Abstract
Plant litter decomposition is a key ecological process that is mostly studied at the forest floor. However, decomposition generally starts in the canopy. In this study, we evaluated the effect of litter composition and climate on the initial phase of decomposition in the soil and two contrasting types of canopy microsites along an elevational gradient (0–2200 m a.s.l.). To this end, we incubated standard material composed by green (fast decomposing) and rooibos (slow decomposing) tea bags for three months. Tea bags were placed in soil (buried at 5 cm) and in the canopy at ca. 5 m above the ground in “micro-wetlands” (tank bromeliads) and dry crown microsites (branches). Along the elevational gradient, green tea decomposed faster than rooibos tea in all microsites and forests. Mass loss for both tea types was lowest on branches at all sites, except for green tea in a wet forest where decomposition did not significantly differ among microsites. In wet forests, decomposition did not differ between bromeliads and soil, while in a dry forest, decomposition was faster in bromeliads. We found that the effects of climatic variables [monthly average temperature (TEMP) and total precipitation (PREC) for the incubation months] on decomposition differed between microsites. Along the elevational gradient, the mass loss in soil was positively correlated with TEMP but not with PREC, whereas on branches, mass loss was negatively correlated with TEMP and positively correlated with PREC. Unlike on branches, mass loss in bromeliads slightly decreased with PREC and increased with TEMP. Our study shows that microsite conditions interact with climate (TEMP and PREC) leading to differences in the general decomposition patterns in the forest canopy.
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Introduction
Plant litter decomposition is a key ecological process that involves a series of physical, chemical, and biological processes that transform organic matter into increasingly stable forms, contributing to soil fertility, long-term carbon storage, and nutrient regeneration (Berg and McClaugherty 2014). This process is commonly divided in two stages: an early phase, stimulated by high levels of nutrients such as N, P and S in which water soluble substances and non-lignified cellulose and hemicellulose are leached and decomposed, and a late phase, when mainly lignified materials remain and litter degradation is dominated by lignin mass loss (Ibrahima et al. 1995; Coûteaux et al. 1995; Berg and McClaugherty 2014). Although plant litter decomposition primarily takes place on the forest floor, degradation of aboveground plant material generally begins in the canopy (Fonte and Schowalter 2004).
In a forest canopy, tree leaves may be colonized by pioneer decomposer organisms that start decomposition even before abscission (Šnajdr et al. 2011; Voříšková and Baldrian 2013). Dying branches also start to decay before they fall and may lose nearly 40% of their original mass in the canopy (Swift et al. 1976). Furthermore, in many temperate, subtropical and tropical forests dead epiphytes, decaying bark, and tree litter are trapped in stable locations in the canopy where they stay long enough to rot and form arboreal soil (Delamare-Deboutteville 1948; Nadkarni 1984; Hofstede et al. 2001; Fonte and Schowalter 2004; Enloe et al. 2006; Díaz et al. 2010). This phenomenon is particularly important in forests where the atmospheric humidity is high and epiphytes and litter-trapping plants (sensu Zona and Christenhusz 2015) are abundant, e.g. tropical montane forests, tropical rain forests and temperate rain forests (Sugden and Robins 1979; Nadkarni 1984; Álvarez-Sánchez and Guevara 1999; Hofstede et al. 2001; Díaz et al. 2010). In such ecosystems litter-trapping plants can intercept up to 50% of total litterfall (Álvarez-Sánchez and Guevara 1999; Dearden and Wardle 2008), and together with epiphytes, this trapped litter provides enough source material to develop thick layers of arboreal soil (Nadkarni 1984; Hofstede et al. 2001; Díaz et al. 2010). For example, in an epiphyte-rich forest in Chile, epiphyte biomass may add 40–150% of photosynthetic biomass to the tree crowns, and litter trapping bromeliads enhance organic matter deposition and retention in the canopy, accumulating more than 50% of the total arboreal soil (Díaz et al. 2010).
