biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 60:269-278, 2016 | DOI: 10.1007/s10535-016-0586-6

Characterization of S-adenosylmethionine synthetases in soybean under flooding and drought stresses

X. Wang1,2, M. W. Oh1,2, S. Komatsu1,2,*
1 Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
2 National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, Japan

Soybean is stress-sensitive crop that exhibits markedly reduced growth under flooding and drought conditions. Three S-adenosylmethionine synthetases (SAMs) proteins were identified as flooding and drought responsive proteins in soybean using a proteomic technique. To better understand the role of these SAMs proteins in soybean under flooding and drought stresses, temporal, organ, and stress specificities were examined at mRNA and enzyme activity levels. The activity of SAMs decreased in response to the flooding, however, it was not significantly changed by NaCl, cold, gibberellic acid, and calcium in soybean roots. The activity of SAMs was induced in roots and hypocotyls under drought. The mRNA expression of the S-adenosylmethionine synthetase (SAMs) family was down-regulated in root tips and roots under the flooding and the drought, and SAMs 1 and SAMs 2 were down-regulated in roots under both stresses. A gene 1-aminocyclopropane-1-carboxylate synthase was up-regulated in root tips, roots, and hypocotyls under drought, however, it was not changed in root tips and roots under the flooding. In addition, 1-aminocyclopropane-1-carboxylate oxidase was induced in root tips under flooding and drought. These results suggest that SAMs was involved in the response to the flooding and drought and it might affect ethylene biosynthesis in soybean.

Keywords: 1-aminocyclopropane-1-carboxylate synthase; 1-aminocyclopropane-1-carboxylate oxidase
Subjects: S-adenosylmethionine synthetase; flooding; drought; amino acid sequence; phylogenetic analysis; soybean

Received: March 26, 2015; Revised: September 29, 2015; Accepted: October 5, 2015; Published: June 1, 2016  Show citation

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Wang, X., Oh, M.W., & Komatsu, S. (2016). Characterization of S-adenosylmethionine synthetases in soybean under flooding and drought stresses. Biologia plantarum, 60(2), 269-278. doi: 10.1007/s10535-016-0586-6
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