was found that in the treatment groups compared to the control group, IL-10 was associated with an increase, and TNF-α was associated with a decrease. The changes in inflammatory factors were more significant (p < 0.05) in the last group. So, our results indicate that AOSIS loaded with WJ-MSCs and MP could be used as an innovative tissue-engineered device to control inflammatory condition during burn wound healing.

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References

  • Acharya S, Frotan M, Goel R, Tripathi S, Das P (1988) Pharmacological actions of Shilajit. Indian J Exp Biol 26(10):775–777

    CAS  PubMed  Google Scholar 

  • Agarwal SP, Khanna R, Karmarkar R, Anwer MK, Khar RK (2007) Shilajit: a review. Phytother Res: Int J Devoted Pharmacol Toxicol Eval Nat Prod Derivatives 21(5):401–405

    Article  Google Scholar 

  • Agnes Mary S, Giri DV (2015) Electrospun herbal nanofibrous wound dressings for skin tissue engineering. J Textile Inst 106(8):886–895

    Article  CAS  Google Scholar 

  • Alves APNN, Júnior EML, Piccolo NS, de Miranda MJB, Verde MEQL, Júnior AECF et al (2018) Study of tensiometric properties, microbiological and collagen content in nile tilapia skin submitted to different sterilization methods. Cell Tissue Banking 19(3):373–382

    Article  CAS  PubMed  Google Scholar 

  • Andrade MG, Weissman R, Reis SR (2006) Tissue reaction and surface morphology of absorbable sutures after in vivo exposure. J Mater Sci: Mater Med 17(10):949–961

    CAS  Google Scholar 

  • Annan K, Houghton PJ (2008) Antibacterial, antioxidant and fibroblast growth stimulation of aqueous extracts of Ficus asperifolia Miq. and Gossypium arboreum L., wound-healing plants of Ghana. J Ethnopharmacol. 119(1):141–144

    Article  PubMed  Google Scholar 

  • Arima Y, Iwata H (2007) Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers. Biomaterials 28(20):3074–3082

    Article  CAS  PubMed  Google Scholar 

  • Ashley RA, Palmer BW, Schultz AD, Woodson BW, Roth CC, Routh JC et al (2009) Leukocyte inflammatory response in a rat urinary bladder regeneration model using porcine small intestinal submucosa scaffold. Tissue Eng Part A 15(11):3241–3246

    Article  CAS  PubMed  Google Scholar 

  • Beilin B, Shavit Y, Trabekin E, Mordashev B, Mayburd E, Zeidel A et al (2003) The effects of postoperative pain management on immune response to surgery. Anesthesia Analgesia 97(3):822–827

    Article  PubMed  Google Scholar 

  • Bhattacharya SK, Sen AP, Ghosal S (1995) Effects of Shilajit on biogenic free radicals. Phytother Res 9(1):56–59

    Article  Google Scholar 

  • Bundoc VG, Keane-Myers A (2007) IL-10 confers protection from mast cell degranulation in a mouse model of allergic conjunctivitis. Exp Eye Res 85(4):575–579

    Article  CAS  PubMed  Google Scholar 

  • Campillo-Fernández AJ, Unger RE, Peters K, Halstenberg S, Santos M, Sánchez MS et al (2009) Analysis of the biological response of endothelial and fibroblast cells cultured on synthetic scaffolds with various hydrophilic/hydrophobic ratios: influence of fibronectin adsorption and conformation. Tissue Eng Part A 15(6):1331–1341

    Article  PubMed  Google Scholar 

  • Chang K-C, Ma H, Liao W-C, Lee C-K, Lin C-Y, Chen C-C (2010) The optimal time for early burn wound excision to reduce pro-inflammatory cytokine production in a murine burn injury model. Burns 36(7):1059–1066

    Article  PubMed  Google Scholar 

  • Chelombitko M, Fedorov A, Ilyinskaya O, Zinovkin R, Chernyak B (2016) Role of reactive oxygen species in mast cell degranulation. Biochem Mosc 81(12):1564–1577

    Article  CAS  Google Scholar 

  • Church D, Elsayed S, Reid O, Winston B, Lindsay R (2006) Burn wound infections. Clin Microbiol Rev 19(2):403–434

    Article  PubMed  PubMed Central  Google Scholar 

  • Cruz FF, Rocco PR (2017) Stem-cell extracellular vesicles and lung repair. Stem Cell Invest 4:78