This arboreal soil (AS) formed and accumulated in the canopy can be defined as a Histosol (Nadkarni et al. 2002; Enloe et al. 2006) in which, as in terrestrial soils, pedogenic processes operate over time e.g. organic matter decomposition and nutrient mineralization (Vance and Nadkarni 1990; Bermudes and Benzing 1991; Paoletti et al. 1991; Inselsbacher et al. 2007). Furthermore, AS is a rich habitat for invertebrates and microbes which are common in the pedosphere (Nadkarni and Longino 1990; Paoletti et al. 1991; Fragoso and Rojas-Fernández 1996; Lindo and Winchester 2006). Considering that AS and terrestrial soil share general processes (decomposition and mineralization of nutrients) and functional groups of biota, decomposition in the canopy should be controlled by the same three essential factors that control decomposition in the terrestrial soil: (1) climate (Berg et al. 1993; Aerts 1997; Bradford et al. 2016); (2) litter chemical composition (Aerts 1997; Cornwell et al. 2008; Zhang et al. 2008), and (3) biota (Seastedt 1984; Verhoef and Brussaard 1990; Wall et al. 2008).
However, information on decomposition in the canopy is still scarce, and observations are inconsistent. Some studies have found that decay in the canopy is slower than in terrestrial soil when litter is incubated on branches and limb junctions (Nadkarni and Matelson 1991; Clark et al. 1998; Lindo and Winchester 2007; Li et al. 2014), while others report that decomposition can be as fast or even faster than in the pedosphere when this process takes place in the canopy of understory plants or epiphytes such as tank bromeliads (Paoletti et al. 1991; Dearden and Wardle 2008). This discrepancy may be the result of differences in biotic and abiotic conditions occurring at a fine spatial scale. Branches are dry crown microsites (Zotz and Hietz 2001; Stuntz et al. 2002) where the environmental conditions can be harsh for decomposers, whereas epiphytes can ameliorate environmental challenges (Stuntz et al. 2002; Scheffers et al. 2014; Zotz et al. 2020), and increase the density and diversity of decomposer (Paoletti et al. 1991; Pittl et al. 2010). Consequently, different decomposition patterns in the canopy can emerge at different microsites, and climatic controls at a regional scale can be only a surrogate for differences in microclimate (Bradford et al. 2016, 2017).
Canopy decomposition studies are typically not standardized. Although most studies use the litterbag technique, protocols differ substantially in regard to litter type, incubation time, and bag characteristics such as size and mesh opening (Paoletti et al. 1991; Clark et al. 1998; Lindo and Winchester 2007; Dearden and Wardle 2008; Cardelús 2010; Prescott 2010; Li et al. 2014; LeCraw et al. 2017). As a result, studies are only partially comparable as is the identification of common drivers of litter decomposition using such heterogeneous data (Didion et al. 2016; Djukic et al. 2018). Recently, Keuskamp et al. (2013) proposed the use of two types of commercially available tea bags (green and rooibos) as standard substrates to study the effect of litter composition and environmental factors on litter decomposition across sites and ecosystems (Keuskamp et al. 2013; Didion et al. 2016; Djukic et al. 2018; Petraglia et al. 2019). These two tea types differ chemically: Green tea has more water-soluble substances and higher cellulose content and is expected to decompose faster, while rooibos tea with its high non-soluble compounds and lignin should experience slower decomposition (Keuskamp et al. 2013; Didion et al. 2016; Djukic et al. 2018).
In this study, we used these standard tea bags to study litter decomposition in the canopy and in terrestrial soil along an elevational gradient in Veracruz, Mexico. In particular, we (1) compared the early decomposition of two litter types between two contrasting microsites in the canopy (branches and tank bromeliads) and terrestrial soil in five different forests, and (2) evaluated the effect of litter composition, microsite and climatic conditions (temperature and precipitation) on the initial phase of decomposition. We hypothesized that for both tea types: (1) decomposition differs among microsites and sites, (2) decomposition in the canopy is slower in dry microsites (branches) compared to wetter microsites (bromeliads) and soil, and (3) the effects of climatic variables on decomposition at regional scale differ among microsites.
