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Selenoprotein W gene expression in the gastrointestinal tract of chicken is affected by dietary selenium

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Abstract

Selenoprotein W (SelW) and selenium (Se) plays important roles in gastrointestinal function and that SelW expression in the gastrointestinal system of mammals is sensitive to Se levels. However, little is known about the pattern of SelW expression in the bird gastrointestinal tract. To investigate the distribution of SelW and effects of dietary Se levels on the SelW mRNA expression in the gastrointestinal tract tissues of birds, 1-day-old male chickens were fed either a commercial diet or a Se-supplemented diet containing 1.0, 2.0, 3.0 or 5.0 mg/kg sodium selenite for 90 days. The gastrointestinal tract tissues (tongue, esophagus, crop, proventriculus, gizzard, duodenum, small intestine, cecum and rectum) were collected and examined for Se content and mRNA levels of SelW. The mRNA expression of SelW was detected in all tissues. The greatest increase in SelW mRNA levels was observed in the gizzard, whereas Se content was highest in the duodenum and small intestine. A significant increase in SelW mRNA levels was observed in the gastrointestinal tract tissues of chickens fed the diets containing 1–3 mg/kg sodium selenite while decreased SelW mRNA levels were observed in the esophagus, crop, proventriculus, gizzard, duodenum and cecum in chickens fed the diet containing 5 mg/kg sodium selenite. These data indicate that SelW is widely expressed in the gastrointestinal tract tissues of birds and the transcription of the SelW gene is very sensitive to dietary Se.

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References

  • Aachmann FL, Fomenko DE, Soragni A, Gladyshev VN, Dikiy A (2007) Solution structure of selenoprotein W and NMR analysis of its interaction with 14-3-3 proteins. J Biol Chem 282(51):37036–37044

    Article  PubMed  CAS  Google Scholar 

  • Beilstein MA, Vendeland SC, Barofsky E, Jensen ON, Whanger PD (1996) Selenoprotein W of rat muscle binds glutathione and an unknown small molecular weight moiety. J Inorg Biochem 61(2):117–124

    Article  PubMed  CAS  Google Scholar 

  • Bellingham J, Gregory-Evans K, Fox MF, Gregory-Evans CY (2003) Gene structure and tissue expression of human selenoprotein W, SEPW1, and identification of a retroprocessed pseudogene, SEPW1P. Biochim Biophys Acta 1627(2–3):140–146

    PubMed  CAS  Google Scholar 

  • Berry MJ (2005) Insights into the hierarchy of selenium incorporation. Nat Genet 37(11):1162–1163

    Article  PubMed  CAS  Google Scholar 

  • Brown KM, Arthur JR (2001) Selenium, selenoproteins and human health: a review. Public Health Nutr 4(2B):593–599

    Article  PubMed  CAS  Google Scholar 

  • Castellano S, Lobanov AV, Chapple C, Novoselov SV, Albrecht M, Hua D et al (2005) Diversity and functional plasticity of eukaryotic selenoproteins: identification and characterization of the SelJ family. Proc Natl Acad Sci USA 102(45):16188–16193

    Article  PubMed  CAS  Google Scholar 

  • Chariot P, Bignani O (2003) Skeletal muscle disorders associated with selenium deficiency in humans. Muscle Nerve 27(6):662–668

    Article  PubMed  CAS  Google Scholar 

  • Combs GF Jr (2005) Current evidence and research needs to support a health claim for selenium and cancer prevention. J Nutr 135(2):343–347

    PubMed  CAS  Google Scholar 

  • Combs GF Jr, Clark LC, Turnbull BW (2001) An analysis of cancer prevention by selenium. Biofactors 14(1–4):153–159

    Article  PubMed  CAS  Google Scholar 

  • Gu QP, Beilstein MA, Barofsky E, Ream W, Whanger PD (1999) Purification, characterization, and glutathione binding to selenoprotein W from monkey muscle. Arch Biochem Biophys 361(1):25–33

    Article  PubMed  CAS  Google Scholar 

  • Gu QP, Sun Y, Ream LW, Whanger PD (2000) Selenoprotein W accumulates primarily in primate skeletal muscle, heart, brain and tongue. Mol Cell Biochem 204(1–2):49–56

    Article  PubMed  CAS  Google Scholar 

  • Gu QP, Ream W, Whanger PD (2002) Selenoprotein W gene regulation by selenium in L8 cells. Biometals 15(4):411–420

