biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:635-640, 2007 | DOI: 10.1007/s10535-007-0135-4

Direct somatic embryogenesis from leaves, cotyledons and hypocotyls of Hippophae rhamnoides

C. Q. Liu1,2, X. L. Xia1, W. L. Yin1,*, J. H. Zhou3, H. R. Tang4
1 College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, P.R. China
2 Graduate School, Beijing Forestry University, Beijing, P.R. China
3 Phytopathology Key Laboratory, Yunnan Agricultural University, Kunming, Yunnan, P.R. China
4 College of Forest and Horticulture, Sichuan Agricultural University, Yaan, Sichuan, P.R. China

Plant regeneration via direct somatic embryogenesis from cotyledons, hypocotyls and leaves in seabuckthorn (Hippophae rhamnoides L.) was achieved. The influences of basal media, carbon sources, plant growth regulators (PGRs) with different concentrations and combinations on embryogenesis capacity of explants were studied. The highest frequency of somatic embryos production and germination was obtained on Schenk and Hildebrandt medium (SH) supplemented with 1.0 mg dm-3 kinetin and 0.2, 0.5 mg dm-3 indole-3-acetic acid. Granulated sugar was the optimal carbon source. The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse with a maximum survival rate of 55 %. Histological observation revealed that the somatic embryos were similar to those of zygotic embryos.

Keywords: indole-3-butyric acid; in vitro morphogenesis; kinetin; plant regeneration; seabuckthorn
Subjects: auxins; cotyledon, cotyledonary node; cytokinins; hypocotyl; Hyppophae rhamnoides; in vitro culture, embryogenesis, somatic embryogenesis; nutrient medium, Lloyd and McCown (WPM); nutrient medium, Murashige and Skoog (MS); nutrient medium, Schenk and Hildebrandt (SH); seabuckthorn; somatic embryogenesis

