Abstract
The aim of this study was to investigate the role of circRNA insulin growth factor 1 receptor (circ-IGF1R) in colorectal cancer (CRC). Glycolytic metabolism was analyzed by glucose uptake and lactate production using the corresponding kits. The protein levels were determined using Western blot. The effect of circ-IGF1R on CRC in vivo was explored by xenograft experiment in mice. Circ-IGF1R was up-regulated in CRC tissues and cells. Knockdown of circ-IGF1R inhibited proliferation, migration, invasion and glycolysis but induced apoptosis of CRC cells. Circ-IGF1R interacted with miR-362-5p and miR-362-5p inhibitor attenuated the anti-tumor effects of circ-IGF1R downregulation on CRC cells. HMGB3 acted as a downstream target for miR-362-5p, and circ-IGF1R facilitated the malignant behaviors of CRC cells by regulating HMGB3. Circ-IGF1R activated the Wnt/β-catenin pathway via targeting miR-362-5p/HMGB3 axis. Tumor growth in vivo was reduced after knockdown of circ-IGF1R. Circ-IGF1R might be a novel biomolecular target for CRC diagnosis and treatment.
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Data Availability
The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Abbreviations
- CRC:
-
Colorectal cancer
- circRNAs:
-
Circular RNAs
- pre-mRNAs:
-
Precursor mRNAs
- circ-IGF1R:
-
Circular RNA insulin growth factor 1 receptor
- HCC:
-
Hepatocellular carcinoma
- miRNAs:
-
MicroRNAs
- TFR1:
-
Transferrin receptor 1
- HMGB3:
-
High mobility group-box 3
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XZ and WB: conceptualization and methodology. WB, JW and BJ: formal analysis and data curation. SG and XZ: validation and investigation. SG, XZ and WB: writing—original draft preparation and writing—review and editing. All authors: approval of final manuscript.
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Gao, S., Zhang, X., Bai, W. et al. Circ-IGF1R Affects the Progression of Colorectal Cancer by Activating the miR-362-5p/HMGB3-Mediated Wnt/β-Catenin Signal Pathway. Biochem Genet 61, 1210–1229 (2023). https://doi.org/10.1007/s10528-022-10316-2
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DOI: https://doi.org/10.1007/s10528-022-10316-2