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Hydrogel composite of lanthanum and Halorubrum ejinoor sp. cell lysate as an adsorbing material

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Abstract

Objectives

Although halophilic archaea are rich in natural environments, their biotechnological applications are not as prevalent as those of other extremophiles, such as thermophiles and alkaliphiles. This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp. (H.e.) which was isolated from a saline lake in Inner Mongolia, China. Furthermore, formation mechanism and potential applications of the hydrogel as an adsorbing material are discussed.

Results

Halorubrum ejinoor sp. (H.e.) cell lysate was firstly prepared by adding pure water onto the H.e. cell pellet, followed by a short incubation at 60 °C. The cell lysate was injected into different metal ion (or H+) solutions to obtain the hydrogel composite. It was observed that H+, Fe3+, La3+, Cu2+, and Ca2+ induced gelation of the cell lysate, while Fe2+, Co2+, Ni2+, Mg2+, Na+, and K+ did not. DNA and extracellular polysaccharides (EPS) in the H.e. cell lysate were found to be responsible for the gelation reaction. These results suggest that DNA and EPS should be crosslinked by metal ions (or H+) and form a networked structure in which the metal ion (or H+) serves as an anchor point. Potential application of the hydrogel as an adsorbing material was explored using La3+-induced H.e. hydrogel composite. The hydrogel composite can adsorb the fluoride, phosphate and DNA-binding carcinogenic agents, such as acridine orange.

Conclusions

The simplicity and cost effectiveness of the preparation method might make H.e. hydrogel a promising adsorbing material. This work is expected to expand the technical applications of haloarchaea.

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Acknowledgements

This work is supported by the Natural Science Foundation of Inner Mongolia, China (2018MS05001) and by National Natural Science Foundation of China (41661093).

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Authors and Affiliations

  1. College of Chemistry and Environmental Science, Inner Mongolia Normal University, Hohhot, 010020, China

    Gang Dai, Hao Wang,  Husile, Fenglan Zhou & Wenchao Liu

  2. Inner Mongolia Key Laboratory of Environmental Chemistry, Inner Mongolia Normal University, Hohhot, 010020, China

    Gang Dai

  3. College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, 028000, China

    Chaoluomeng

  4. Academic Support Center, Kogakuin University, Hachioji, Tokyo, 192-0015, Japan

    Tatsuo Iwasa

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  1. Gang Dai
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  2. Hao Wang
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Correspondence to Gang Dai.

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Dai, G., Wang, H., Husile et al. Hydrogel composite of lanthanum and Halorubrum ejinoor sp. cell lysate as an adsorbing material. Biotechnol Lett 43, 1443–1453 (2021). https://doi.org/10.1007/s10529-021-03132-y

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