Abstract
Objective
The effects of monosaccharide constituents of lignocellulosic materials on exopolysaccharide (EPS) production by Mesorhizobium sp. Semia 816 were studied.
Results
According to the results, by using sugars commonly found in lignocellulosic biomass as carbon sources (glucose, arabinose and xylose), no significant differences were observed in the production of EPS, reaching 3.39 g/L, 3.33 g/L and 3.27 g/L, respectively. Differences were observed in monosaccharide composition, mainly in relation to rhamnose and glucuronic acid contents (1.8 times higher when arabinose was compared with xylose). However, the biopolymers showed no differences in relation to rheological properties, with EPS aqueous-based suspensions (1.0% w/v) presenting pseudoplastic behavior, and a slight difference in degradation temperatures. Using soybean hulls hydrolysate as carbon source, slightly higher values were obtained (3.93 g/L).
Conclusion
The results indicate the potential of the use of lignocellulosic hydrolysates containing these sugars as a source of carbon in the cultivation of Mesorhizobium sp. Semia 816 for the production of EPS with potential industrial applications.
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Data availability
All data generated or analyzed during this study are included in this published article.
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Acknowledgements
The authors thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001 and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS).
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This study was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
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CAVB, EGML and TCLC contributed to the conception, design and supervision of the study. BCSR and RGV carried out the cultivation assays. BCSR and TCLC carried out the characterization of EPS samples and data analysis. All authors contributed to the first draft of the manuscript and manuscript revision.
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Roesler, B.C.S., Vaz, R.G., Castellane, T.C.L. et al. The potential of extracellular biopolymer production by Mesorhizobium sp. from monosaccharide constituents of lignocellulosic biomass. Biotechnol Lett 43, 1385–1394 (2021). https://doi.org/10.1007/s10529-021-03119-9
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DOI: https://doi.org/10.1007/s10529-021-03119-9