    Article  PubMed  CAS  Google Scholar 

  • Hasunuma R, Ogawa T, Kawanishi Y (1982) Fluorometric determination of selenium in nanogram amounts in biological materials using 2,3-diaminonaphthalene. Anal Biochem 126(2):242–245

    Article  PubMed  CAS  Google Scholar 

  • Hoffmann PR, Berry MJ (2008) The influence of selenium on immune responses. Mol Nutr Food Res 52(11):1273–1280

    Article  PubMed  CAS  Google Scholar 

  • Hoffmann PR, Hoge SC, Li PA, Hoffmann FW, Hashimoto AC, Berry MJ (2007) The selenoproteome exhibits widely varying, tissue-specific dependence on selenoprotein P for selenium supply. Nucleic Acids Res 35(12):3963–3973

    Article  PubMed  CAS  Google Scholar 

  • Jeong D, Kim TS, Chung YW, Lee BJ, Kim IY (2002) Selenoprotein W is a glutathione-dependent antioxidant in vivo. FEBS Lett 517(1–3):225–228

    Article  PubMed  CAS  Google Scholar 

  • Kaur P, Bansal MP (2005) Effect of selenium-induced oxidative stress on the cell kinetics in testis and reproductive ability of male mice. Nutrition 21(3):351–357

    Article  PubMed  CAS  Google Scholar 

  • Kryukov GV, Gladyshev VN (2004) The prokaryotic selenoproteome. EMBO Rep 5(5):538–543

    Article  PubMed  CAS  Google Scholar 

  • Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guigo R et al (2003) Characterization of mammalian selenoproteomes. Science 300(5624):1439–1443

    Article  PubMed  CAS  Google Scholar 

  • Li JL, Gao R, Li S, Wang JT, Tang ZX, Xu SW (2010) Testicular toxicity induced by dietary cadmium in cocks and ameliorative effect by selenium. Biometals 23(4):695–705

    Article  PubMed  CAS  Google Scholar 

  • Lu J, Holmgren A (2009) Selenoproteins. J Biol Chem 284(2):723–727

    Article  PubMed  CAS  Google Scholar 

  • Mahmoud KZ, Edens FW (2005) Influence of organic selenium on hsp70 response of heat-stressed and enteropathogenic Escherichia coli-challenged broiler chickens (Gallus gallus). Comp Biochem Physiol C 141(1):69–75

    Google Scholar 

  • Martin-Romero FJ, Kryukov GV, Lobanov AV, Carlson BA, Lee BJ, Gladyshev VN et al (2001) Selenium metabolism in Drosophila: selenoproteins, selenoprotein mRNA expression, fertility, and mortality. J Biol Chem 276(32):29798–29804

    Article  PubMed  CAS  Google Scholar 

  • Moriarty PM, Reddy CC, Maquat LE (1998) Selenium deficiency reduces the abundance of mRNA for Se-dependent glutathione peroxidase 1 by a UGA-dependent mechanism likely to be nonsense codon-mediated decay of cytoplasmic mRNA. Mol Cell Biol 18(5):2932–2939

    PubMed  CAS  Google Scholar 

  • Pagmantidis V, Bermano G, Villette S, Broom I, Arthur J, Hesketh J (2005) Effects of Se-depletion on glutathione peroxidase and selenoprotein W gene expression in the colon. FEBS Lett 579(3):792–796

    Article  PubMed  CAS  Google Scholar 

  • Papp LV, Lu J, Holmgren A, Khanna KK (2007) From selenium to selenoproteins: synthesis, identity, and their role in human health. Antioxid Redox Signal 9(7):775–806

    Article  PubMed  CAS  Google Scholar 

  • Peirson SN, Butler JN, Foster RG (2003) Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Nucleic Acids Res 31(14):e73

    Article  PubMed  Google Scholar 

  • Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29(9):e45

    Article  PubMed  CAS  Google Scholar 

  • Rayman MP (2000) The importance of selenium to human health. Lancet 356(9225):233–241

    Article  PubMed  CAS  Google Scholar 

  • Reeves MA, Hoffmann PR (2009) The human selenoproteome: recent insights into functions and regulation. Cell Mol Life Sci 66(15):2457–2478

    Article  PubMed  CAS  Google Scholar 

  • Schweizer U, Schomburg L, Savaskan NE (2004) The neurobiology of selenium: lessons from transgenic mice. J Nutr 134(4):707–710

