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Human Antimicrobial Peptides: Defensins, Cathelicidins and Histatins

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Abstract

Antimicrobial peptides, which have been isolated from many bacteria, fungi, plants, invertebrates and vertebrates, are an important component of the natural defenses of most living organisms. The isolated peptides are very heterogeneous in length, sequence and structure, but most of them are small, cationic and amphipathic. These peptides exhibit broad-spectrum activity against Gram-positive and Gram-negative bacteria, yeasts, fungi and enveloped viruses. A wide variety of human proteins and peptides also have antimicrobial activity and play important roles in innate immunity. In this review we discuss three important groups of human antimicrobial peptides. The defensins are cationic non-glycosylated peptides containing six cysteine residues that form three intramolecular disulfide bridges, resulting in a triple-stranded β-sheet structure. In humans, two classes of defensins can be found: α-defensins and β-defensins. The defensin-related HE2 isoforms will also be discussed. The second group is the family of histatins, which are small, cationic, histidine-rich peptides present in human saliva. Histatins adopt a random coil conformation in aqueous solvents and form α-helices in non-aqueous solvents. The third group comprises only one antimicrobial peptide, the cathelicidin LL−37. This peptide is derived proteolytically from the C-terminal end of the human CAP18 protein. Just like the histatins, it adopts a largely random coil conformation in a hydrophilic environment, and forms an α-helical structure in a hydrophobic environment.

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References

  • B Agerberth J Charo J Werr B Olsson F Idali L Lindbom R Kiessling H Jornvall H Wigzell GH Gudmundsson (2000) ArticleTitleThe human antimicrobial and chemotactic peptides LL−37 and alpha-defensins are expressed by specific lymphocyte and monocyte populations Blood 96 3086–3093 Occurrence Handle11049988

    PubMed  Google Scholar 

  • M Ahmad M Piludu FG Oppenheim EJ Helmerhorst AR Hand (2004) ArticleTitleImmunocytochemical localization of histatins in human salivary glands J. Histochem. Cytochem. 52 361–370 Occurrence Handle14966203

    PubMed  Google Scholar 

  • A Axen A Carlsson A Engstrom H Bennich (1997) ArticleTitleGloverin, an antibacterial protein from the immune hemolymph of Hyalophora pupae Eur. J. Biochem. 247 614–619 Occurrence Handle10.1111/j.1432-1033.1997.00614.x Occurrence Handle9266704

    Article  PubMed  Google Scholar 

  • D Baev XS Li J Dong P Keng M Edgerton (2002) ArticleTitleHuman salivary histatin 5 causes disordered volume regulation and cell cycle arrest in Candida albicans Infect. Immun. 70 4777–4784 Occurrence Handle10.1128/IAI.70.9.4777-4784.2002 Occurrence Handle12183519

    Article  PubMed  Google Scholar 

  • D Baev A Rivetta XS Li S Vylkova E Bashi CL Slayman M Edgerton (2003) ArticleTitleKilling of Candida albicans by human salivary histatin 5 is modulated, but not determined, by the potassium channel TOK1 Infect. Immun. 71 3251–3260 Occurrence Handle10.1128/IAI.71.6.3251-3260.2003 Occurrence Handle12761106

    Article  PubMed  Google Scholar 

  • D Baev A Rivetta S Vylkova JN Sun GF Zeng CL Slayman M Edgerton (2004) ArticleTitleThe TRK1 potassium transporter is the critical effector for killing of Candida albicans by the cationic protein, Histatin 5 J. Biol. Chem. 279 55060–55072 Occurrence Handle10.1074/jbc.M411031200 Occurrence Handle15485849

    Article  PubMed  Google Scholar 

  • R Bals (2000) ArticleTitleEpithelial antimicrobial peptides in host defense against infection Resp. Res. 1 141–150 Occurrence Handle10.1186/rr25

    Article  Google Scholar 

  • R Bals X Wang M Zasloff JM Wilson (1998) ArticleTitleThe peptide antibiotic LL−37/hCAP−18 is expressed in epithelia of the human lung where it has broad antimicrobial activity at the airway surface Proc. Natl. Acad. Sci. USA 95 9541–9546 Occurrence Handle10.1073/pnas.95.16.9541 Occurrence Handle9689116

