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Regulation of NAD+-dependent isocitrate dehydrogenase in the citrate producing yeast yarrowia lipolytica

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Abstract

The mechanism of the increased accumulation (overproduction) of citric acids in the yeast Yarrowia lipolytica while growing in the presence of glucose under nitrogen deficiency was investigated. The limitation of the yeast growth by the source of nitrogen decreases the total content of nucleotides and increases the ratios of ATP/AMP and NADH/NAD+. NAD+-Dependent isocitrate dehydrogenase, an enzyme of the tricarboxylic acid cycle playing a key role in the regulation of biosynthesis of citric and isocitric acids, was isolated from Y. lipolytica. The molecular weights of the native enzyme and its subunits were found to be 412 and 52 kD, respectively. It is concluded that the enzyme is a homooligomer consisting of eight subunits. Investigation of the effect of some intermediates of the tricarboxylic acid cycle on the activity of this enzyme suggests that the enhanced excretion of citric acids can be caused by the inhibition of NAD+-dependent isocitrate dehydrogenase due to the decrease in the content of AMP and increase in the NADH/NAD+ ratio in the cells of Y. lipolytica under depletion of nitrogen.

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REFERENCES

  1. C. Ratledge (1978) NoChapterTitle A. H. Rose (Eds) Economic Microbiology Academic Press N. Y. London 263–301

    Google Scholar 

  2. T. V. Finogenova A. V. Grinchak V. I. Illarionova N. V. Shishkanova (1979) Prikl. Biokhim. Mikrobiol. 15 811–816

    Google Scholar 

  3. T. V. Finogenova N. V. Shishkanova I. A. Kataeva (1989) Mikrobiologiya 58 387–392

    Google Scholar 

  4. T. V. Finogenova (1982) Doctoral dissertation IBFM Pushchino

    Google Scholar 

  5. Finogenova, T. V. (1991) in Metabolism of Alkanes and Oversynthesis of Products by Microorganisms [in Russian], Pushchino, pp. 96–114.

  6. P. R. Burkholder (1943) Amer. J. Botany 30 206

    Google Scholar 

  7. J. W. T. Wimpenny A. J. Firth (1972) J. Bacteriol. 111 24–32

    Google Scholar 

  8. G. A. Kimmich J. Randless J. S. Brand (1975) Analyt. Biochem. 69 187–206

    Google Scholar 

  9. C. Bernofsky M. Swan (1973) Analyt. Biochem. 53 452–458

    Google Scholar 

  10. P. A. Srere (1969) Meth. Enzymol. 13 3–11

    Google Scholar 

  11. C. B. Anfinsen (1955) Meth. Enzymol. 1 695–698

    Google Scholar 

  12. A. Kornberg W. E. J. Pricer (1951) J. Biol. Chem. 189 123–126

    Google Scholar 

  13. G. H. Dixon H. L. Kornberg (1959) Biochem. J. 72 3

    Google Scholar 

  14. A. K. Romanova (1980) Biochemical Methods of Study of Autotrophism in Microorganisms Nauka Moscow

    Google Scholar 

  15. M. M. Bradford (1976) Analyt. Biochem. 72 248–254

    Google Scholar 

  16. P. A. Botham C. J. Ratledge (1979) Gen. Microbiol. 114 361–375

    Google Scholar 

  17. L. Webb (1966) Inhibitors of Enzymes and Metabolism. General Principles of Inhibition Mir Moscow

    Google Scholar 

  18. C. L. Slayman (1973) J. Bacteriol. 114 752–766

    Google Scholar 

  19. D. E. Atkinson (1977) Cellular Energy Metabolism and Its Regulation Academic Press N. Y. San Francisco-London

    Google Scholar 

  20. C. J. Knowles (1977) Symp. Soc. Gen. Microbiol. 27 241–283

    Google Scholar 

  21. L. D. Barnes J. J. McGuire D. E. Atkinson (1972) Biochemistry 11 4322–4329

    Google Scholar 

  22. C. T. Evans C. Ratledge (1985) Can. J. Microbiol. 31 845–850

    Google Scholar 

  23. D. M. Sokolov N. Y. Solodovnikova A. A. Sharyshev T. B. Finogenova (1995) Prikl. Biokhim. Mikrobiol. 31 528–533

    Google Scholar 

  24. J. A. Hathaway D. E. Atkinson (1963) J. Biol. Chem. 238 2875–2881

    Google Scholar 

  25. D. E. Atkinson J. A. Hathaway (1965) J. Biol. Chem. 240 2682–2690

    Google Scholar 

  26. J. L. Gabriel C. W. E. Plaut (1991) Biochemistry 30 2594–2599

    Google Scholar 

  27. J. L. Gabriel C. W. E. Plaut (1990) Biochemistry 29 3528–3533

    Google Scholar 

  28. A. P. Lin M. T. McCammon L. McAlister-Henn (2001) Biochemistry 40 14291–14301

    Google Scholar 

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Correspondence to I. G. Morgunov.

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Translated from Biokhimiya, Vol. 69, No. 12, 2004, pp. 1706–1714.

Original Russian Text Copyright © 2004 by Morgunov, Solodovnikova, Sharyshev, Kamzolova, Finogenova.

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Morgunov, I.G., Solodovnikova, N.Y., Sharyshev, A.A. et al. Regulation of NAD+-dependent isocitrate dehydrogenase in the citrate producing yeast yarrowia lipolytica . Biochemistry (Moscow) 69, 1391–1398 (2004). https://doi.org/10.1007/s10541-005-0011-9

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  • DOI: https://doi.org/10.1007/s10541-005-0011-9

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