Abstract
Objectives
Shellfish waste is a primary source for making N-acetyl-d-glucosamine. Thus, establishing a high-efficiency and low-cost bioconversion method to produce N-acetyl-d-glucosamine directly from shellfish waste was promising.
Results
A mutant C81 was obtained from Chitinolyticbacter meiyuanensis SYBC-H1 via 60Co-γ irradiation. This mutant C81 showed the highest chitinase activity of 9.8 U/mL that was 85% higher than the parent strain. The mutant C81 exhibted improved antioxidant activities, including total antioxidant capacity, superoxide radical ability, and hydroxyl radical scavenging ability, compared to that of the parent strain. Four out of nine organic solvents increased the chitinase activity by 1.9%, 6.8%, 11.7%, and 15.8%, corresponding to methylbenzene, n-heptane, petroleum ether, and n-hexane, respectively. The biphase system composed of aqueous and hexane presented a five-fold reduction of cell viability compared to the control. Using a continuous fermentation bioconversion process, 4.2 g/L GlcNAc was produced from crayfish shell powder with a yield of 80% of the chitin content.
Conclusions
This study demonstrated that the mutant C81 is suitable for converting crayfish shell powder into GlcNAc in an aqueous-organic system.
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Acknowledgements
This work was supported by the Science and Technology Project of Taizhou (1801gy24, 21gyb27), The Vertical Project Transmuted from the Horizontal Project of Taizhou Vocational and Technical college (2020HGZ01), the Doctoral Foundation Project of Taizhou Vocational and Technical College (2021BS02, 2016BSH01). Zhejiang Province Key Research and Development Plan (2021C03190), Scientific Research Foundation of Zhejiang A&F University (2034020081).
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Hao, Zk., Li, Js., Wang, Dh. et al. Efficient production of GlcNAc in an aqueous-organic system with a Chitinolyticbacter meiyuanensis SYBC-H1 mutant. Biotechnol Lett 44, 623–633 (2022). https://doi.org/10.1007/s10529-022-03248-9
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DOI: https://doi.org/10.1007/s10529-022-03248-9