    Article  PubMed  Google Scholar 

  • R Bals DJ Weiner AD Moscioni RL Meegalla JM Wilson (1999) ArticleTitleAugmentation of innate host defense by expression of a cathelicidin antimicrobial peptide Infect. Immun. 67 6084–6089 Occurrence Handle10531270

    PubMed  Google Scholar 

  • A Bateman A Singh LF Congote S Solomon (1991) ArticleTitleThe effect of HP−1 and related neutrophil granule peptides on DNA synthesis in HL60 cells Regul. Pept. 35 135–143 Occurrence Handle10.1016/0167-0115(91)90476-W Occurrence Handle1758971

    Article  PubMed  Google Scholar 

  • KW Bensch M Raida HJ Magert P Schulz-Knappe WG Forssmann (1995) ArticleTitlehBD-1: a novel beta-defensin from human plasma FEBS Lett. 368 331–335 Occurrence Handle10.1016/0014-5793(95)00687-5 Occurrence Handle7628632

    Article  PubMed  Google Scholar 

  • HG Boman B Agerberth A Boman (1993) ArticleTitleMechanisms of action on Escherichia coli of cecropin P1 and PR−39, two antibacterial peptides from pig intestine Infect. Immun. 61 2978–2984 Occurrence Handle8514403

    PubMed  Google Scholar 

  • M Brahmachary SP Krishnan JL Koh AM Khan SH Seah TW Tan V Brusic VB Bajic (2004) ArticleTitleANTIMIC: a database of antimicrobial sequences Nucleic Acids Res. 32 IssueIDDatabase issue D586–D589 Occurrence Handle10.1093/nar/gkh032 Occurrence Handle14681487

    Article  PubMed  Google Scholar 

  • M Castagnola R Inzitari DV Rossetti C Olmi T Cabras V Piras P Nicolussi MT Sanna M Pellegrini B Giardina I Messana (2004) ArticleTitleA cascade of 24 histatins (histatin 3 fragments) in human saliva. Suggestions for a pre-secretory sequential cleavage pathway J. Biol. Chem. 279 41436–41443 Occurrence Handle10.1074/jbc.M404322200 Occurrence Handle15272024

    Article  PubMed  Google Scholar 

  • YV Chaly EM Paleolog TS Kolesnikova II Tikhonov EV Petratchenko NN Voitenok (2000) ArticleTitleNeutrophil alpha-defensin human neutrophil peptide modulates cytokine production in human monocytes and adhesion molecule expression in endothelial cells Eur. Cytokine Netw. 11 257–266 Occurrence Handle10903805

    PubMed  Google Scholar 

  • PA Charp WG Rice RL Raynor E Reimund JM Kinkade T Ganz ME Selsted RI Lehrer JF Kuo (1988) ArticleTitleInhibition of protein kinase C by defensins, antibiotic peptides from human neutrophils Biochem. Pharmacol. 37 951–956 Occurrence Handle10.1016/0006-2952(88)90187-6 Occurrence Handle3345204

    Article  PubMed  Google Scholar 

  • SN Chitnis DB Kameswari KS Prasad (1993) ArticleTitleInduction of autolysis in starved Escherichia coli cells by seminalplasmin, an antimicrobial protein from bovine seminal plasma FEMS Microbiol. Lett. 112 147–150 Occurrence Handle10.1016/0378-1097(93)90156-V Occurrence Handle8405956

    Article  PubMed  Google Scholar 

  • JB Cowland AH Johnsen N Borregaard (1995) ArticleTitlehCAP−18, a cathelin/pro-bactenecin-like protein of human neutrophil specific granules FEBS Lett. 368 173–176 Occurrence Handle10.1016/0014-5793(95)00634-L Occurrence Handle7615076

    Article  PubMed  Google Scholar 

  • R Fernandez Caleya Particlede B Gonzalez-Pascual F Garcia-Olmedo P Carbonero (1972) ArticleTitleSusceptibility of phytopathogenic bacteria to wheat purothionins in vitro Appl. Microbiol. 23 998–1000 Occurrence Handle5031563

