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Principles and application of antibody libraries for infectious diseases

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Abstract

Antibodies have been used efficiently for the treatment and diagnosis of many diseases. Recombinant antibody technology allows the generation of fully human antibodies. Phage display is the gold standard for the production of human antibodies in vitro. To generate monoclonal antibodies by phage display, the generation of antibody libraries is crucial. Antibody libraries are classified according to the source where the antibody gene sequences were obtained. The most useful library for infectious diseases is the immunized library. Immunized libraries would allow better and selective enrichment of antibodies against disease antigens. The antibodies generated from these libraries can be translated for both diagnostic and therapeutic applications. This review focuses on the generation of immunized antibody libraries and the potential applications of the antibodies derived from these libraries.

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Acknowledgments

The authors would like to acknowledge support by the Malaysian Ministry of Science, Technology and Innovation ScienceFund Grant (Grant No. 305/CIPPM/613229) and USM Short Term Research Grant (Grant No. 304/CIPPM/6312060).

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  1. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia

    Bee Nar Lim, Gee Jun Tye, Yee Siew Choong, Eugene Boon Beng Ong, Asma Ismail & Theam Soon Lim

  2. ADAPT Research Cluster, Centre for Research Initiatives - Clinical & Health Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia

    Theam Soon Lim

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Lim, B.N., Tye, G.J., Choong, Y.S. et al. Principles and application of antibody libraries for infectious diseases. Biotechnol Lett 36, 2381–2392 (2014). https://doi.org/10.1007/s10529-014-1635-x

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