Abstract
S-Adenosylmethionine (SAM) is a crucial small-molecule metabolite widely used in food and medicine. The development of high-throughput biosensors for SAM biosynthesis can significantly improve the titer of SAM. This paper constructed a synthetic transcription factor (TF)-based biosensor for SAM detecting in Saccharomyces cerevisiae. The synthetic TF, named MetJ-hER-VP16, consists of an Escherichia coli-derived DNA-binding domain MetJ, GS linker, the human estrogen receptor binding domain hER, and the viral activation domain VP16. The synthetic biosensor is capable of sensing SAM in a dose-dependent manner with fluorescence as the output. Additionally, it is tightly regulated by the inducer SAM and β-estradiol, which means that the fluorescence output is only available when both are present together. The synthetic SAM biosensor could potentially be applied for high-throughput metabolic engineering and is expected to improve SAM production.
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Acknowledgements
This work was supported by the young backbone teachers funding project of Henan Province (2021GGJS047) the Students Research and Training Program (SRTP) of Henan University of Science and Technology (2022189).
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Chen, Y., Zheng, H., Yang, j. et al. Development of a synthetic transcription factor-based S-adenosylmethionine biosensor in Saccharomyces cerevisiae. Biotechnol Lett 45, 255–262 (2023). https://doi.org/10.1007/s10529-022-03338-8
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DOI: https://doi.org/10.1007/s10529-022-03338-8