    PubMed  Google Scholar 

  • K Smet ParticleDe R Reekmans R Contreras (2004) ArticleTitleRole of oxidative phosphorylation in histatin 5-induced cell death in Saccharomyces cerevisiae Biotechnol. Lett. 26 1781–1785 Occurrence Handle10.1007/s10529-004-4608-7 Occurrence Handle15672214

    Article  PubMed  Google Scholar 

  • M Edgerton SE Koshlukova TE Lo BG Chrzan RM Straubinger PA Raj (1998) ArticleTitleCandidacidal activity of salivary histatins. Identification of a histatin 5-binding protein on Candida albicans J. Biol. Chem. 273 20438–20447 Occurrence Handle10.1074/jbc.273.32.20438 Occurrence Handle9685398

    Article  PubMed  Google Scholar 

  • P Engstrom A Carlsson A Engstrom ZJ Tao H Bennich (1984) ArticleTitleThe antibacterial effect of attacins from the silk moth Hyalophora cecropia is directed against the outer membrane of Escherichia coli Embo J. 3 3347–3351 Occurrence Handle6396089

    PubMed  Google Scholar 

  • M Frohm B Agerberth G Ahangari M Stahle-Backdahl S Liden H Wigzell GH Gudmundsson (1997) ArticleTitleThe expression of the gene coding for the antibacterial peptide LL−37 is induced in human keratinocytes during inflammatory disorders J. Biol. Chem. 272 15258–15263 Occurrence Handle10.1074/jbc.272.24.15258 Occurrence Handle9182550

    Article  PubMed  Google Scholar 

  • T Ganz ME Selsted RI Lehrer (1990) ArticleTitleDefensins Eur. J. Haematol. 44 1–8 Occurrence Handle2407547

    PubMed  Google Scholar 

  • T Ganz (2005) ArticleTitleDefensins and other antimicrobial peptides: a historical perspective and an update Comb. Chem. High Throughput Screen. 8 209–217 Occurrence Handle10.2174/1386207053764594 Occurrence Handle15892623

    Article  PubMed  Google Scholar 

  • JR Garcia A Krause S Schulz FJ Rodriguez-Jimenez E Kluver K Adermann U Forssmann A Frimpong-Boateng R Bals WG Forssmann (2001) ArticleTitleHuman beta-defensin 4: a novel inducible peptide with a specific salt-sensitive spectrum of antimicrobial activity FASEB J. 15 1819–1821 Occurrence Handle11481241

    PubMed  Google Scholar 

  • EA Groisman (1996) ArticleTitleBacterial responses to host-defense peptides Trends Microbiol 4 127–128. Occurrence Handle10.1016/0966-842X(96)30013-9 Occurrence Handle8728603

    Article  PubMed  Google Scholar 

  • GH Gudmundsson B Agerberth J Odeberg T Bergman B Olsson R Salcedo (1996) ArticleTitleThe human gene FALL39 and processing of the cathelin precursor to the antibacterial peptide LL−37 in␣granulocytes Eur. J. Biochem. 238 325–332 Occurrence Handle10.1111/j.1432-1033.1996.0325z.x Occurrence Handle8681941

    Article  PubMed  Google Scholar 

  • N Guex MC Peitsch (1997) ArticleTitleSWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling Electrophoresis 18 2714–2723 Occurrence Handle10.1002/elps.1150181505 Occurrence Handle9504803

    Article  PubMed  Google Scholar 

  • H Gusman J Travis EJ Helmerhorst J Potempa RF Troxler FG Oppenheim (2001a) ArticleTitleSalivary histatin 5 is an inhibitor of both host and bacterial enzymes implicated in periodontal disease Infect. Immun. 69 1402–1408 Occurrence Handle10.1128/IAI.69.3.1402-1408.2001

    Article  Google Scholar 

  • H Gusman U Lendenmann J Grogan RF Troxler FG Oppenheim (2001b) ArticleTitleIs salivary histatin 5 a metallopeptide? Biochim. Biophys. Acta 1545 86–95

    Google Scholar 

  • C Gyurko U Lendenmann RF Troxler FG Oppenheim (2000) ArticleTitleCandida albicans mutants deficient in respiration are resistant to the small cationic salivary antimicrobial peptide histatin 5 Antimicrob. Agents Chemother 44 348–354 Occurrence Handle10.1128/AAC.44.2.348-354.2000 Occurrence Handle10639361

