Abstract
Cellulose hydrolysis by immobilized Trichoderma reesei cellulase in the presence of a low viscosity ionic liquid, 1-ethyl-3-methylimidazolium diethyl phosphate (EMIM-DEP), was investigated. Preparation of the carrier-free immobilized cellulase was optimized with respect to concentration of the cross-linker and the type of precipitant. The addition of 2% (v/v) EMIM-DEP during hydrolysis gave an initial reaction rate 2.7 times higher than the hydrolysis rate with no ionic liquid. The initial yield after 2 h was 0.7 g glucose/g cellulose, and the carrier-free immobilized cellulase (CFIC) was effectively re-used five times.
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Jones, P.O., Vasudevan, P.T. Cellulose hydrolysis by immobilized Trichoderma reesei cellulase. Biotechnol Lett 32, 103–106 (2010). https://doi.org/10.1007/s10529-009-0119-x
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DOI: https://doi.org/10.1007/s10529-009-0119-x