biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:559-562, 2011 | DOI: 10.1007/s10535-011-0125-4

Improved growth and acclimatization of somatic embryo-derived Oplopanax elatus plantlets by ventilated photoautotrophic culture

S. Y. Park1,*, H. K. Moon1, H. N. Murthy2, Y. W. Kim1
1 Division of Forest Biotechnology, Korea Forest Research Institute, Suwon, Republic of Korea
2 Department of Botany, Karnatak University, Karnatak, India

To improve large-scale in vitro production of Oplopanax elatus Nakai, we cultured somatic embryo-derived plantlets under a heterotrophic condition (semi-solid culture with sucrose), photoautotrophic condition (semi-solid culture without sucrose), or modified photoautotrophic condition (liquid culture with forced ventilation). The plantlets grown under the modified photoautotrophic condition had more leaves as well as higher chlorophyll content, and higher net photosynthetic rate than those grown under the conventional conditions. Further, the photoautotrophically grown plantlets acclimatized better and sooner upon ex vitro transplantation than did the conventionally cultured plantlets. Consequently, a photoautotrophic culture method with forced ventilation is effective for enhancing the growth and acclimatization of O. elatus.

Keywords: ex vitro transfer; medicinal plant; large scale culture

Received: December 21, 2009; Accepted: April 9, 2010; Published: September 1, 2011  Show citation

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Park, S.Y., Moon, H.K., Murthy, H.N., & Kim, Y.W. (2011). Improved growth and acclimatization of somatic embryo-derived Oplopanax elatus plantlets by ventilated photoautotrophic culture. Biologia plantarum, 55(3), 559-562. doi: 10.1007/s10535-011-0125-4
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