    Article  PubMed  Google Scholar 

  • KG Hamil P Sivashanmugam RT Richardson G Grossman SM Ruben JL Mohler P Petrusz MG O’Rand FS French SH Hall (2000) ArticleTitleHE2beta and HE2gamma, new members of an epididymis-specific family of androgen-regulated proteins in the human Endocrinology 141 1245–1253 Occurrence Handle10.1210/en.141.3.1245 Occurrence Handle10698202

    Article  PubMed  Google Scholar 

  • RE Hancock G Diamond (2000) ArticleTitleThe role of cationic antimicrobial peptides in innate host defences Trends Microbiol. 8 402–410 Occurrence Handle10.1016/S0966-842X(00)01823-0 Occurrence Handle10989307

    Article  PubMed  Google Scholar 

  • J Harder J Bartels E Christophers JM Schroder (1997) ArticleTitleA peptide antibiotic from human skin Nature 387 861 Occurrence Handle10.1038/43088 Occurrence Handle9202117

    Article  PubMed  Google Scholar 

  • J Harder J Bartels E Christophers JM Schroder (2001) ArticleTitleIsolation and characterization of human beta-defensin−3, a novel human inducible peptide antibiotic J. Biol. Chem. 276 5707–5713 Occurrence Handle10.1074/jbc.M008557200 Occurrence Handle11085990

    Article  PubMed  Google Scholar 

  • EJ Helmerhorst IM Reijnders W van’t Hof I Simoons-Smit EC Veerman AV Amerongen (1999a) ArticleTitleAmphotericin B- and fluconazole-resistant Candida spp., Aspergillus fumigatus, and other newly emerging pathogenic fungi are susceptible to basic antifungal peptides Antimicrob. Agents Chemother 43 702–704

    Google Scholar 

  • EJ Helmerhorst P Breeuwer W van’t Hof E Walgreen-Weterings LC Oomen EC Veerman AV Amerongen T Abee (1999b) ArticleTitleThe cellular target of histatin 5 on Candida albicans is the energized mitochondrion J. Biol. Chem. 274 7286–7291 Occurrence Handle10.1074/jbc.274.11.7286

    Article  Google Scholar 

  • EJ Helmerhorst RF Troxler FG Oppenheim (2001) ArticleTitleThe human salivary peptide histatin 5 exerts its antifungal activity through the formation of reactive oxygen species Proc. Natl. Acad. Sci. USA 98 14637–14642 Occurrence Handle10.1073/pnas.141366998 Occurrence Handle11717389

    Article  PubMed  Google Scholar 

  • IB Holbrook PC Molan (1973) ArticleTitleA further study of the factors enhancing glycolysis in human saliva Arch. Oral Biol. 18 1275–1282 Occurrence Handle10.1016/0003-9969(73)90040-X Occurrence Handle4518747

    Article  PubMed  Google Scholar 

  • T Imatani T Kato K Minaguchi K Okuda (2000) ArticleTitleHistatin 5 inhibits inflammatory cytokine induction from human gingival fibroblasts by Porphyromonas gingivalis Oral Microbiol. Immunol. 15 378–382 Occurrence Handle10.1034/j.1399-302x.2000.150607.x Occurrence Handle11154435

    Article  PubMed  Google Scholar 

  • I Iontcheva FG Oppenheim RF Troxler (1997) ArticleTitleHuman salivary mucin MG1 selectively forms heterotypic complexes with amylase, proline-rich proteins, statherin, and histatins J. Dent. Res. 76 734–743 Occurrence Handle9109822

    PubMed  Google Scholar 

  • JL Jensen MS Lamkin FG Oppenheim (1992) ArticleTitleAdsorption of human salivary proteins to hydroxyapatite: a comparison between whole saliva and glandular salivary secretions J. Dent. Res. 71 1569–1576 Occurrence Handle1381733

