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RETRACTED ARTICLE: Simple Sequence Repeat and S-locus Genotyping to Explore Genetic Variability in Polyploid Prunus spinosa and P. insititia

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This article was retracted on 23 June 2021

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Abstract

Polyploid Prunus spinosa (2n = 4×) and P. insititia (2n = 6×) represent enormous genetic potential in Central Europe, which can be exploited in breeding programmes. In Hungary, 17 cultivar candidates were selected from wild-growing populations including 10 P. spinosa, 4 P. insititia and three P. spinosa × P. domestica hybrids (2n = 5×). Their taxonomic classification was based on their phenotypic characteristics. Six simple sequence repeats (SSRs) and the multiallelic S-locus genotyping were used to characterize genetic variability and reliable identification of the tested accessions. A total of 98 SSR alleles were identified, which presents 19.5 average allele number per locus, and each of the 17 genotypes could be discriminated based on unique SSR fingerprints. A total of 23 S-RNase alleles were identified. The complete and partial S-genotype was determined for 8 and 9 accessions, respectively. The identification of a cross-incompatible pair of cultivar candidates and several semi-compatible combinations help maximize fruit set in commercial orchards. Our results indicate that the S-allele pools of wild-growing P. spinosa and P. insititia are overlapping in Hungary. A phylogenetic and principal component analysis confirmed the high level of diversity and genetic differentiation present within the analysed genotypes and helped clarify doubtful taxonomic identities. Our data confirm that S-locus genotyping is suitable for diversity studies in polyploid Prunus species. The analysed accessions represent huge genetic potential that can be exploited in commercial cultivation.

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Acknowledgments

This work was funded by the National Research, Development and Innovation Office (OTKA) K 112554 project. J. Halász is grateful for receiving the János Bolyai Research Scholarship of the Hungarian Academy of Sciences.

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Authors and Affiliations

  1. Department of Genetics and Plant Breeding, Szent István University, Ménesi út 44., Budapest, 1118, Hungary

    Júlia Halász, Noémi Makovics-Zsohár & Attila Hegedűs

  2. Petőfi u. 17., Lövőpetri, 4633, Hungary

    Ferenc Szőke

  3. Department of Horticulture, Faculty of Agriculture, Ataturk University, Erzurum, Turkey

    Sezai Ercisli

Authors
  1. Júlia Halász
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  2. Noémi Makovics-Zsohár
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  3. Ferenc Szőke
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  4. Sezai Ercisli
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  5. Attila Hegedűs
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Correspondence to Júlia Halász.

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Halász, J., Makovics-Zsohár, N., Szőke, F. et al. RETRACTED ARTICLE: Simple Sequence Repeat and S-locus Genotyping to Explore Genetic Variability in Polyploid Prunus spinosa and P. insititia. Biochem Genet 55, 22–33 (2017). https://doi.org/10.1007/s10528-016-9768-3

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  • DOI: https://doi.org/10.1007/s10528-016-9768-3

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