biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:792-797, 2014 | DOI: 10.1007/s10535-014-0449-y

Marker assisted gene pyramiding for enhanced Tomato leaf curl virus disease resistance in tomato cultivars

A. Kumar1,4,*, K. L. Tiwari2, D. Datta3,4, M. Singh4
1 Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
2 Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, Bilaspur, C.G., India
3 Division of Crop Improvement, Indian Institute of Pulses Research, Kalyanpur, Kanpur, U.P., India
4 Division of Crop Improvement, Indian Institute of Vegetable Research, Varanasi, U.P., India

The present research is aimed towards molecular marker assisted pyramiding Tomato leaf curl virus (ToLCV) disease resistance genes into two ToLCV susceptible tomato (Solanum lycopersicum L.) cvs. Pbc and H-86 (resistance genes recipient parents). Resistance gene donors were EC-538408 (Solanum chilense) and EC-520061 (S. peruvianum) in the case of cv. Pbc, and EC-520061 (S. peruvianum) and H-24 (S. lycopersicum) in the case of cv. H-86. A ToLCV resistance gene associated co-dominant simple sequence repeat (SSR) marker SSR-218 was used to discriminate between homozygotes and heterozygotes at the seedling stage prior to pollination, which enabled the rejection of nontarget back crosses and pyramiding progenies of the crosses PbcxEC-520061 and H-86xEC-520061, whereas SSR-306 was used for the cross PbcxEC-538408. Ty-2 gene cleaved amplified polymorphic sequences (CAPS) marker was used for the cross H-86xH-24. Out of 279 pyramiding progenies of the cross PbcxEC-538408/PbcxEC-520061, total 91 plants showed the presence of both resistance allele 1 and 2 along with both susceptibility alleles, and in 243 pyramiding progenies of the cross H-86xEC-520061/H-86xH-24, total 82 plants showed the presence of both resistance allele 1 and Ty-2 along with both susceptible alleles. The pyramiding lines that carried both pyramided resistance genes were resistant to tomato leaf curl disease throughout its life cycle.

Keywords: CAPS; molecular markers; Solanum lycopersicum; SSR
Subjects: gene pyramiding; disease resistance; molecular markers; simple sequence repeat; tomato

Received: July 27, 2013; Revised: June 8, 2014; Accepted: June 17, 2014; Published: December 1, 2014  Show citation

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Kumar, A., Tiwari, K.L., Datta, D., & Singh, M. (2014). Marker assisted gene pyramiding for enhanced Tomato leaf curl virus disease resistance in tomato cultivars. Biologia plantarum, 58(4), 792-797. doi: 10.1007/s10535-014-0449-y
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