    PubMed  Google Scholar 

  • S Joly C Maze PB McCray JM Guthmiller (2004) ArticleTitleHuman beta-defensins 2 and 3 demonstrate strain-selective activity against oral microorganisms J. Clin. Microbiol. 42 1024–1029 Occurrence Handle10.1128/JCM.42.3.1024-1029.2004 Occurrence Handle15004048

    Article  PubMed  Google Scholar 

  • DE Jones CL Bevins (1992) ArticleTitlePaneth cells of the human small intestine express an antimicrobial peptide gene J. Biol. Chem. 267 23216–23225 Occurrence Handle1429669

    PubMed  Google Scholar 

  • DE Jones CL Bevins (1993) ArticleTitleDefensin-6 mRNA in human Paneth cells: implications for antimicrobial peptides in host defense of the human bowel FEBS Lett. 315 187–192 Occurrence Handle10.1016/0014-5793(93)81160-2 Occurrence Handle8417977

    Article  PubMed  Google Scholar 

  • W Kamysz M Okroj J Lukasiak (2003) ArticleTitleNovel properties of antimicrobial peptides Acta Biochim. Pol. 50 461–469 Occurrence Handle12833170

    PubMed  Google Scholar 

  • K Kavanagh S Dowd (2004) ArticleTitleHistatins: antimicrobial peptides with therapeutic potential J. Pharm. Pharmacol. 56 285–289 Occurrence Handle10.1211/0022357022971 Occurrence Handle15025852

    Article  PubMed  Google Scholar 

  • SE Koshlukova MW Araujo D Baev M Edgerton (2000) ArticleTitleReleased ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans Infect. Immun. 68 6848–6856 Occurrence Handle10.1128/IAI.68.12.6848-6856.2000 Occurrence Handle11083804

    Article  PubMed  Google Scholar 

  • SE Koshlukova TL Lloyd MW Araujo M Edgerton (1999) ArticleTitleSalivary histatin 5 induces non-lytic release of ATP from Candida albicans leading to cell death J. Biol. Chem. 274 18872–18879 Occurrence Handle10.1074/jbc.274.27.18872 Occurrence Handle10383383

    Article  PubMed  Google Scholar 

  • JY Leem C Nishimura S Kurata I Shimada A Kobayashi S Natori (1996) ArticleTitlePurification and characterization of N-beta-alanyl−5-S-glutathionyl−3,4-dihydroxyphenylalanine, a novel antibacterial substance of Sarcophaga peregrina (flesh fly) J. Biol. Chem. 271 13573–13577 Occurrence Handle10.1074/jbc.271.23.13573 Occurrence Handle8662858

    Article  PubMed  Google Scholar 

  • RI Lehrer (2004) ArticleTitlePrimate defensins Nat. Rev. Microbiol. 2 727–738 Occurrence Handle10.1038/nrmicro976 Occurrence Handle15372083

    Article  PubMed  Google Scholar 

  • XS Li MS Reddy D Baev M Edgerton (2003) ArticleTitleCandida albicans Ssa1/2p is the cell envelope binding protein for human salivary histatin 5 J. Biol. Chem. 278 28553–28561 Occurrence Handle10.1074/jbc.M300680200 Occurrence Handle12761219

    Article  PubMed  Google Scholar 

  • A Lichtenstein T Ganz ME Selsted RI Lehrer (1986) ArticleTitleIn vitro tumor cell cytolysis mediated by peptide defensins of human and rabbit granulocytes Blood 68 1407–1410 Occurrence Handle3779104

    PubMed  Google Scholar 

  • BJ MacKay JJ Pollock VJ Iacono BJ Baum (1984a) ArticleTitleIsolation of milligram quantities of a group of histidine-rich polypeptides from human parotid saliva Infect. Immun. 44 688–694

    Google Scholar 

  • BJ MacKay L Denepitiya VJ Iacono SB Krost JJ Pollock (1984b) ArticleTitleGrowth-inhibitory and bactericidal effects of human parotid salivary histidine-rich polypeptides on Streptococcus mutans Infect. Immun. 44 695–701

    Google Scholar 

  • Y Murakami T Xu EJ Helmerhorst G Ori RF Troxler ET Lally FG Oppenheim (2002) ArticleTitleInhibitory effect of synthetic histatin 5 on leukotoxin from Actinobacillus actinomycetemcomitans Oral Microbiol. Immunol. 17 143–149 Occurrence Handle10.1034/j.1399-302X.2002.170302.x Occurrence Handle12030965