    Article  CAS  Google Scholar 

  • da Silva ML, Fontes AM, Covas DT, Caplan AI (2009) Mechanisms involved in the therapeutic properties of mesenchymal stem cells. Cytokine Growth Factor Rev 20(5–6):419–427

    Google Scholar 

  • Dahlin RL, Kasper FK, Mikos AG (2011) Polymeric nanofibers in tissue engineering. Tissue Eng Part B: Rev 17(5):349–364

    Article  CAS  Google Scholar 

  • De La Puente P, Ludeña D, Fernández A, Aranda JL, Varela G, Iglesias J (2011) Autologous fibrin scaffolds cultured dermal fibroblasts and enriched with encapsulated bFGF for tissue engineering. J Biomed Mater Res Part A 99(4):648–654

    Article  CAS  Google Scholar 

  • Elcin AE, Elcin YM (2006) Localized angiogenesis induced by human vascular endothelial growth factor-activated PLGA sponge. Tissue Eng 12(4):959–968

    Article  CAS  PubMed  Google Scholar 

  • Evers LH, Bhavsar D, Mailänder P (2010) The biology of burn injury. Exp Dermatol 19(9):777–783

    Article  PubMed  Google Scholar 

  • FerdowsiKhosroshahi A, Soleimani Rad J, Kheirjou R, Roshangar B, Rashtbar M, Salehi R et al (2020) Adipose tissue-derived stem cells upon decellularized ovine small intestine submucosa for tissue regeneration: an optimization and comparison method. J Cell Physiol 235(2):1556–1567

    Article  CAS  Google Scholar 

  • Finnerty CC, Przkora R, Herndon DN, Jeschke MG (2009) Cytokine expression profile over time in burned mice. Cytokine 45(1):20–25

    Article  CAS  PubMed  Google Scholar 

  • Frangogiannis NG, Perrard JL, Mendoza LH, Burns AR, Lindsey ML, Ballantyne CM et al (1998) Stem cell factor induction is associated with mast cell accumulation after canine myocardial ischemia and reperfusion. Circulation 98(7):687–698

    Article  CAS  PubMed  Google Scholar 

  • Gauglitz GG, Song J, Herndon DN, Finnerty CC, Boehning D, Barral JM et al (2008) Characterization of the inflammatory response during acute and post-acute phases after severe burn. Shock 30(5):503–507

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gholipour-Kanani A, Bahrami S, Samadi-Kochaksaraie A, Ahmadi-Tafti H, Rabbani S, Kororian A et al (2012) Effect of tissue-engineered chitosan-poly (vinyl alcohol) nanofibrous scaffolds on healing of burn wounds of rat skin. IET Nanobiotechnol 6(4):129–135

    Article  CAS  PubMed  Google Scholar 

  • Ghosal S (1990) Chemistry of shilajit, an immunomodulatory ayurvedic rasayan. Pure Appl Chem 62(7):1285–1288

    Article  CAS  Google Scholar 

  • Ghosal S, Lal J, Singh SK, Dasgupta G, Bhaduri J, Mukhopadhyay M et al (1989) Mast cell protecting effects of shilajit and its constituents. Phytother Res 3(6):249–252

    Article  CAS  Google Scholar 

  • Ghosal S, Lata S, Kumar Y, Gaur B, Misra N, Soumya L et al (1995) Interaction of Shilajit with biogenic free radicals. Indian J Chem Sect B-Organic Chem Include Med Chem 34:596–602

    Google Scholar 

  • Goel R, Banerjee R, Acharya S (1990) Antiulcerogenic and antiinflammatory studies with shilajit. J Ethnopharmacol 29(1):95–103

    Article  CAS  PubMed  Google Scholar 

  • Houghton P, Hylands P, Mensah A, Hensel A, Deters A (2005) In vitro tests and ethnopharmacological investigations: wound healing as an example. J Ethnopharmacol 100(1–2):100–107

    Article  CAS  PubMed  Google Scholar 

  • Hristova M, Bekyarova G, Atanasova M, Tzaneva M (2018) Melatonin attenuates oxidative stress and modulates inflammatory response after experimental burn trauma. J Mind Med Sci 5(1):93–100

    Article  Google Scholar 

  • John ALS, Abraham SN (2013) Innate immunity and its regulation by mast cells. J Immunol 190(9):4458–4463

