Abstract
Polycyclic aromatic hydrocarbons (PAHs), as persistent organic contaminants, are a major source of concern due to their toxic effect on ecosystems and human health. This study attempted to isolate halotolerant PAHs degrading bacteria from saline oil-contaminated soils. Among the isolates, strain KDI with the highest 16S rRNA gene sequence similarity to Labedella gwakjiensis was able to reduce surface tension (ST) from 65.42 to 26.60 mN m−1 and increase the emulsification index to 81.04%, as a result of significant biosurfactant production. Response Surface Methodology (RSM) analysis was applied to optimize the factors, i.e. PAHs concentration and NaCl concentration as well as to determine the effect of these important variables on PAHs biodegradation. The Carbon Quantum Dots. Iron Oxide (CQDs.Fe3O4) nanoparticles were characterized by several popular analytical techniques, after which the effect of CQD.Fe3O4 nanoparticles on biodegradation was examined. PAHs biodegradation rate and efficiency of strain KDI to degrade PHE in the presence of CQD.Fe3O4 nanoparticles was analyzed by GC. According to the results during biodegradation both the concentration of PAHs and the amount of NaCl were effective. The biodegradation rate significantly increased in the presence of CQD.Fe3O4. The highest biodegradation of PHE occurred in the presence of 0.5 g/L of CQD.Fe3O4 which was 63.63% and 81.77% after 48 and 72 h of incubation. To the best of our knowledge, this is the first report on optimization of PAHs concentration and salinity by RSM and nanobioremediation of PHE using a bacterial strain in the presence of CQD.Fe3O4 nanoparticles.
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Authors are thankful to the Islamic Azad University, Science and Research Branch for providing the experimental equipment to carry out this research study.
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MF: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Visualization, Validation, Writing—original draft, Writing—review and editing. AAS: Conceptualization, Formal analysis, Project administration, Supervision, Validation, Writing—review and editing. HR: Validation, Writing—review and editing. FY: Conceptualization, Data curation, Formal analysis, Methodology, Validation, Visualization, Writing—review and editing.
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Firoozbakht, M., Sepahi, A.A., Rashedi, H. et al. Investigating the effect of nanoparticle on phenanthrene biodegradation by Labedella gwakjiensis strain KDI. Biodegradation 33, 441–460 (2022). https://doi.org/10.1007/s10532-022-09991-0
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DOI: https://doi.org/10.1007/s10532-022-09991-0