    Article  PubMed  Google Scholar 

  • CJ Murphy BA Foster MJ Mannis ME Selsted TW Reid (1993) ArticleTitleDefensins are mitogenic for epithelial cells and fibroblasts J. Cell. Physiol. 155 408–413 Occurrence Handle10.1002/jcp.1041550223 Occurrence Handle8482733

    Article  PubMed  Google Scholar 

  • K Okumura A Itoh E Isogai K Hirose Y Hosokawa Y Abiko T Shibata M Hirata H Isogai (2004) ArticleTitleC-terminal domain of human CAP18 antimicrobial peptide induces apoptosis in oral squamous cell carcinoma SAS-H1 cells Cancer Lett. 212 185–194 Occurrence Handle10.1016/j.canlet.2004.04.006 Occurrence Handle15279899

    Article  PubMed  Google Scholar 

  • FG Oppenheim T Xu FM McMillian SM Levitz RD Diamond GD Offner RF Troxler (1988) ArticleTitleHistatins, a novel family of histidine-rich proteins in human parotid secretion. Isolation, characterization, primary structure, and fungistatic effects on Candida albicans J. Biol. Chem. 263 7472–7477 Occurrence Handle3286634

    PubMed  Google Scholar 

  • JJ Oppenheim A Biragyn LW Kwak D Yang (2003) ArticleTitleRoles of antimicrobial peptides such as defensins in innate and adaptive immunity Ann. Rheum. Dis. 62 IssueIDSuppl 2 ii17–ii21 Occurrence Handle10.1136/ard.62.suppl_2.ii17 Occurrence Handle14532141

    Article  PubMed  Google Scholar 

  • C Osterhoff C Kirchhoff N Krull R Ivell (1994) ArticleTitleMolecular cloning and characterization of a novel human sperm antigen (HE2) specifically expressed in the proximal epididymis Biol. Reprod. 50 516–525 Occurrence Handle8167223

    PubMed  Google Scholar 

  • DW Paquette DM Simpson P Friden V Braman RC Williams (2002) ArticleTitleSafety and clinical effects of topical histatin gels in humans with experimental gingivitis J. Clin. Periodontol. 29 1051–1058 Occurrence Handle10.1034/j.1600-051X.2002.291201.x Occurrence Handle12492903

    Article  PubMed  Google Scholar 

  • CB Park HS Kim SC Kim (1998) ArticleTitleMechanism of action of the antimicrobial peptide buforin II: buforin II kills microorganisms by penetrating the cell membrane and inhibiting cellular functions Biochem. Biophys. Res. Commun. 244 253–257 Occurrence Handle10.1006/bbrc.1998.8159 Occurrence Handle9514864

    Article  PubMed  Google Scholar 

  • JJ Pollock L Denepitiya BJ MacKay VJ Iacono (1984) ArticleTitleFungistatic and fungicidal activity of human parotid salivary histidine-rich polypeptides on Candida albicans Infect. Immun. 44 702–707 Occurrence Handle6373615

    PubMed  Google Scholar 

  • PA Raj E Marcus DK Sukumaran (1998) ArticleTitleStructure of human salivary histatin 5 in aqueous and nonaqueous solutions Biopolymers 45 51–67 Occurrence Handle10.1002/(SICI)1097-0282(199801)45:1<51::AID-BIP5>3.0.CO;2-Y Occurrence Handle9433185

    Article  PubMed  Google Scholar 

  • KV Reddy RD Yedery C Aranha (2004) ArticleTitleAntimicrobial peptides: premises and promises Int. J. Antimicrob. Agents 24 536–547 Occurrence Handle10.1016/j.ijantimicag.2004.09.005 Occurrence Handle15555874

    Article  PubMed  Google Scholar 

  • DM Rothstein P Spacciapoli LT Tran T Xu FD Roberts M Dalla Serra DK Buxton FG Oppenheim P Friden (2001) ArticleTitleAnticandida activity is retained in P−113, a 12-amino-acid fragment of histatin 5 Antimicrob. Agents Chemother 45 1367–1373 Occurrence Handle10.1128/AAC.45.5.1367-1373.2001 Occurrence Handle11302797