Materials and methods
Study area
The present study was conducted in the central part of Veracruz, Mexico, along an elevational gradient formed by the eastern and southeastern slopes of the extinct volcano Cofre de Perote. Along this gradient, elevation ranges from sea level to 4250 m above the sea level across a geographical distance of less than 130 km (Fig. 1). The geomorphology is characterized by mountains, hills, and low plains of Cenozoic volcanic rocks, Meso-Cenozoic sedimentary rocks, and lacustrine and marine sediments (CONABIO 2011). The main soil groups are Andosols in the upper part of the gradient; Phaeozems and Vertisols combined with Lithosols, Rendzinas, and Luvisols in the middle part; and Vertisols, Regosols and Gleysols in the lower part (CONABIO and INIFAP 1995). Mean annual temperature (MAT) decreases linearly with elevation and mean annual precipitation (MAP) peaks at 1500–2000 m a.s.l. (Fig. 2). Remarkably, nearly all the climate types of Mexico are found along this gradient (CONABIO 2011). Varied climatic conditions and the complex topography maintain highly diverse plant communities, which have been classified into more than 27 elevational vegetation zones (Cházaro-Basáñez 1992). In this area, five study sites were selected at elevations of ca. 0, 600, 1000, 1600, and 2200 m a.s.l. corresponding with mangrove forest (Mandinga), semi-deciduous tropical forest (Palmarejo), tropical oak forest (Tres Pasos), and two different cloud forests (Capulines and El Zapotal), respectively (Table 1, Fig. 1).
Tea material
The decomposition experiment was carried out using the standard substrates proposed by Keuskamp et al. (2013), which consist of tea bags (Lipton, Unilever, Rotterdam, Netherlands) containing ca. 2 g of green tea (Camellia sinensis, EAN: 87 22700 05552 5) or rooibos tea (Aspalathus linearis, EAN: 87 22700 18843 8). Tea bag are all tetrahedron-shaped (each side ca. 5 cm) and made of woven nylon with a mesh size of 0.25 mm, which permits the entry of bacteria, fungi, and meso- and microfauna (Verhoef and Brussaard 1990; Bradford et al. 2002; Keuskamp et al. 2013).
Tea bag experiment
At each study site along the elevational gradient, we established three 15 m × 15 m plots separated by at least 10 m (nested spatial design, 5 sites, 3 plots per site). Height (estimated with clinometer) and diameter at breast height (DBH) of all trees with a DBH ≥ 5 cm were determined, and each tree was labelled and identified. Then, in each plot, five large trees (DBH > 20 cm) were randomly chosen by assigning a number to all large trees and taking a random sample without replacement (n = 75). Near each plot, conspecifics of the previously selected trees were inspected for straight branches of ca. 7 cm in diameter (Ø). Once located, the branches were cut using a manual saw, avoiding damage to the trees as much as possible. The straight parts of the branches without imperfections such as knots or holes were chosen, and 20 cm long sections (Ø1 = 7.1 ± 0.5 cm, Ø2 = 7.2 ± 0.5 cm, means ± SD, n = 75) were cut and cleaned, removing all vascular and non-vascular epiphytes if present. Afterwards, a total of 75 medium- to large-sized tank bromeliads [maximum leaf length > 30 cm (average of the three longest leaves, LL)] without inflorescences, belonging to common species at each site (Table 1) were collected in the vicinity of the plots. All bromeliads were labeled, and the number and length (LL) of green leaves were recorded.
Subsequently, bromeliads and branch sections (hereinafter referred to as branches) were relocated on the selected trees at ca. 5 m above the ground (5.3 ± 0.3 m, mean ± SD, n = 75, supplementary material Fig. S1). For this purpose, bromeliads were randomly assigned in each forest to the experimental trees and relocated by fastening them to rigid polyvinyl chloride (PVC) rings. PVC rings were 5-cm high with a diameter of 2.5, 5.8, or 7.6 cm depending on the size of the bromeliads (Fig. S2). Branches of the same species as the selected trees were fixed using angle brackets, adjusting the angle to < 5° against the horizontal (2.5 ± 1.4°, mean ± SD, n = 75, supplementary material Fig. S2). The inclination and height of the PVC rings and branches were controlled using a laser measuring tool (Model GLM 80 professional, Bosch, Stuttgart, Germany).
Before incubation started, tea bags were oven-dried at 70 °C for 48 h, and the initial mass was recorded. Additionally, to estimate the initial amount of tea, 20 empty bags (10 green, 10 rooibos) were dried and weighed, and the average mass was subtracted from the initial mass of the tea bags. Afterwards, each bag was marked with a unique code on the white side of the label with a permanent marker. For our experiment we performed a factorial design with three factors (2 × 3 × 5, n = 450), tea type (green and rooibos), microsite (soil, branch and bromeliad), and site (0, 600, 1000, 1600, and 2200 m a.s.l). In November 2018, 75 pairs of tea bags (one green and one rooibos) were placed in the relocated tank bromeliads in the middle portion of the rosette and deep inside the leaf base. Additionally, 75 pairs were fixed onto the upper surface of the branches using fishing lines, leaving a gap of ca. 5 cm between the two tea bags. Seventy-five pairs were also placed in the forest floor below the bromeliads and branches. These bags were buried in the upper 5 cm of the topsoil layer, additionally, they were tied with fishing lines to flags facilitating later recovery (supplementary material Fig. S3).
