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Involvement of G-alpha protein GNA3 in production of cell wall-degrading enzymes by Trichoderma reesei (Hypocrea jecorina) during mycoparasitism against Pythium ultimum

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Abstract

The involvement of the G-alpha protein GNA3 in the production of cell wall-degrading enzymes (CWDEs) by Trichoderma reesei during antagonism against Pythium ultimum was investigated. cAMP content was 2.8-fold higher in the T. reesei mutant gna3QL than in the parental TU-6. The gna3QL, like TU-6, inhibited the growth of P. ultimum in dual culture assays. Scanning electron microscopy showed that the gna3QL promoted more morphological alterations of P. ultimum cell wall than TU-6. In general, gna3QL produced higher activities of CWDEs than TU-6. We therefore suggest that CWDEs production during mycoparasitism by T. reesei against P. ultimum may be associated with the level of GNA3 activity.

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Acknowledgements

This work was supported by a biotechnology research grant to C.J.U. (CNPq and FUNAPE/UFG) and FINEP/CT-AGRO/Ação Transversal—01.07.0551.00. R.N·S was supported by CAPES/UNB. The authors thank Dr. Sônia Nair Baó (UnB/Cel) for her support with scanning electron microscopy.

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Correspondence to Cirano José Ulhoa.

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do Nascimento Silva, R., Steindorff, A.S., Ulhoa, C.J. et al. Involvement of G-alpha protein GNA3 in production of cell wall-degrading enzymes by Trichoderma reesei (Hypocrea jecorina) during mycoparasitism against Pythium ultimum . Biotechnol Lett 31, 531–536 (2009). https://doi.org/10.1007/s10529-008-9900-5

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  • DOI: https://doi.org/10.1007/s10529-008-9900-5

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