Abstract
Objectives
To enzymatically transform protopanaxatriol by using β-glucosidase from Thermotoga neapolitana (T. neapolitana) DSM 4359.
Results
Recombinant β-glucosidase was purified, which molecular weight was about 79.5 kDa. High levels of ginsenoside were obtained using the follow reaction conditions: 2 mg ml−1 ginsenoside, 25 U ml−1 enzyme, 85 °C, and pH 5.0. β-glucosidase converted ginsenoside Re to Rg2, Rf and Rg1 to APPT completely after 3 h under the given conditions, respectively. The enzyme created 1.66 mg ml−1 Rg2 from Re with 553 mg l−1 h−1, 0.85 mg ml−1, and 1.01 mg ml−1 APPT from Rg1 and Rf with 283 and 316 mg l−1 h−1 APPT.
Conclusions
β-glucosidase could be useful for the high-yield, rapid, and low-cost preparation of ginsenoside Rg2 from Re, and APPT from the ginsenosides Rg1 and Rf.
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Supporting information
Supplementary Table 1—Strains and plasmid used.
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This study was funded by Changbai Mountain Scholars Fund Project (Grant Number 00566).
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Bi, YF., Wang, XZ., Jiang, S. et al. Enzymatic transformation of ginsenosides Re, Rg1, and Rf to ginsenosides Rg2 and aglycon PPT by using β-glucosidase from Thermotoga neapolitana. Biotechnol Lett 41, 613–623 (2019). https://doi.org/10.1007/s10529-019-02665-7
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DOI: https://doi.org/10.1007/s10529-019-02665-7