Abstract
The putative raffinose synthase gene from rice was cloned and expressed in Escherichia coli. The enzyme displayed an optimum activity at 45°C and pH 7.0, and a sulfhydryl group was required for its activity. The enzyme was specific for galactinol and p-nitrophenyl-α-d-galactoside as galactosyl donors, and sucrose, lactose, 4−β-galactobiose, N-acetyl-d-lactosamine, trehalose and lacto-N-biose were recognized as galactosyl acceptors.
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Li, S., Li, T., Kim, WD. et al. Characterization of raffinose synthase from rice (Oryza sativa L. var. Nipponbare). Biotechnol Lett 29, 635–640 (2007). https://doi.org/10.1007/s10529-006-9268-3
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DOI: https://doi.org/10.1007/s10529-006-9268-3