Abstract
Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1% of hINF-γ was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction.
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Acknowledgments
This work was partially supported by CONACyT Grant J39639-Q and a FOMIX grant FMSLP-2002–4100. E. Medina-Rivero thanks to CONACyT fellowship No. 157496. We thank Dr. Roderick Mackie (University of Illinois) for English corrections and Dr. A. De Las Peñas and Dr. I. Castaño (IPICyT) for critical reading of the manuscript.
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Medina-Rivero, E., Balderas-Hernández, V.E., Ordoñez-Acevedo, L.G. et al. Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli . Biotechnol Lett 29, 1369–1374 (2007). https://doi.org/10.1007/s10529-007-9395-5
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DOI: https://doi.org/10.1007/s10529-007-9395-5