biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:131-134, 2010 | DOI: 10.1007/s10535-010-0020-4

Efficient regeneration of Eucalyptus urophylla from seedling-derived hypocotyls

Z. -C. Huang1,2, F. -H. Zeng1,*, X. -Y. Lu2
1 School of Life Science and Technology, Zhanjiang Normal University, Zhanjiang, P.R. China
2 Hunan Agricultural University, Changsha, P.R. China

Seedling hypocotyls were used as explants to establish a regeneration protocol for Eucalyptus urophylla and N-phenyl-N'-[6-(2-chlorobenzothiazol)-yl] urea (PBU), one kind of di-substituted urea, was found useful growth regulator. The hypocotyls incubated on a modified Murashige and Skoog medium (SPCa), supplemented with 6.6 μM PBU and 0.57 μM indole-3-acetic acid (IAA) dedifferentiated and form calli (100 % after 7 d). Compared with other growth regulator combinations, PBU stimulated more vigorous calli and restrained their darkening. In addition, the calli induced by PBU showed high frequency of adventitious buds formation (57%). Shoot proliferation and elongation was then stimulated on SPCa medium containing 0.44 μM 6-benzyladenine (BA), 0.54 μM naphthalene acetic acid (NAA) and 0.3 μM gibberellic acid (GA3). For rooting, shoots were cultivated on root induction medium containing 2.5 μM indole-3-butyric acid (IBA). Plantlets were then successfully transplanted to greenhouse.

Keywords: bud induction; elongation; proliferation; substituted urea
Subjects: auxins; cytokinins; Eucalyptus urophyla; ex vitro transfer; in vitro culture, callus; in vitro culture, rooting

Received: April 5, 2008; Accepted: December 1, 2008; Published: March 1, 2010  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Huang, Z.-C., Zeng, F.-H., & Lu, X.-Y. (2010). Efficient regeneration of Eucalyptus urophylla from seedling-derived hypocotyls. Biologia plantarum54(1), 131-134. doi: 10.1007/s10535-010-0020-4
Download citation

References

  1. Arruda, S.C.C., Souza, G.M., Almeida, M., Gonçalves, A.N.: Anatomical and biochemical characterization of the calcium effect on Eucalyptus urophylla callus morphogenesis in vitro - Plant Cell Tissue Organ Cult. 63: 143-154, 2000. Go to original source...
  2. Barrueto, C.L.P., Machado C.M.G.A., Carvalheira B.R.C.S., Brasileiro, A.C.M.: Plant regeneration from seedling explants of Eucalyptus grandis × E. urophylla - Plant Cell Tissue Organ Cult. 56: 17-23, 1999. Go to original source...
  3. Carra, A., De Pasquale, F., Ricci, A., Carimi, F.: Diphenylurea derivatives induce somatic embryogenesis in Citrus - Plant Cell Tissue Organ Cult. 87: 41-48, 2006. Go to original source...
  4. Chung, H.H., Chen, J.T., Chang, W.C.: Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium. - Biol. Plant. 51: 346-350, 2007. Go to original source...
  5. Harcourt, R.L., Kyozuka, J., Floyd, R.B., Bateman, K.S., Tanaka, H., Decroocq, V., Llewellyn, D.J., Zhu, X., Peacock, W.J., Dennis, E.S.: Insect- and herbicide-resistant transgenic eucalypts. - Mol. Breed. 6: 307-315, 2000. Go to original source...
  6. Khan, H., Siddique, I., Anis, M.: Thidiazuron induced somatic embryogenesis and plant regeneration in Capsicum annuum. - Biol. Plant. 50: 789-792, 2006. Go to original source...
  7. Li, Z.F., Luo, F.Y.: Synthesis and characterization of N-substituted phenyl-N'-[6-(2-chlorobenzothiazol)-yl] urea. - Chem. Res. Appl. 13: 80-82, 2001.
  8. Ricci, A., Carra, A., Rolli, E., Bertoletti, C., Morini, G., Incerti, M., Vicini, P.: Effect of Cl-substitution on rooting or cytokinin-like activity of diphenylurea derivatives. - J. Plant Growth Regul. 23: 261-268, 2005. Go to original source...
  9. Tournier, V., Grat, S., Marque, C., Kayal, E.W., Penchel, D.R., Andrade, D.G., Boudet, A.M., Teulières, C.: An efficient procedure to stably introduce genes into an economically important pulp tree (Eucalyptus grandis × Eucalyptus urophylla) - Transgenic Res. 12: 403-411, 2003. Go to original source...
  10. Turker, A.U., Yucesan, B., Gurel, E.: An efficient in vitro regeneration system for Lythrum salicaria. - Biol. Plant. 53: 750-754, 2009. Go to original source...
  11. Yamada, H., Suzuki, T., Terada, K., Takei, K., Ishikawa, K., Miwa, K., Yamashino, T., Mizuno, T.: The Arabidopsis AHK4 histidine kinase is a cytokinin-binding receptor that transduces cytokinin signals across the membrane. - Plant Cell Physiol. 42: 1017-1023, 2001. Go to original source...