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Cloning and starch degradation profile of maltotriose-producing amylases from Streptomyces species

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Abstract

The end products from starch hydrolysis by amylases have important applications in various industries. Here, two amylases derived from two Streptomyces species that hydrolyze soluble starch from potato produced maltotriose as the primary maltooligosaccharide product. The genes, annotated as putative glycoside hydrolases, were cloned and expressed in Streptomyces lividans. These amylases displayed hydrolysis activity from pH 3 to 9.5 and were not affected by Ca2+. Optimal production of maltotriose was between 20 and 30 °C at pH 6.5. At the optimal temperature, both amylases produced maltotriose-rich end products rather than either maltose or maltotetraose.

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Acknowledgments

This work was partially supported by Special Coordination Funds for Promoting Science and Technology, Creation of Innovation Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction, Kobe), Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 657-8501, Japan

    Norimasa Kashiwagi & Michiru Miyake

  2. R&D Center, Nagase & Co., Ltd., 2-2-3 Murotani, Nishi-ku, Kobe, 651-2241, Japan

    Shuichi Hirose & Masahiro Sota

  3. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 657-8501, Japan

    Chiaki Ogino & Akihiko Kondo

Authors
  1. Norimasa Kashiwagi
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  2. Michiru Miyake
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  3. Shuichi Hirose
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  4. Masahiro Sota
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  5. Chiaki Ogino
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  6. Akihiko Kondo
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Corresponding author

Correspondence to Akihiko Kondo.

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Kashiwagi, N., Miyake, M., Hirose, S. et al. Cloning and starch degradation profile of maltotriose-producing amylases from Streptomyces species. Biotechnol Lett 36, 2311–2317 (2014). https://doi.org/10.1007/s10529-014-1611-5

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  • DOI: https://doi.org/10.1007/s10529-014-1611-5

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