Tea bags were retrieved from the bromeliads, branches, and soil in February 2019 after 90 days of incubation, transported to the laboratory in individual plastic bags, cleaned of soil and roots, and oven-dried for 48 h at 60 °C. After cleaning, the mass of the remaining tea (without bags) was recorded. A total of 26 tea bags were lost during the experiment or discarded because they were torn or had larger holes (11 on branches, 2 in bromeliads, and 13 in soil).
Statistical analysis
Relative importance of microsite and site on mass loss
Litter decomposition was calculated as a proportion of tea mass loss. Beta generalized linear mixed-effects models (GLMM) with a logit link function were performed to test for differences in litter decomposition among microsites and sites for each tea type using the glmmTMB package (Brooks et al. 2017). The beta regression is useful for modeling of data limited to the 0–1 interval and gives less biased estimates of the mean compared to transformation‐based approaches (Douma and Weedon 2019). Fixed factors for these models are microsite (categorical with three levels), site (categorical with five levels) and their interaction. For these models we used plot nested to site as a random effect to account the spatial dependence of observations. The significance for each variable was evaluated with the Wald chi-square test and if the interaction among microsite and site was significant, multiple comparisons between microsites within each site were tested applying Tukey post hoc contrast tests with the lsmeans package (Lenth 2016). Additionally, to quantify the difference sources of variation in the data we use a Beta GLMM with a logit link function model with microsite set as fixed factor and tea type (categorical with two levels) and site as random factors (supplementary material Table S1). Separate analysis for each tea type were performed to investigate whether site, microsite and individual tea bags accounted differently for the variation of each tea type (supplementary material Table S2). Different variance components of the mixed models were obtained using the insight package (Lüdecke et al. 2019).
Effects of litter composition, climate and microsite on mass loss
Since detailed climatic information was not available for the study sites and some of them share the same nearest climatic station, monthly average temperature (TEMP) and total precipitation (PREC) data for the incubation months (November-February) were obtained from the 1 km2 spatial resolution dataset from WorldClim (Fick and Hijmans 2017). These values represent the average for the years 1970–2000. To assess the congruency of the climatic data we compared the TEMP and PREC values against temperature and precipitation data for the period November-February from 31 climatic stations (average for the years1951 to 2010) located along the gradient. Climatic data obtained from climatic stations and WorldClim were highly correlated (TEMP: r = 0.98, p < 0.001; PREC: r = 0.97, p < 0.001, Pearson's product moment correlation).
To model litter decomposition as a function of the litter composition, climate and microsite a Beta GLMM with a logit link function was fitted. Fixed variables for the model are microsite, TEMP (continuous), PREC (continuous) and tea type. The interaction terms are TEMP × microsite, PREC × microsite and TEMP × PREC. We use plot nested to site as random effect to account the spatial dependence of observations. Afterwards, if the interaction among microsite and the climatic variables were significant, we tested the effect of each climatic variable and tea type on mass loss for each microsite (branch, bromeliad and soil) using individual Beta GLMMs. For these models we used a logit link function and plot nested to site set as random effect.
Although the bromeliads used in our experiment were all relatively large (LL > 30 cm), they still differed in size. Therefore, we tested for a possible effect of plant size on tea decomposition along the elevation gradient using a beta GLMM with a logit link function and plant size (LL, continuous) set as fixed covariate and tea type and site as random factors (supplementary material Table S3, Fig S4).
All statistical analyses were performed using R version 3.6.2 (R Core Team 2020). Simulated observations were obtained from the fitted models and plotted against the observed values, and residual plots of the models were visually inspected for deviations from assumptions (supplementary material Figs. S5-S10). Goodness of fit for the models were calculated based on marginal and conditional R2 (Nakagawa and Schielzeth 2013).
