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Combined effects of engineered tendon matrix and GDF-6 on bone marrow mesenchymal stem cell-based tendon regeneration

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Abstract

Objectives

To examine whether an engineered tendon matrix (ETM) environment and growth and differentiation factor-6 (GDF-6) have synergistic effects on the tenogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and the quality of tendon repair.

Results

ETM and GDF-6 promote tenogenic differentiation of BMSCs in vitro. Implantation of GDF-6-incorporated ETM containing BMSCs into a tendon injury model significantly improved the histological and mechanical properties of the repaired tendon.

Conclusions

GDF-6-incorporated ETM containing BMSCs represents a promising strategy for tendon injury repair.

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Acknowledgments

This work was supported by grants from the National Natural Sciences Foundation of China (No.30901516, 81472085).

Supporting information

Supplementary Table 1—Primer sequences of target genes for real-time RT-PCR.

Author information

Authors and Affiliations

  1. Department of Pediatric Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 KongJiang Road, Shanghai, 200092, China

    Dapeng Jiang & Yubo Zhang

  2. Department of Pediatric Surgery, Harbin Children Hospital, Harbin, 150086, China

    Peng Gao & Shulong Yang

Authors
  1. Dapeng Jiang
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  2. Peng Gao
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  3. Yubo Zhang
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  4. Shulong Yang
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Corresponding author

Correspondence to Dapeng Jiang.

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Jiang, D., Gao, P., Zhang, Y. et al. Combined effects of engineered tendon matrix and GDF-6 on bone marrow mesenchymal stem cell-based tendon regeneration. Biotechnol Lett 38, 885–892 (2016). https://doi.org/10.1007/s10529-016-2037-z

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  • DOI: https://doi.org/10.1007/s10529-016-2037-z

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