    Article  CAS  Google Scholar 

  • Kang SK, Shin IS, Ko MS, Jo JY, Ra JC (2012) Journey of mesenchymal stem cells for homing: strategies to enhance efficacy and safety of stem cell therapy. Stem Cells Int. https://doi.org/10.1155/2012/342968

    Article  PubMed  PubMed Central  Google Scholar 

  • Karahuseyinoglu S, Cinar O, Kilic E, Kara F, Akay GG, Demiralp DÖ et al (2007) Biology of stem cells in human umbilical cord stroma: in situ and in vitro surveys. Stem Cells 25(2):319–331

    Article  CAS  PubMed  Google Scholar 

  • Karuppuswamy P, Venugopal JR, Navaneethan B, Laiva AL, Sridhar S, Ramakrishna S (2014) Functionalized hybrid nanofibers to mimic native ECM for tissue engineering applications. Appl Surface Sci 322:162–168

    Article  CAS  Google Scholar 

  • Katz S, Izhar M, Mirelman D (1981) Bacterial adherence to surgical sutures. A possible factor in suture induced infection. Ann Surg 194(1):35

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kheirjou R, Rad JS, Khosroshahi AF, Roshangar L (2020) The useful agent to have an ideal biological scaffold. Cell Tissue Bank. https://doi.org/10.1007/s10561-020-09881-w

    Article  PubMed  Google Scholar 

  • Khorramirouz R, Go JL, Noble C, Jana S, Maxson E, Lerman A et al (2018) A novel surgical technique for a rat subcutaneous implantation of a tissue engineered scaffold. Acta Histochem 120(3):282–291

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kim TG, Do Ki K, Lee M-K, So J-W, Chung SK, Kang J (2019) Comparison of cytokine expression and ultrastructural alterations in fresh-frozen and dried electron beam-irradiated human amniotic membrane and chorion. Cell Tissue Bank 20(2):163–172

    Article  PubMed  Google Scholar 

  • Kubo H, Hayashi T, Ago K, Ago M, Kanekura T, Ogata M (2014) Temporal expression of wound healing-related genes in skin burn injury. Legal Med 16(1):8–13

    Article  CAS  PubMed  Google Scholar 

  • Kulac M, Aktas C, Tulubas F, Uygur R, Kanter M, Erboga M et al (2013) The effects of topical treatment with curcumin on burn wound healing in rats. J Mol Histol 44(1):83–90

    Article  CAS  PubMed  Google Scholar 

  • Leclerc T, Thepenier C, Jault P, Bey E, Peltzer J, Trouillas M et al (2011) Cell therapy of burns. Cell Prolif 44:48–54

    Article  PubMed  PubMed Central  Google Scholar 

  • Li X, Liu L, Yang J, Yu Y, Chai J, Wang L et al (2016) Exosome derived from human umbilical cord mesenchymal stem cell mediates MiR-181c attenuating burn-induced excessive inflammation. EBioMedicine 8:72–82

    Article  PubMed  PubMed Central  Google Scholar 

  • Liang M, Chen Z, Wang F, Liu L, Wei R, Zhang M (2019) Preparation of self-regulating/anti-adhesive hydrogels and their ability to promote healing in burn wounds. J Biomed Mater Res Part B: Appl Biomater 107(5):1471–1482

    Article  CAS  Google Scholar 

  • Linenberger M, Jacobson F, Bennett L, Broudy V, Martin F, Abkowitz J (1995) Stem cell factor production by human marrow stromal fibroblasts. Exp Hematol 23(10):1104

    CAS  PubMed  Google Scholar 

  • Liu M-L, Mars WM, Zarnegar R, Michalopoulos GK (1994) Uptake and distribution of hepatocyte growth factor in normal and regenerating adult rat liver. Am J Pathol 144(1):129

    CAS  PubMed  PubMed Central  Google Scholar 

  • Liu P, Deng Z, Han S, Liu T, Wen N, Lu W et al (2008) Tissue-engineered skin containing mesenchymal stem cells improves burn wounds. Artif Organs 32(12):925–931

    Article  PubMed  Google Scholar 

  • Liu L, Yu Y, Hou Y, Chai J, Duan H, Chu W et al (2014) Human umbilical cord mesenchymal stem cells transplantation promotes cutaneous wound healing of severe burned rats. PLoS ONE 9(2):e88348