Results
Relative importance of microsite and site on mass loss
Along the elevational gradient, almost three times as much green tea mass (56 ± 12%, mean ± SD) than rooibos tea mass (20 ± 6%) was lost during the experiment. For both tea types there was a significant interaction between microsite and site on mass loss [green (χ2 = 162.9, df = 8, p < 0.001), rooibos (χ2 = 58.2, df = 8, p < 0.001), Fig. 3)]. Tukey post-hoc tests for both tea types indicated that among microsites, loss of tea mass was consistently smallest on branches along the elevation gradient. Decomposition on branches was higher at relatively wet cloud forests (Capulines and Zapotal) and lower in a dry forest (Palmarejo, Fig. 3a, b). Only for green tea in Zapotal cloud forest decomposition was not significantly different among branches, bromeliads and soil (Fig. 3a). In soil and bromeliads, tea mass loss was higher for the former at the mangrove site and for the latter in the mangrove and the relatively dry forest of Palmarejo. In wetter forests at the middle and upper part of the gradient (Tres Pasos, Capulines, and Zapotal), tea decomposition in bromeliads and soil did not differ significantly (Fig. 3a, b). The analysis of data variation showed that 83% of the variation in mass loss was related to tea type while 11% was related to microsite and 4% to the overall site characteristics (supplementary material Table S1). A separate analysis for each tea type reveled only small variation in the mass loss among sites (σ2 < 0.05), and tea bags (σ2 < 0.01) for green and rooibos tea (supplementary material Tables S2).
Effects of litter composition, climate and microsite on mass loss
Our model showed a significant effect of tea type on mass loss (χ2 = 3212.9, df = 1, p < 0.001), and a similar response of mass loss to climatic variables for both tea types. However, along the elevation gradient the effects of climatic variables on decomposition differed between microsites, as revealed by the significant interaction among microsite and TEMP (χ2 = 49.9, df = 2, p < 0.001, Fig. 4) and PREC (χ2 = 17.4, df = 2, p < 0.001, Fig. 4). Tea mass loss in soil was positively correlated with TEMP but not with PREC while on branches decomposition decreased with TEMP and increased with PREC (Table 3). In bromeliads decomposition varied less, increasing only slightly with TEMP and, in contrast to branches, decreasing slightly with PREC (Table 3). Along the elevation gradient decomposition tended to converge in the upper part as TEMP decreased and PREC increased, and the differences between microsites were 40–80% smaller than in the dry part of the gradient (Fig. 3).
Discussion
Litter composition is an important direct regulator of litter decomposition at the global scale (Enríquez et al. 1993; Zhang et al. 2008; Djukic et al. 2018). Based on incubations performed in the soil and canopy in different forests along an elevation gradient, we found that overall litter composition explained 83% of the variability of mass loss while 11% was related to microsite and 4% to the overall site characteristics. In a study using the same tea bags (green and rooibos) across nine biomes, Djukic et al. (2018) found a consistent pattern for soil in which about 65% of variability in initial litter decomposition at global scale was explained by litter type while 13% was related to biome.
As expected because of its much higher water-soluble fraction, green tea decomposed faster than rooibos tea in all microsites and forest types (Keuskamp et al. 2013). Water-soluble substances (reducing sugars, phenolics and soluble organic carbon), are easily leached from leaf litter during the first week of incubation accounting for more than 20% of dry mass loss during the early stage of decomposition (Nykvist 1963; Steinke et al. 1993). Accordingly, during this stage, leaching losses of green tea can exceed those of rooibos tea twofold, and this process may be more related to the mass loss than microbial mineralization (Djukic et al. 2018). Thus, the use of allochthonous and dried litter can increase leaching losses substantially and reduce the effect of mineralization of organic C during the first months of incubation by increasing the time to build a microbial community (Bärlocher 1991; Gessner 1991; Clein and Schimel 1994). Consequently, the use of fresh local litter, which already possess the microbial signature of the sites, may change the effect of leaching and microbial C mineralization on mass loss and may reduce the strong effect of litter composition that we found using tea bags. For example, Didion et al. (2016) found a difference of 4% in mass loss between the local litter (fast decomposition Fagus sylvatica vs. the recalcitrant Pinus nigra) buried into the soil at 5 cm depth in the first year of decomposition, compared to 27% between tea types, with rooibos tea losing much less mass than the recalcitrant local litter. Experiments in which both substrates are compared are still needed in order to corroborate the patterns found in this study and to have a better understanding of the importance of litter composition on decomposition in the canopy. Additionally, the results about decomposition in the soil should be interpreted with caution, since in our experiment tea bag were buried in the upper 5 cm of the topsoil layer which could increase decomposition by buffering temperature changes and moisture deficits in comparison to studies carried out on or above the soil surface where stronger microclimate fluctuations may limit microbial-driven litter decomposition (Parton and Logan 1981; Ashcroft and Gollan 2013; Lee et al. 2014; Liu et al. 2015).
