Alginate and chitosan particles are drug delivery system for cell therapy

The publisher regrets that the following figures published in Biomedical Microdevices 10:2, pp. 131–140 by Gianni Ciofani, Vittoria Raffa, Arianna Menciassi and Paolo Dario should have printed in color. Please see below for color figures.

Fig. 1

(a) FIB imaging of alginate particles; (b) FIB imaging of chitosan particles; (c) size distribution of alginate particles (mean radius $\overline r = 650\,{\text{nm}}$, standard deviation σ = 230 nm); (d) size distribution of chitosan particles (mean radius $\overline r = 650\,{\text{nm}}$, standard deviation σ = 270 nm)

Fig. 2

HN9.10e cells up-take of alginate (a, b, c) and chitosan (d, e, f) particles: cells after 4 h of incubation with particles (a, d); cells after 8 h (b, e) and after 20 h (c, f) of incubation with particle-free growth medium. Magnification ×40. Circles underline particles within the cells

Fig. 3

CrFK cells up-take of alginate (a, b, c) and chitosan (d, e, f) particles: cells after 4 h of incubation with particles (a, d); cells after 8 h (b, e) and after 20 h (c, f) of incubation with particle-free growth medium. Magnification ×40. Circles underline particles within the cells

Fig. 5

Fluorescein loaded particles up-taken by HN9.10e cells. Fluorescent and phase-contrast images (×20) respectively of alginate particles (a, b) and chitosan particles (c, d). Fluorescent and phase-contrast images (×60) of alginate particles inside a HN9.10e cell (e, f)

Fig. 6

Fluorescein loaded particles up-taken by CrFK cells. Fluorescent and phase-contrast images (×20) respectively of alginate particles (a, b) and chitosan particles (c, d). Fluorescent and phase-contrast images (×60) of alginate particles inside a CrFK cell (e, f)

Fig. 7

Fluorescent images of lysosomal compartments of HN9.10e cells incubated with alginate particles (a), chitosan particles (c) and without particles (e). (b), (d) and (f) are the respective phase-contrast images. Magnification ×20