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Liu L, Song H, Duan H, Chai J, Yang J, Li X et al (2016) TSG-6 secreted by human umbilical cord-MSCs attenuates severe burn-induced excessive inflammation via inhibiting activations of P38 and JNK signaling. Sci Rep 6:30121

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mantle D, Gok MA, Lennard T (2001) Adverse and beneficial effects of plant extracts on skin and skin disorders. Adverse Drug React Toxicol Rev 20(2):89–103

    CAS  PubMed  Google Scholar 

  • Marshall JS (2004) Mast-cell responses to pathogens. Nat Rev Immunol 4(10):787–799

    Article  CAS  PubMed  Google Scholar 

  • Marshall JD, Aste-Amézaga M, Chehimi SS, Murphy M, Olsen H, Trinchieri G (1999) Regulation of human IL-18 mRNA expression. Clin Immunol 90(1):15–21

    Article  CAS  PubMed  Google Scholar 

  • Moon TC, Befus AD, Kulka M (2014) Mast cell mediators: their differential release and the secretory pathways involved. Front Immunol 5:569

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Moreno E, Meneu J, Calvo J, Pérez B, Sesma A, Manrique A et al (2005) Modulation of hepatocyte growth factor plasma levels in relation to the dose of exogenous heparin administered: an experimental study in rats. In: Transplantation proceedings. Elsevier, Amsterdam, pp 3943–3947.

  • Norton SK, Barnstein B, Brenzovich J, Bailey DP, Kashyap M, Speiran K et al (2008) IL-10 suppresses mast cell IgE receptor expression and signaling in vitro and in vivo. J Immunol 180(5):2848–2854

    Article  Google Scholar 

  • Ohno T, Hirano S, Kanemaru S-I, Yamashita M, Umeda H, Suehiro A et al (2007) Drug delivery system of hepatocyte growth factor for the treatment of vocal fold scarring in a canine model. Ann Otol Rhinol Laryngol 116(10):762–769

    Article  PubMed  Google Scholar 

  • Ono I, Gunji H, Zhang J, Maruyama K, Kaneko F (1995) A study of cytokines in burn blister fluid related to wound healing. Burns 21(5):352–355

    Article  CAS  PubMed  Google Scholar 

  • Orman MA, Nguyen TT, Ierapetritou MG, Berthiaume F, Androulakis IP (2011) Comparison of the cytokine and chemokine dynamics of the early inflammatory response in models of burn injury and infection. Cytokine 55(3):362–371

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Park J-S, Kim G-Y, Han K (2006) The spermatogenic and ovogenic effects of chronically administered Shilajit to rats. J Ethnopharmacol 107(3):349–353

    Article  PubMed  Google Scholar 

  • Peck MD, Heimbach DM (1989) Does early excision of burn wounds change the pattern of mortality? J Burn Care Rehab 10(1):7–10

    Article  CAS  Google Scholar 

  • Prasanna SJ, Gopalakrishnan D, Shankar SR, Vasandan AB (2010) Pro-inflammatory cytokines, IFNγ and TNFα, influence immune properties of human bone marrow and Wharton jelly mesenchymal stem cells differentially. PLoS ONE 5(2):e9016

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Rachana S (2009) Antifungal activity screening and HPLC analysis of crude extract from Tectona grandis, Shilajit, Valeriana wallachi. Electron J Environ Agric Food Chem 8(4):218–229

    Google Scholar 

  • Rashtbar M, Hadjati J, Ai J, Shirian S, Jahanzad I, Azami M et al (2018) Critical-sized full-thickness skin defect regeneration using ovine small intestinal submucosa with or without mesenchymal stem cells in rat model. J Biomed Mater Res Part B: Appl Biomater 106(6):2177–2190

    Article  CAS  Google Scholar 

  • Rehou S, Shahrokhi S, Natanson R, Stanojcic M, Jeschke MG (2017) Antioxidant and trace element supplementation reduce the inflammatory response in critically ill burn patients. J Burn Care Res 39(1):1–9

    Article  PubMed Central  Google Scholar 

  • Ribeiro NF, Heath CH, Kierath J, Rea S, Duncan-Smith M, Wood FM (2010) Burn wounds infected by contaminated water: case reports, review of the literature and recommendations for treatment. Burns 36(1):9–22