In the five forests, mass loss was slower in the canopy than in the soil only when litterbags were placed on branches (Fig. 3a, b). A slower decomposition rate in the canopy has also been reported for tropical, subtropical and temperate forests, when litter bags were put on branches, trunk reiterations or limb junctions (Nadkarni and Matelson 1991; Lindo and Winchester 2007; Cardelús 2010; Li et al. 2014). The slower decay in these canopy microsites can be explained by their particular microclimatic conditions and interaction with decomposer communities. Frequent water shortage due to fast evaporative drying and sudden changes in temperature are common on branches (Nadkarni 1994; Bohlman et al. 1995; Zotz and Hietz 2001; Stuntz et al. 2002). These harsh conditions reduce leaching losses, the density and diversity of decomposers (Lindo and Winchester 2006, 2007) and affect the duration and intensity of their activity (Coûteaux et al. 1995; Manzoni et al. 2012; Wang et al. 2014).
Nonetheless, this pattern varied widely, and mass loss was as fast or even faster in the canopy when compared to the forest floor when tea bags were placed in the leaf rosettes of bromeliads (Fig. 3a, b). Epiphytes and litter-trapping plants affect temperature in the canopy, increase humidity (Ortega-Solís et al., 2017; Scheffers et al., 2014; Stuntz et al., 2002), and mitigate dispersal limitations of decomposers while increasing their density and diversity (Vance and Nadkarni 1990; Paoletti et al. 1991; Gora and Lucas 2019). Unlike branches, bromeliads represent humid habitats (Zotz et al. 2020) in which the density of fungi, bacteria, and invertebrate decomposers can be higher than in terrestrial soil (Paoletti et al. 1991; Pittl et al. 2010). Moreover, the capacity of bromeliads to store substantial amounts of water in the tanks (Zotz et al. 2020) can increase leaching losses. Due to these particular characteristics, tank bromeliads act as natural biodigesters that potentiate the decomposition process. Only the Mandinga mangrove was exceptional in this regard: decomposition in the soil was significantly higher than in bromeliads for green tea. We buried the tea bags during the wet season when floods are common (López-Portillo and Ezcurra 2002), which increases leaching losses and may provide more suitable conditions for microorganisms to utilize the substrate (Dick and Osunkoya 2000). Similarly, rapid losses of tea mass were observed in mangrove forests in the Mexican Pacific and Caribbean, where tea bags lost ca. 70% of their original mass after 3 months of incubation (Djukic et al. 2018).
Along the elevational gradient, the mass loss in the soil was positively correlated with TEMP but we did not find a significant effect of PREC. However, our results suggested that decomposition in the soil also increased with PREC, but the very different soil conditions in the mangrove forest could mask the effect of this variable. These results support the conclusions of other studies conducted at large scales in terrestrial ecosystems (Berg et al. 1993; Zhang et al. 2008). Interestingly, this pattern was not consistent across microsites. Several recent studies showed that regional heterogeneity in microsites is only poorly captured by site-mean climatic data, especially in the case of moisture, which may lead to a mismatch between local mechanisms and broad-scale patterns (Loescher et al. 2014; Bradford et al. 2017). In our study, we found that the effects of climatic variables on decomposition differed between microsites, as revealed the significant interaction between microsite and TEMP and PREC which may be related to different moisture conditions. Unlike in soil, decomposition on branches was positively correlated with PREC, but negatively with TEMP. Branches represent relatively dry microsites. Bark holds little water, even after rainfall, and most of this water (> 90%) is usually lost within 24 h (Callaway et al. 2002; Einzmann et al. 2015). Branches are generally more exposed to sunlight than soil, so dry periods typically prevail and are only punctuated by pulses of rainfall (Benzing 1987; Zotz 2016). Thus, as in other relatively dry systems, abiotic factors may dominantly influence mass loss in these microsites, and precipitation and solar radiation could be the most important drivers that promote decomposition (Santos et al. 1984; Coûteaux et al. 1995; Austin and Vivanco 2006; Yahdjian et al. 2006). We suggest that the negative correlation between decomposition and TEMP on branches is due to the fact that, in general, aridity increases with temperature along the gradient, which should reduce leaching losses and suppress microbial activity regardless of temperature (Davidson et al. 2006; Wang et al. 2014). However, water limitation diminishes in the upper part of the gradient, increasing decomposition on branches and reducing the decay gap between microsites (Fig. 3).
