Abstract
Gastrodia tuber and its component gastrodin have many pharmacological effects. The chemical fingerprints and gastrodin contents of eight Gastrodia populations were determined, and the genomic DNA polymorphism of the populations was investigated. Genetic distance coefficients among the populations were calculated using the DNA polymorphism data. A dendrogram of the genetic similarities between the populations was constructed using the genetic distance coefficients. The results indicated that the genomic DNA of Gastrodia tubers was highly polymorphic; the eight populations clustered into three major groups, and the gastrodin content varied greatly among these groups. There were obvious correlations among genetic makeup, gastrodin content, and place of origin. The ecological environments in Guizhou and Shanxi may be conducive to evolution and to gastrodin biosynthesis, and more suitable for cultivation of Gastrodia tubers. These findings may provide a scientific basis for overall genetic resource management and for the selection of locations for cultivating Gastrodia tubers.
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Acknowledgments
We extend our sincere thanks to Min Zhu, Jin-zhong Li, and De-hui Xiong for their support during this study. This study was financially supported by a key project grant (06A001) from the Hunan Provincial Educational Department and by the Hunan Provincial Natural Science Foundation of China (06JJ4036).
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MOESM1 Genomic DNA amplification products from eight Gastrodia tuber samples, using (A) primer 6; (B) primer 10; (C) primer 12; (D) primer 14; and (E) primer 15. (DOC 777 kb)
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Tao, J., Luo, Zy., Msangi, C.I. et al. Relationships Among Genetic Makeup, Active Ingredient Content, and Place of Origin of the Medicinal Plant Gastrodia Tuber. Biochem Genet 47, 8–18 (2009). https://doi.org/10.1007/s10528-008-9201-7
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DOI: https://doi.org/10.1007/s10528-008-9201-7