Abstract
l-Aspartyl l-amino acid methyl ester was synthesized using a mutant of a thermostable leucine aminopeptidase from Streptomyces cinnamoneus, D198 K SSAP, obtained in previously. A peptide of high-intensity sweetener, l-aspartyl-l-phenylalanine methyl ester, was selected as a model for demonstrating the synthesis of l-aspartyl l-amino acid methyl ester. The hydrolytic activities of D198 K SSAP toward l-aspartyl-l-phenylalanine and its methyl ester were, respectively, 74-fold and fourfold higher than those of wild type. Similarly, the initial rate of the enzyme for l-aspartyl-l-phenylalanine methyl ester synthesis was over fivefold higher than that of wild-type SSAP in 90% methanol (v/v) in a one-pot reaction. Furthermore, other l-aspartyl l-amino acid methyl esters were synthesized efficiently using D198 K SSAP. Results show that the substitution of Asp198 of SSAP with Lys is effective for synthesizing l-aspartyl l-amino acid methyl ester.
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This research was supported by a grant for young researchers from Nikki-Saneyoshi.
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Arima, J., Kono, M., Kita, M. et al. A mutant leucine aminopeptidase from Streptomyces cinnamoneus with enhanced l-aspartyl l-amino acid methyl ester synthetic activity. Biotechnol Lett 34, 1093–1099 (2012). https://doi.org/10.1007/s10529-012-0877-8
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DOI: https://doi.org/10.1007/s10529-012-0877-8