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Improved production of biosurfactant by a Pseudomonas aeruginosa mutant using vegetable oil refinery wastes

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Abstract

Biosurfactant production by Pseudomonas aeruginosa EBN-8 mutant was studied in shake flasks on separate wastes from canola, soybean and corn oil refineries. Of the substrates tested, canola oil refinery waste (COD=20 g l−1) supplemented with sodium nitrate (at COD/N=20) showed the best microbial growth (4.50 g l−1) and rhamnolipid production (8.50 g l−1), at 10 d of incubation with the specific growth rate of 0.316 h−1 and specific product yield of 0.597 g g−1 h. Its cell-free supernatant showed the critical micelle dilution (CMD) of 150 and surface tension (ST) of 28.5 mN m−1.

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Acknowledgements

The authors acknowledge the Higher Education Commission, Islamabad for funding for this research, Ch. Muhammad Akram, General Manager, Madni Ghee Mills, Faisalabad for providing the VORWs samples and Mr. Muhammad Afzal, Senior Scientist, NIBGE for his lab’s facilities.

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Authors and Affiliations

  1. Polymer Chemistry Laboratory, National Centre of Excellence in Physical Chemistry, University of Peshawar, 25210, Peshawar, Pakistan

    Zulfiqar Ali Raza & Muhammad Saleem Khan

  2. Environmental Biotechnology Division, National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Faisalabad, Pakistan

    Zulfiqar Ali Raza, Asma Rehman & Zafar M. Khalid

Authors
  1. Zulfiqar Ali Raza
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  2. Asma Rehman
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  3. Muhammad Saleem Khan
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  4. Zafar M. Khalid
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Correspondence to Zafar M. Khalid.

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Raza, Z.A., Rehman, A., Khan, M.S. et al. Improved production of biosurfactant by a Pseudomonas aeruginosa mutant using vegetable oil refinery wastes. Biodegradation 18, 115–121 (2007). https://doi.org/10.1007/s10532-006-9047-9

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  • DOI: https://doi.org/10.1007/s10532-006-9047-9

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