Abstract
A new superoxide dismutase (SOD) gene from the thermophilic fungus Chaetomium thermophilum (Ctsod) was cloned and expressed in Pichia pastoris and its gene product was characterized. The specific activity of the purified CtSOD was 2,170 U/mg protein. The enzyme was inactivated by KCN and H2O2 but not by NaN3, confirming that it belonged to the type of Cu, ZnSOD. The amino acid residues involved in coordinating copper and zinc were conserved. The recombinant CtSOD exhibited optimum activity at pH 6.5 and 60°C. The enzyme retained 65% of the maximum activity at 70°C for 60 min and the half-life was 22 and 7 min at 80 and 90°C, respectively. The recombinant yeast exhibited higher stress resistance than the control yeast cells to salt and superoxide-generating agents, such as paraquat and menadione.
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Acknowledgments
This work was supported by the Chinese National Nature Science Foundation (31071723), the Chinese National Programs for High Technology Research and Development (2006AA10Z304), and the Chinese Project of Transgenic Organisms (2008ZX08001-002). We thank Dr Jiarui Li from Kansas State University for his suggested revisions of the manuscript.
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Supplementary Fig. 1 Phylogenetic tree of Chaetomium thermophilum Cu, ZnSOD and other fungal Cu, ZnSODs. C.T: Chaetomium thermophilum (DQ493760); C.G: Chaetomium globosum (XP_001222495); K.M: Kluyveromyces marxianus (CAO02396); A.F: Aspergillus flavus (AAM94904); A.C: Aspergillus clavatus NRRL (XP_001274132); C.P: Coccidioides posadasii (ABF73315); N.H: Nectria haematococca mpVI (EEU33541); S.P: Schizosaccharomyces pombe (NP593163); C.L: Cryptococcus liquefaciens (BAF42028); P.M: Penicillium marneffei ATCC (XP_002148587). (TIFF 199 kb)
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Zhang, LQ., Guo, FX., Xian, HQ. et al. Expression of a novel thermostable Cu, Zn-superoxide dismutase from Chaetomium thermophilum in Pichia pastoris and its antioxidant properties. Biotechnol Lett 33, 1127–1132 (2011). https://doi.org/10.1007/s10529-011-0543-6
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DOI: https://doi.org/10.1007/s10529-011-0543-6