    Article  PubMed  Google Scholar 

  • Sabetkam S, Soleimani Rad J, Hassan Pour Khodaie S, Roshangar L (2018) Impact of mummy substance on proliferation and migration of human wharton’s jelly-derived stem cells and fibroblasts in vitro culture system. Crescent J Med Biol Sci. 5(3):233–240

    CAS  Google Scholar 

  • Schottelius AJ, Mayo MW, Sartor RB, Baldwin AS (1999) Interleukin-10 signaling blocks inhibitor of κB kinase activity and nuclear factor κB DNA binding. J Biol Chem 274(45):31868–31874

    Article  CAS  PubMed  Google Scholar 

  • Silva EA, Kim E-S, Kong HJ, Mooney DJ (2008) Material-based deployment enhances efficacy of endothelial progenitor cells. Proc Natl Acad Sci 105(38):14347–14352

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Standiford TJ, Strieter RM, Lukacs NW, Kunkel SL (1995) Neutralization of IL-10 increases lethality in endotoxemia. Cooperative effects of macrophage inflammatory protein-2 and tumor necrosis factor. J Immunol. 155(4):2222–2229

    Article  CAS  PubMed  Google Scholar 

  • Summer GJ, Romero-Sandoval EA, Bogen O, Dina OA, Khasar SG, Levine JD (2008) Proinflammatory cytokines mediating burn-injury pain. PAIN® 135(1–2):98–107

    Article  CAS  Google Scholar 

  • Sun G, Zhang X, Shen Y-I, Sebastian R, Dickinson LE, Fox-Talbot K et al (2011) Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing. Proc Natl Acad Sci 108(52):20976–20981

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tompkins RG, Burke JF, Schoenfeld DA, Bondoc CC, Quinby WC Jr, Behringer GC et al (1986) Prompt eschar excision: a treatment system contributing to reduced burn mortality. A statistical evaluation of burn care at the Massachusetts General Hospital (1974–1984). Ann Surg 204(3):272

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Turner NA, Mughal RS, Warburton P, O’Regan DJ, Ball SG, Porter KE (2007) Mechanism of TNFα-induced IL-1α, IL-1β and IL-6 expression in human cardiac fibroblasts: effects of statins and thiazolidinediones. Cardiovasc Res 76(1):81–90

    Article  CAS  PubMed  Google Scholar 

  • Weil BR, Markel TA, Herrmann JL, Abarbanell AM, Kelly ML, Meldrum DR (2009) Stem cells in sepsis. Ann Surg 250(1):19–27

    Article  PubMed  Google Scholar 

  • Wernersson S, Pejler G (2014) Mast cell secretory granules: armed for battle. Nat Rev Immunol 14(7):478–494

    Article  CAS  PubMed  Google Scholar 

  • Zhou C, Yang B, Tian Y, Jiao H, Zheng W, Wang J et al (2011) Immunomodulatory effect of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells on lymphocytes. Cell Immunol 272(1):33–38

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

We thank the staff of Stem Cell Research Center and Tissue Engineering, Lab of Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Funding

Faculty of Medicine and Stem Cell Research Center, Tabriz University of Medical Sciences (Grant No. 1395.1339).

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Authors and Affiliations

  1. Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Raziyeh Kheirjou & Ahad Ferdowsi Khosroshahi

  2. Stem Cell Research Center, Tabriz University of Medical Sciences, Golgasht Street, 51376563833, Tabriz, Iran

    Jafar Soleimani Rad & Leila Roshangar

  3. Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

    Soodabeh Davaran

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  1. Raziyeh Kheirjou
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  2. Jafar Soleimani Rad
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  5. Leila Roshangar
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Contributions

RKH: participated in executing the pilot; LR: contributed to the design and executive process; RKH and LR: involved in writing and editing the manuscript and preparation figures; JSR: participated in the design and conception; AFKH: participated in executing the pilot; SD: conducted in load and release technique. All authors read and approved the final manuscript.

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Correspondence to Leila Roshangar.

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This study protocol was reviewed and approved by [Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran], approval number [1395.1339].

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Kheirjou, R., Rad, J.S., Khosroshahi, A.F. et al. Evaluation the ability of acellular ovine small intestine submucosa to load and release of mineral pitch and its anti-inflammatory effects. Cell Tissue Bank 23, 541–555 (2022). https://doi.org/10.1007/s10561-021-09985-x

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