Quite different from branches, the water-filled tanks of bromeliads represent “wetlands” in the canopy (Martinson et al. 2010), in which mass loss slightly increased with TEMP and decreased with PREC. This pattern could be the result of the aerobic-anaerobic cycles in tank bromeliads resulting from the duration of periods with and without water in the tanks. In the lower part of the elevational gradient where < 50% of days are rainy (> 0.1 mm) and fog is uncommon, bromeliad tanks are probably frequently not filled with water (Zotz and Thomas 1999). After a few days without rain, bromeliad tanks can dry out and remain so until the next rain event (Zotz and Thomas 1999). Conversely, in an almost constantly filled tank, organic decomposition can lead to prevailing anoxic conditions (Martinson et al. 2010; Goffredi et al. 2011; Lehours et al. 2016) that are rarely interrupted by aerobic conditions during drier periods. Therefore, a low PREC and high TEMP can reduce the duration of flooded anoxic periods, promoting aerobic environments and increasing mass loss (Reddy and Patrick Jr 1975; Neckles and Neill 1994; Anderson and Smith 2002). However, in the upper part of the gradient where PREC increases and TEMP decreases, the number of rainy days and fog events is higher. Thus, bromeliad tanks in cloud forests may constantly hold water, prolonging anoxic conditions (Martinson et al. 2010; Goffredi et al. 2011; Lehours et al. 2016) and decreasing the rate of mass loss (Reddy and Patrick Jr 1975; Neckles and Neill 1994).
Conclusions
Our study showed that microsite (forest floor, branch surface and within tank bromeliads) interacts with climate (TEMP and PREC), which modifies the overall decomposition patterns in the canopy. Thus, it is premature to generalize that leaf litter decay is slower in the canopy than on the forest floor, especially in wet forest where precipitation and fog are common (e.g. cloud forest) and litter trapping plants are abundant and can increase litter decomposition. These findings are important for our understanding of the general carbon dynamics in the canopy, particularly in Neotropical forests. In such forests, arboreal soil can comprise half or more of the canopy’s total organic matter (Nadkarni 1984; Nadkarni et al. 2004; Díaz et al. 2010; Gómez-González et al. 2017), bromeliads are by far the most important epiphytes in terms of biomass and abundance (Benzing 2000; Zotz 2016), and litter decomposition in these plants has been linked to methane emissions to the atmosphere, which Martinson et al. (2010) estimate to be 1.2 Tg CH4 year for all Neotropical forest. However, our study only focuses on the initial phase of litter decomposition in the forest canopy. Long-term decomposition experiments comparing both tea bags and local litter are needed to understand the later stages of litter decay, the formation of arboreal soil and to corroborate the patterns found in this study.
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Acknowledgements
The authors acknowledge Karina Osorio Salomón (INECOL), Susanne Töller (University Oldenburg), and José Emilio Aguilar Galván for their help during the fieldwork. We are also grateful for Ika Djukic (Environment Agency Austria), Carolina Ruiz Fernández (UNAM), and Hugo López Rosas (INECOL) for generating and providing decomposition data for Mexican mangroves, and for Allison Marie Jermain for reviewing the English style. The Functional Ecology department (INECOL) provided laboratory space and equipment to carry out this research. Y. Aguilar-Cruz was funded by the Consejo Nacional de Ciencia y Tecnología (CONACyT 408928) the Deutscher Akademischer Austauschdienst (DAAD 91569882) and the Heinz Neumüller Stiftung.
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Aguilar-Cruz, Y., García-Franco, J.G. & Zotz, G. Microsites and early litter decomposition patterns in the soil and forest canopy at regional scale. Biogeochemistry 151, 15–30 (2020). https://doi.org/10.1007/s10533-020-00705-3
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DOI: https://doi.org/10.1007/s10533-020-00705-3