Received: September 12, 2005; Accepted: July 15, 2006; Published: December 1, 2007  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Liu, C.Q., Xia, X.L., Yin, W.L., Zhou, J.H., & Tang, H.R. (2007). Direct somatic embryogenesis from leaves, cotyledons and hypocotyls of Hippophae rhamnoides. Biologia plantarum, 51(4), 635-640. doi: 10.1007/s10535-007-0135-4
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abdoulaye, T. (ed.): Somatic Embryogenesis, Embryo Conversion, Micropropagation and Factors Affecting Genetic Transformation of Theobroma cacao L.-UMI Dissertation Services, Pennsylvania 2000.
    2. Akhtar, N., Kumari, N., Pandey, S., Ara, H., Singh, M., Jaiswal, U., Jaiwal, V.S., Jain, S.M.: Somatic embryogenesis in tropical fruit trees.-In: Jain, S.M., Gupta, P.K., Newton, R.J. (ed.): Somatic Embryogenesis in Woody Plants. Vol. 6. Pp. 93-140. Kluwer Academic Publishers, Dordrecht 2000. Go to original source...
    3. Daigny, G., Paual, H., Sangwan, R.S., Sangwan-Norreel, B.S.: Factors influencing secondary somatic embryogenesis in Malus × domestica Borkh. (cv. 'Gloster 69).-Plant Cell Rep. 16: 153-157, 1996. Go to original source...
    4. Ganesan, M., Jayabalan, N.: Evaluation of haemoglobin (erythrogen): for improved somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L. cv. SVPR2).-Plant Cell Rep. 23: 181-187, 2004. Go to original source...
    5. Hassanein, A.M.: Somatic embryogenesis of cucumber (Cucumis sativus L.) using seed cuttings obtained from premature fruit.-Plant Biotechnol. 20: 275-281, 2003. Go to original source...
    6. Knyazev, A.V., Chemeris, A.V., Vakhitov, V.A.: [Morphogenesis of Hippophae rhamnoides L. in vitro.]-Rast. Resurs. 39: 107-115, 2003. [In Russ.]
    7. Ladyman, J.A.R., Girard, B.: Cucumber somatic development on various gelling agents and carbohydrates sources.-Hort. Sci. 27: 164-165, 1992. Go to original source...
    8. Li, H.Q., Liang, C.Y., Huang, Y.W., Guo, J.Y.: [Factors influencing the developments of secondary embryoids and plant regeneration on cassava (Manihot esculenta Crantz).]-Guihaia 19: 246-250, 1999. [In Chin.]
    9. Litz, R.E., Gray, D.J.: Organogenesis and somatic embryogenesis.-In: Hammerschlag, F.A., Litz, R.E. (ed.): Biotechnology of Perennial Fruit Crops. Pp. 3-34. University Press, Cambridge 1992.
    10. Lloyd, G., McCown, B.: Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture.-Proc. Int. Plant Propag. Soc. 30: 421-427, 1980.
    11. Lu, S.G., Bao, W.L., Meng, X.T.: Exploitation and ultilization of seabuckthorn in China.-In: Worldwide Research and Development of Seabuckthorn: Proceeding of International Workshop on Seabuckthorn, 1995. Pp. 1-8. China Sci-Tech Press, Beijing 1997.
    12. Merkie, S.A., Parrott, W.A., Flinn, B.S.: Morphogenic aspects of somatic embryogenesis.-In: Thorpe, T.A. (ed.): In Vitro Embryogenesis in Plant. Vol. 1. Pp. 3-8. Kluwer Academic Publishers, Dordrecht 1995. Go to original source...
    13. Montpetit, D., Lalonde, M.: In vitro propagation and subsequent nodulation of the actinorhizal Hippophae rhamnoides L.-Plant Cell Tissue Organ Cult. 15: 189-199, 1988. Go to original source...
    14. Mou, Y.Y.: Micropropagation of seabuckthorn (Hippophae rhamnoides L.).-Agr. Sci. Finland 4: 503-512, 1995. Go to original source...
    15. Murashige, T., Skoog, F.: A revised medium for rapid growth and bio-assays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    16. Nitsch, J.P., Nitsch, C.: Haploid plants from pollen grains.-Science 163: 85-87, 1969. Go to original source...
    17. Palacios, A.M., Larrocea, M.P.O., Chávez, V.M., Bye, R.: Somatic embryogenesis of Agave victoriaereginae: considerations for its conservation.-Plant Cell Tissue Organ Cult. 74: 135-142, 2003. Go to original source...
    18. Parrot, W.A., Bailey, M.A.: Characterization of recurrent somatic embryogenesis of alfalfa on auxin-free medium.-Plant Cell Tissue Organ Cult. 32: 69-76, 1993. Go to original source...
    19. Raj, B.R.: Micropragation of temperate and tropical fruit trees through repetitive somatic embryogenesis.-In: XII Plant Tissue Culture Conference. Pp. 16. North-Eastern Hill University Press, Shillong 1989.
    20. Schenk, R.U., Hildebrandt, A.C.: Medium and techniques for induction and growth of monocotyledons and dicotyledonous plant cell cultures.-Can. J. Bot. 50: 199-204, 1972. Go to original source...
    21. Soh, W.Y.: Germinability and cotyledon structure of somatic embryos in some dicotyledonous plants.-In: Xu, Z.H., Chen, Z.H. (ed.): Plant Biotechnology for Sustainable Development of Agriculture. Pp. 51-59. China Forestry Publishing House, Beijing 1996.
    22. Strickland, S.G., Nichol, J.W., McCall, C.M., Stuart, A.D.: Effect of carbohydrate source on alfalfa somatic embryogenesis.-Plant Sci. 48: 113-121, 1987. Go to original source...
    23. Sun, Y.Q., Zhang, X.L., Jin, S.X., Liang, S.G., Nie, Y.C.: Somatic embryogenesis and plant regeneration in wild cotton (Gossypium klotzschianum).-Plant Cell Tissue Organ Cult. 75: 247-253, 2003. Go to original source...
    24. Vieitez, E., Ballester, A., Vieitez, A.M.: Somatic embryogenesis and plant regeneration from cell suspension cultures of Fagus sylvatica L..-Hort. Rev. 14: 265-332, 1992. Go to original source...
    25. Vooková, B., Kormut'ák, A.: Effect of sucrose concentration, charcoal, and indole-3-butyric acid on germination of Abies numidica somatic embryos.-Biol. Plant. 44: 181-184, 2001. Go to original source...
    26. Yang, H.P., Chen, J.C., Kamada, H.: Multiple pathways of somatic embryogenesis at a higher frequency in Asparagus officinalis L..-Plant Biotechnol. 17: 111-118, 2000. Go to original source...
    27. Yang, L.P., Zhang, F.L., Zhao, X.M.: [Rapid propagation of seabuckthorn (Hippophae rhamnoides L.).]-Global Seabuckthorn Res. Dev. 2: 12-16, 2004. [In Chin.]

    Return to the content