biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:56-62, 2013 | DOI: 10.1007/s10535-012-0236-6

Molecular cloning and expression analysis of a new stress-related AREB gene from Arachis hypogaea

L. Hong1,2, B. Hu1, X. Liu1, C. Y. He1, Y. Yao1, X. L. Li1, L. Li1,*
1 Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou, P.R. China
2 College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, P.R. China

An AREB gene, designated as AhAREB1, was cloned from peanut (Arachis hypogaea L.). The gene contains a 1 338-bp open reading frame that encodes a putative protein of 445 amino acids. The corresponding genomic DNA containing four exons and three introns was isolated and analyzed. An upstream 1 060-bp DNA promoter fragment of the AhAREB1 gene was also amplified from peanut genomic DNA. Multiple sequence alignment of the deduced amino acids of AREB showed that the AhAREB1 protein shares high sequence homology with GmAREB1, S1AREB and ABF2. Quantitative real-time PCR analysis showed that AhAREB1 was induced by polyethylene glycol, NaCl, gibberellic acid, abscisic acid and salicylic acid. The cloning and characterization of the AhAREB1 gene will be useful for further studies establishing the biological role of AhAREB1 in plants.

Keywords: ABA; AhAREB1; GA3; peanut; PEG; SA; water stress
Subjects: gene expression; molecular cloning; stress-related genes; abscisic acid; abscisic acid response element; gibberellin; peanut; polyethylene glycol; salicylic acid; water stress

Received: May 25, 2011; Accepted: March 5, 2012; Published: March 1, 2013  Show citation

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Hong, L., Hu, B., Liu, X., He, C.Y., Yao, Y., Li, X.L., & Li, L. (2013). Molecular cloning and expression analysis of a new stress-related AREB gene from Arachis hypogaea. Biologia plantarum, 57(1), 56-62. doi: 10.1007/s10535-012-0236-6
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