Abstract
Protease-treated wheat bran (20% w/v) of particle size less than 300 µm containing 65% (w/w) starch was used for the simultaneous saccharification and l-(+)-lactic acid fermentation by the mixed cultures of Lactobacillus casei and Lactobacillus delbrueckii. Maximum lactate yield after various process optimizations was 123 gl−1 with a productivity of 2.3 gl−1 h−1 corresponding to a conversion of 0.95 g lactic acid per gram starch after 54 h at 37°C. By using protease-treated wheat bran around tenfold decrease in supplementation of the costly medium component, like yeast extract, was achieved together with a considerable increase in the production level.
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Acknowledgements
One of the authors (RPJ) is deeply indebted to Council of Scientific and Industrial Research (CSIR) for the award of Senior Research Fellowship for doctoral studies. The authors would like to thank CSIR Task force network programme on green technology (CMM 0006) for providing the financial support.
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John, R.P., Nampoothiri, K.M. & Pandey, A. Simultaneous saccharification and L-(+)-lactic acid fermentation of protease-treated wheat bran using mixed culture of lactobacilli. Biotechnol Lett 28, 1823–1826 (2006). https://doi.org/10.1007/s10529-006-9159-7
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DOI: https://doi.org/10.1007/s10529-006-9159-7