Abstract
Mycosynthesis of silver nano-scale particles by fungi is an important aspect in nanotechnology due to its eco-friendly, safe, and cost-effective nature. It also provides protein-capped nanoparticles, which are very stable and have good dispersion. The Mycosynthesis of silver nano-scale particles (SNPs) by Trichoderma asperellum Filtrate (TAF) was evaluated. The characterizations of TAFSNPs were done by UV–visible spectroscopy, FTIR, SEM, and EDX. Biological evaluations such as antibacterial activity against some pathogens, in-vivo immuno-stimulatory, and antitumor potential and hematological parameters of SNPs of T. asperellum were investigated. Changes in color from yellow to brown indicate the formation of nanoparticles. TAFSNPs biosynthesis had a Surface Plasmon Resonance peak at 450 nm. The nanoparticles were spherical, with a size ranging from 0.7 to 10.0 nm. EDX analysis revealed a strong signal for the silver element. TAFSNPs had profound antagonistic activity against the test microorganism. B. subtilis, S. aureus, and Salmonella typhi were highly susceptible with inhibition zone of 24.3 mm, 21.8 mm, and 21.3 mm. TAFSNPs had significant immune-stimulation in the treated mice with an increase in IgA and IgM production, which ranged from 169 0.83d–269 0.56a mg/dl and 26 0.16d–69 0.27a mg/dl. TAFSNPs had CEA level reduction capability (3.62–4.05 ng/L). TAFSNPs had the highest amounts of PCV, Red blood cells, heamoglobin, and neutrophils (38%, 5.7 × 1012%, 14.09 g/dl, and 71%). Significant improvement was recorded on the lifespan of the TAFSNPs treated tumor-induced mice. Conclusively, TAFSNPs have good antimicrobial, immuno-stimulatory, and antitumor potential.
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This work was carried out in collaboration between all authors. Author BCAT: designed and wrote the protocol the study. Author GEO: wrote the first draft of the manuscript and managed the analyses. Authors GEO and KAO: managed the literature searches and performed the statistical analysis. All authors read and approved the final manuscripts.
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Ogunleye, G.E., Adebayo-Tayo, B.C. & Oyinlola, K.A. Biological evaluation of extracellular mycosynthesized silver nanoparticles by Trichoderma asperellum. Biometals 36, 97–109 (2023). https://doi.org/10.1007/s10534-022-00463-9
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DOI: https://doi.org/10.1007/s10534-022-00463-9