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Cloning of the Xuhuai Goat PPARγ Gene and the Preparation of Transgenic Sheep

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Abstract

This study aimed to clone the peroxisome proliferator-activated receptor γ (PPARγ) gene of the Xuhuai goat and to make transgenic sheep using intratesticular injection, so as to improve the meat quality and flavor by increasing the intramuscular fat content. The coding sequence of the goat PPARγ gene was 1,428 bp, encoding 475 amino acids. Its similarity with other species was 81 (chicken), 89 (mouse), 92 (pig), 98 (cow), and 99% (sheep). The similarity of the corresponding amino acid sequences was 92.9, 97.3, 98.3, 99.6, and 99.8%, respectively. The signal peptide region of the PPARγ protein was not found in this study, demonstrating that the protein is not secreted. RT-PCR and western blot revealed that PPARγ was expressed in vitro, and the protein was localized in the cytoplasm. The PPARγ gene was expressed in F1 transgenic sheep at both the mRNA and the protein levels; the positive ratio was 13.7%.

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Acknowledgments

The study was supported by National New Varieties of GMO Cultivation of Major Projects (2011ZX08008-003 and 2009ZX08008-003B) in China.

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Authors and Affiliations

  1. Academy of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China

    Yurong Qin, Yani Zhang, Caiye Zhu, Bo Gao, Yanhui Yin, Wei Li, Qingqing Shi, Mengmeng Zheng, Qi Xu & Bichun Li

  2. Medical School of Suzhou University, Suzhou, 215006, China

    Hao Chen

  3. Animal and Avian Science Department, University of Maryland, College Park, MD, 20742, USA

    Jiuzhou Song

Authors
  1. Yurong Qin
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  2. Hao Chen
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  3. Yani Zhang
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  4. Caiye Zhu
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  5. Bo Gao
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  6. Yanhui Yin
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  7. Wei Li
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  8. Qingqing Shi
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  9. Mengmeng Zheng
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  10. Qi Xu
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  11. Jiuzhou Song
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  12. Bichun Li
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Corresponding authors

Correspondence to Jiuzhou Song or Bichun Li.

Additional information

Yurong Qin and Hao Chen contributed equally to this study.

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Qin, Y., Chen, H., Zhang, Y. et al. Cloning of the Xuhuai Goat PPARγ Gene and the Preparation of Transgenic Sheep. Biochem Genet 51, 543–553 (2013). https://doi.org/10.1007/s10528-013-9585-x

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  • DOI: https://doi.org/10.1007/s10528-013-9585-x

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