    PubMed  CAS  Google Scholar 

  • Stadtman TC (2000) Selenium biochemistry. Mammalian selenoenzymes. Ann N Y Acad Sci 899:399–402

    Article  PubMed  CAS  Google Scholar 

  • Sun Y, Ha PC, Butler JA, Qu BR, Yeh JY, Whanger PD (1998) Effect of dietary selenium on selenoprotein W and glutathione peroxidase in 28 tissues of the rat. J Nutr Biochem 9:23–27

    Article  CAS  Google Scholar 

  • Sun Y, Butler J, Forsberg N, Whanger PD (1999) Selenoprotein W, selenium and glutathione peroxidase in rat and sheep brains and in brain cell cultures. Nutr Neurosci 2:227–237

    Google Scholar 

  • Sunde RA, Raines AM, Barnes KM, Evenson JK (2009) Selenium status highly regulates selenoprotein mRNA levels for only a subset of the selenoproteins in the selenoproteome. Biosci Rep 29(5):329–338

    Article  PubMed  CAS  Google Scholar 

  • Terry EN, Michal JJ, Hostetler CE, Kincaid RL, Johnson KA (2009) Levels of mRNA for three selenoproteins in skeletal muscle of fetal and newborn pigs. Livest Sci 124:21–25

    Article  Google Scholar 

  • Vendeland SC, Beilstein MA, Yeh JY, Ream W, Whanger PD (1995) Rat skeletal muscle selenoprotein W: cDNA clone and mRNA modulation by dietary selenium. Proc Natl Acad Sci USA 92(19):8749–8753

    Article  PubMed  CAS  Google Scholar 

  • Wang XL, Yang CP, Xu K, Qin OJ (2010) Selenoprotein W depletion in vitro might indicate that its main function is not as an antioxidative enzyme. Biochemistry 75(2):201–207

    CAS  Google Scholar 

  • Whanger PD (2000) Selenoprotein W: a review. Cell Mol Life Sci 57(13–14):1846–1852

    Article  PubMed  CAS  Google Scholar 

  • Yeh JY, Beilstein MA, Andrews JS, Whanger PD (1995) Tissue distribution and influence of selenium status on levels of selenoprotein W. FASEB J 9(5):392–396

    PubMed  CAS  Google Scholar 

  • Yeh JY, Gu QP, Beilstein MA, Forsberg NE, Whanger PD (1997a) Selenium influences tissue levels of selenoprotein W in sheep. J Nutr 127(3):394–402

    PubMed  CAS  Google Scholar 

  • Yeh JY, Vendeland SC, Gu Q, Butler JA, Ou BR, Whanger PD (1997b) Dietary selenium increases selenoprotein W levels in rat tissues. J Nutr 127(11):2165–2172

    PubMed  CAS  Google Scholar 

  • Yeh JY, Ou BR, Gu QP, Whanger PD (1998) Influence of gender on selenoprotein W, glutathione peroxidase and selenium in tissues of rats. Comp Biochem Physiol B 119(1):151–155

    Article  PubMed  CAS  Google Scholar 

  • Zhang Y, Fomenko DE, Gladyshev VN (2005) The microbial selenoproteome of the Sargasso Sea. Genome Biol 6(4):R37

    Article  PubMed  Google Scholar 

  • Zhou JC, Zhao H, Li JG, Xia XJ, Wang KN, Zhang YJ et al (2009) Selenoprotein gene expression in thyroid and pituitary of young pigs is not affected by dietary selenium deficiency or excess. J Nutr 139(6):1061–1066

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgment

This study was supported by the National Natural Science Foundation of China (30871902).

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Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Jin-Long Li, Shu Li, Zhi-Hui Jiang & Shi-Wen Xu

  2. College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510640, People’s Republic of China

    Jin-Long Li & Zhao-Xin Tang

  3. Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, People’s Republic of China

    Hui-Xin Li

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  1. Jin-Long Li
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  2. Hui-Xin Li
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  3. Shu Li
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  4. Zhi-Hui Jiang
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  5. Shi-Wen Xu
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  6. Zhao-Xin Tang
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Correspondence to Shi-Wen Xu or Zhao-Xin Tang.

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Jin-Long Li and Hui-Xin Li contributed equally to this work.

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Li, JL., Li, HX., Li, S. et al. Selenoprotein W gene expression in the gastrointestinal tract of chicken is affected by dietary selenium. Biometals 24, 291–299 (2011). https://doi.org/10.1007/s10534-010-9395-0

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