    Article  PubMed  Google Scholar 

  • LM Sabatini EA Azen (1989) ArticleTitleHistatins, a family of salivary histidine-rich proteins, are encoded by at least two loci (HIS1 and HIS2) Biochem. Biophys. Res. Commun. 160 495–502 Occurrence Handle10.1016/0006-291X(89)92460-1 Occurrence Handle2719677

    Article  PubMed  Google Scholar 

  • US Sajjan LT Tran N Sole C Rovaldi A Akiyama PM Friden JF Forstner DM Rothstein (2001) ArticleTitleP−113D, an antimicrobial peptide active against Pseudomonas aeruginosa, retains activity in the presence of sputum from cystic fibrosis patients Antimicrob. Agents Chemother. 45 3437–3444 Occurrence Handle10.1128/AAC.45.12.3437-3444.2001 Occurrence Handle11709321

    Article  PubMed  Google Scholar 

  • BC Schutte JP Mitros JA Bartlett JD Walters HP Jia MJ Welsh TL Casavant PB McCray (2002) ArticleTitleDiscovery of five conserved beta-defensin gene clusters using a computational search strategy Proc. Natl. Acad. Sci. USA 99 2129–2133 Occurrence Handle10.1073/pnas.042692699 Occurrence Handle11854508

    Article  PubMed  Google Scholar 

  • OE Sorensen N Borregaard (2005) ArticleTitleCathelicidins–nature’s attempt at combinatorial chemistry Comb. Chem. High Throughput Screen 8 273–280 Occurrence Handle10.2174/1386207053764602 Occurrence Handle15892628

    Article  PubMed  Google Scholar 

  • MC Territo T Ganz ME Selsted R Lehrer (1989) ArticleTitleMonocyte-chemotactic activity of defensins from human neutrophils J. Clin. Invest. 84 2017–2020 Occurrence Handle2592571

    PubMed  Google Scholar 

  • H Tsai LA Bobek (1997) ArticleTitleHuman salivary histatin−5 exerts potent fungicidal activity against Cryptococcus neoformans Biochim. Biophys. Acta 1336 367–369 Occurrence Handle9367163

    PubMed  Google Scholar 

  • J Turner Y Cho NN Dinh AJ Waring RI Lehrer (1998) ArticleTitleActivities of LL−37, a cathelin-associated antimicrobial peptide of human neutrophils Antimicrob. Agents Chemother. 42 2206–2214 Occurrence Handle9736536

    PubMed  Google Scholar 

  • T Dyke ParticleVan D Paquette S Grossi V Braman J Massaro R D’Agostino S Dibart P Friden (2002) ArticleTitleClinical and microbial evaluation of a histatin-containing mouthrinse in humans with experimental gingivitis: a phase−2 multi-center study J. Clin. Periodontol. 29 168–176 Occurrence Handle10.1034/j.1600-051x.2002.290212.x Occurrence Handle11895545

    Article  PubMed  Google Scholar 

  • JC vanderSpek HE Wyandt JC Skare A Milunsky FG Oppenheim RF Troxler (1989) ArticleTitleLocalization of the genes for histatins to human chromosome 4q13 and tissue distribution of the mRNAs Am. J. Hum. Genet. 45 381–387 Occurrence Handle2773933

    PubMed  Google Scholar 

  • EC Veerman K Nazmi W Van’t Hof JG Bolscher AL Hertog ParticleDen AV Nieuw Amerongen (2004) ArticleTitleReactive oxygen species play no role in the candidacidal activity of the salivary antimicrobial peptide histatin 5 Biochem. J. 381 447–452 Occurrence Handle10.1042/BJ20040208 Occurrence Handle15109304

    Article  PubMed  Google Scholar 

  • M Velasco MJ Diaz-Guerra P Diaz-Achirica D Andreu L Rivas L Bosca (1997) ArticleTitleMacrophage triggering with cecropin A and melittin-derived peptides induces type II nitric oxide synthase expression J. Immunol. 158 4437–4443 Occurrence Handle9127009

