Abstract
A hydroponic experiment was carried out to characterize the oxidative stress responses of two potato cultivars (Solanum tuberosum L. cvs. Asterix and Macaca) to cadmium (Cd). Plantlets were exposed to four Cd levels (0, 50, 100, 150 and 200 μM) for 7 days. Cd concentration was increased in both roots and shoot. Number of sprouts and roots was not decreased, whereas Cd treatment affected the number of nodal segments. Chlorophyll content and ALA-D activity were decreased in both cultivars, whereas carotenoids content was decreased only in Macaca. Cd caused lipid peroxidation in roots and shoot of both cultivars. Protein oxidation was only verified at the highest Cd level. H2O2 content was increased in roots and shoot of Asterix, and apparently, a compensatory response between roots and shoot of Macaca was observed. SOD activity was inhibited in roots of Asterix at all Cd treatments, whereas in Macaca it was only increased at two highest Cd levels. Shoot SOD activity increased in Asterix and decreased in Macaca. Root CAT activity in Asterix decreased at 100 and 150 μM, whereas in Macaca it decreased only at 50 μM. Shoot CAT activity was decreased in Macaca. Root AsA content in Macaca was not affected, whereas in shoot it was reduced at 100 μM and increased at 200 μM. Cd caused increase in NPSH content in roots and shoot. Our results suggest that Cd induces oxidative stress in both potato cultivars and that of the two cultivars, Asterix showed greater sensitivity to Cd levels.
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References
Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126. doi:10.1016/S0076-6879(84)05016-3
Arvind P, Prasad MNV (2005) Cadmium–zinc interactions in a hydroponic system using Ceratophyllum demersum L.: adaptative ecophysiology, biochemistry and molecular toxicology. Braz J Plant Physiol 17:3–20
Barceló J, Poschenrieder C (1990) Plant water relations as affected by heavy metal stress: a review. J Plant Nutr 13:1–37. doi:10.1080/01904169009364057
Behera RK, Mishra PC, Choudhury NK (2002) High irradiance and water stress induce alterations in pigment composition and chloroplast activities of primary wheat leaves. J Plant Physiol 159:967–973. doi:10.1078/0176-1617-00823
Benavides MP, Gallego SM, Tomaro ML (2005) Cadmium toxicity in plants. Braz J Plant Physiol 17:21–34. doi:10.1590/S1677-04202005000100003
Berlett BS, Stadman ER (1997) Protein oxidation in aging, disease and oxidative stress. J Biol Chem 272:20313–20316. doi:10.1074/jbc.272.33.20313
Bienert GP, Schjoerring JK, Jahn TP (2006) Membrane transport of hydrogen peroxide. Biochim Biophys Acta 1758:994–1003. doi:10.1016/j.bbamem.2006.02.015
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantity of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. doi:10.1016/0003-2697(76)90527-3
Chen Z, Gallie DR (2004) The ascorbic acid redox state controls guard cell signaling and stomatal movement. Plant Cell 16:1143–1162. doi:10.1105/tpc.021584
Drazkiewicz M, Baszynski T (2005) Growth parameters and photosynthetic pigments in leaf segments of Zea mays exposed to cadmium, as related to protection mechanisms. J Plant Physiol 162:1013–1021
Dunbar KR, McLaughlin MJ, Reid RJ (2003) The uptake and partitioning of cadmium in two cultivars of potato (Solanum tuberosum L.). J Exp Bot 54:349–354. doi:10.1093/jxb/54.381.349
Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77. doi:10.1016/0003-9861(59)90090-6
El-Moshaty FIB, Pike SM, Novacky AJ et al (1993) Lipid peroxidation and superoxide production in cowpea (Vigna unguiculata) leaves infected with tobacco ringspot virus or southern bean mosaic virus. Physiol Mol Plant Pathol 43:109–119. doi:10.1006/pmpp.1993.1044
FAO (2007) Agricultural statistics. http://www.fao.org/statistics
Fatima RA, Ahmad M (2004) Certain antioxidant enzymes of Allium cepa as biomarkers for the detection of toxic heavy metals in wastewater. Sci Total Environ 346:256–273. doi:10.1016/j.scitotenv.2004.12.004
Frank HA, Cogdell RJ (1996) Carotenoids in photosynthesis. Photochem Photobiol 63:257–264. doi:10.1111/j.1751-1097.1996.tb03022.x
Gomes-Júnior RA, Moldes CA, Delite FS et al (2006) Antioxidant metabolism of coffee cell suspension cultures in response to cadmium. Chemosphere 65:1330–1337. doi:10.1016/j.chemosphere.2006.04.056
Gratão PL, Polle A, Lea PJ et al (2005) Making the life of heavy-metal stressed plants a little easier. Funct Plant Biol 32:481–494. doi:10.1071/FP05016
Hernández LE, Lozano-Rodríguez E, Gárate A et al (1998) Influence of cadmium on the uptake, tissue accumulation and subcellular distribution of manganese in pea seedlings. Plant Sci 132:139–151. doi:10.1016/S0168-9452(98)00011-9
Hiscox JD, Israelstam GF (1979) A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1132–1334. doi:10.1139/b79-163
Jacques-Silva MC, Nogueira CW, Broch LC et al (2001) Diphenyl diselenide and ascorbic acid changes deposition of selenium and ascorbic acid in liver and brain of mice. Pharmacol Toxicol 88:119–125. doi:10.1034/j.1600-0773.2001.d01-92.x
Kabata-Pendias A, Pendias H (2001) Trace elements in soils and plants. CRC Press Inc., Boca Raton, p 148
Levine RL, Garland D, Oliver CN et al (1990) Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 186:464–478. doi:10.1016/0076-6879(90)86141-H
Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148:350–382. doi:10.1016/0076-6879(87)48036-1
Liu J, Qian M, Cai G et al (2007) Uptake and translocation of Cd in different rice cultivars and the relation with Cd accumulation in rice grain. J Hazard Mater 143:443–447. doi:10.1016/j.jhazmat.2006.09.057
Loreto F, Velikova V (2001) Isoprene produced by leaves protects the photosynthetic apparatus against ozone damage, quences ozone products, and reduces lipid peroxidation of cellular membranes. Plant Physiol 127:1781–1787. doi:10.1104/pp.010497
Mishra S, Srivastava S, Tripathi RD et al (2006) Phytochelatin synthesis and response of antioxidants during cadmium stress in Bacopa monnieri L. Plant Physiol Biochem 44:25–37. doi:10.1016/j.plaphy.2006.01.007
Misra HP, Fridovich I (1972) The role of superoxide anion in the autoxidation of epinephrine and simple assay for superoxide dismutase. J Biol Chem 244:6049–6055
Morsch VM, Schetinger MRC, Martins AF et al (2002) Effects of cadmium, lead, mercury and zinc on δ-aminolevulinic acid dehydratase activity from radish leaves. Biol Plant 45:85–89. doi:10.1023/A:1015196423320
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiol 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x
Noctor G, Foyer CH (1998) Ascorbate and gluthatione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279. doi:10.1146/annurev.arplant.49.1.249
Noriega GO, Balestrasse KB, Batlle A et al (2007) Cadmium induced oxidative stress in soybean plants also by the accumulation of δ-aminolevulinic acid. Biometals 20:841–851. doi:10.1007/s10534-006-9077-0
Ogawa K, Kanematsu S, Asada K (1997) Generation of superoxide anion and localization of Cu–Zn superoxide dismutase in the vascular tissue of spinach hypocotyls their association with lignification. Plant Cell Physiol 38:1118–1126
Ortega-Villasante C, Rellán-Álvarez R, Del Campo FF et al (2005) Cellular damage induced by cadmium and mercury in Medicago sativa. J Exp Bot 56:2239–2251. doi:10.1093/jxb/eri223
Rascio N, Dalla Vecchia F, La Roccaa N et al (2008) Metal accumulation and damage in rice (cv. Vilone Nano) seedlings exposed to cadmium. Environ Exp Bot 62:267–278. doi:10.1016/j.envexpbot.2007.09.002
Reinheckel T, Noack H, Lorenz S et al (1998) Comparison of protein oxidation and aldehyde formation during oxidative stress in isolated mitochondria. Free Radic Res 29:297–305. doi:10.1080/10715769800300331
Rellán-álvarez R, Ortega-Villasante C, Álvarez-Fernández A et al (2006) Stress responses of Zea mays to cadmium and mercury. Plant Soil 279:41–50. doi:10.1007/s11104-005-3900-1
Romero-Puertas MC, Corpas FJ, Rodríguez-Serrano M et al (2007) Differential expression and regulation of antioxidative enzymes by cadmium in pea plants. J Plant Physiol 164:1346–1357. doi:10.1016/j.jplph.2006.06.018
Sassa S (1982) δ-Aminolevulinic acid dehydratase assay. Enzyme 28:133–145
Senesi GS, Baldassarre G, Senesi N et al (1999) Trace element inputs into soils by anthropogenic activities and implications for human health. Chemosphere 39:343–377. doi:10.1016/S0045-6535(99)00115-0
Singh S, Eapen S, D’Souza SF (2006) Cadmium accumulation and its influence on lipid peroxidation and antioxidative system in an aquatic plant, Bacopa monnieri L. Chemosphere 62:233–246. doi:10.1016/j.chemosphere.2005.05.017
Soares CRFS, Siqueira JO, De Carvalho JG et al (2005) Fitotoxidez de cádmio para Eucalypus maculata e E. urophylla em solução nutritiva. Rev Arvore 29:175–183 in Portuguese
Sohal RS, Mockett RJ, Orr WC (2002) Mechanisms of aging: an appraisal of the oxidative stress hypothesis. Free Rad Biol Med 33:575–586
Stenhouse F (1992) The 1992 Australian market basket survey. National Food Authority. Australian Government Publishing Service, Canberra
Stroinski A, Kozlowska M (1997) Cadmium-induced oxidative stress in potato tuber. Acta Soc Bot Pol 66:189–195
Tennant D (1975) A test of a modified line intersect method of estimating root length. J Ecol 63:995–1001. doi:10.2307/2258617
Tiryakioglu M, Eker S, Ozkutlu F et al (2006) Antioxidant defense system and cadmium uptake in barley genotypes differing in cadmium tolerance. J Trace Elem Med Biol 20:181–189. doi:10.1016/j.jtemb.2005.12.004
Verma S, Dubey RS (2003) Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci 164:645–655. doi:10.1016/S0168-9452(03)00022-0
Vitória AP, Lea PJ, Azevedo AA (2001) Antioxidant enzymes responses to cadmium in radish tissues. Phytochemistry 57:701–710
Xiang C, Oliver DJ (1998) Glutathione metabolic genes coordinately respond to heavy metals and jasmonic acid in Arabidopsis. Plant Cell 10:1539–1550
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The authors thank to Coordenação e Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação de Amparo à Pesquisa de Estado do Rio Grande do Sul (FAPERGS) for the researches fellowships.
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Gonçalves, J.F., Tabaldi, L.A., Cargnelutti, D. et al. Cadmium-induced oxidative stress in two potato cultivars. Biometals 22, 779–792 (2009). https://doi.org/10.1007/s10534-009-9225-4
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DOI: https://doi.org/10.1007/s10534-009-9225-4