    PubMed  Google Scholar 

  • HH Horsten Particlevon P Derr C Kirchhoff (2002) ArticleTitleNovel antimicrobial peptide of human epididymal duct origin Biol. Reprod 67 804–813 Occurrence Handle10.1095/biolreprod.102.004564 Occurrence Handle12193388

    Article  PubMed  Google Scholar 

  • Z Wang G Wang (2004) ArticleTitleAPD: the Antimicrobial Peptide Database Nucleic Acids Res. 32 IssueIDDatabase issue D590– D592 Occurrence Handle10.1093/nar/gkh025 Occurrence Handle14681488

    Article  PubMed  Google Scholar 

  • SH White WC Wimley ME Selsted (1995) ArticleTitleStructure, function, and membrane integration of defensins Curr. Opin. Struct. Biol. 5 521–527 Occurrence Handle10.1016/0959-440X(95)80038-7 Occurrence Handle8528769

    Article  PubMed  Google Scholar 

  • D Wunder J Dong D Baev M Edgerton (2004) ArticleTitleHuman salivary histatin 5 fungicidal action does not induce programmed cell death pathways in Candida albicans Antimicrob. Agents Chemother. 48 110–115 Occurrence Handle10.1128/AAC.48.1.110-115.2004 Occurrence Handle14693527

    Article  PubMed  Google Scholar 

  • Y Yamaguchi T Nagase R Makita S Fukuhara T Tomita T Tominaga H Kurihara Y Ouchi (2002) ArticleTitleIdentification of multiple novel epididymis-specific beta-defensin isoforms in humans and mice J. Immunol. 169 2516–2523 Occurrence Handle12193721

    PubMed  Google Scholar 

  • Q Yan A Bennick (1995) ArticleTitleIdentification of histatins as tannin-binding proteins in human saliva Biochem. J. 311 341–347 Occurrence Handle7575474

    PubMed  Google Scholar 

  • S Yenugu KG Hamil Y Radhakrishnan FS French SH Hall (2004a) ArticleTitleThe androgen-regulated epididymal sperm-binding protein, human beta-defensin 118 (DEFB118) (formerly ESC42), is an antimicrobial beta-defensin Endocrinology 145 3165–3173 Occurrence Handle10.1210/en.2003-1698

    Article  Google Scholar 

  • S Yenugu KG Hamil FS French SH Hall (2004b) ArticleTitleAntimicrobial actions of the human epididymis 2 (HE2) protein isoforms, HE2alpha, HE2beta1 and HE2beta2 Reprod. Biol. Endocrinol. 2 61 Occurrence Handle10.1186/1477-7827-2-61

    Article  Google Scholar 

  • YC Yoo R Watanabe Y Koike M Mitobe K Shimazaki S Watanabe I Azuma (1997) ArticleTitleApoptosis in human leukemic cells induced by lactoferricin, a bovine milk protein-derived peptide: involvement of reactive oxygen species Biochem. Biophys. Res. Commun. 237 624–628 Occurrence Handle10.1006/bbrc.1997.7199 Occurrence Handle9299415

    Article  PubMed  Google Scholar 

  • M Zanetti (2004) ArticleTitleCathelicidins, multifunctional peptides of the innate immunity J. Leukoc. Biol. 75 39–48 Occurrence Handle10.1189/jlb.0403147 Occurrence Handle12960280

    Article  PubMed  Google Scholar 

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  1. Kris De Smet

    Present address: Flen Pharma NV, Drie Eikenstraat 661, B−2650, Edegem, Belgium

Authors and Affiliations

  1. Unit of Fundamental and Applied Molecular Biology, Department for Molecular Biomedical Research, Ghent University and VIB, Technologiepark 927, B-9052, Ghent, Belgium

    Kris De Smet & Roland Contreras

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  1. Kris De Smet
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  2. Roland Contreras
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Correspondence to Roland Contreras.

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De Smet, K., Contreras, R. Human Antimicrobial Peptides: Defensins, Cathelicidins and Histatins. Biotechnol Lett 27, 1337–1347 (2005). https://doi.org/10.1007/s10529-005-0936-5

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  • DOI: https://doi.org/10.1007/s10529-005-0936-5

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