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Biocontrol of Pythium damping-off in cucumber by arbuscular mycorrhiza-associated bacteria from the genus Paenibacillus

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Abstract

The Pythium biocontrol features of 17 Paenibacillus strains, all previously isolated from the rhizosphere, hyphosphere or bulk soil from mycorrhizal and non-mycorrhizal cucumber plants, were examined using a cucumber seedling emergence bioassay. Thirteen strains – four strains of Paenibacillus polymyxa, eight strains of P. macerans and one strain of Paenibacillus sp. – significantly increased the percentage of seedling emergence of seeds inoculated with agar plugs of Pythium aphanidermatum FC42. Overall, the efficacy of Pythium biocontrol did not seem to differ between isolates of Paenibacillus originating from either mycorrhizal or non-mycorrhizal systems. No strains significantly reduced the damping-off incidence caused by the aggressive isolate Pythium sp. B5. Two strains of P. macerans not only reduced the incidence of pre-emergence damping-off by 73%, but they also counteracted the plant growth-depressing effect of P. aphanidermatum FC42, so that 68–82% of the emerged seedlings remained healthy 7 days after sowing. Two strains of P. macerans and one strain of P. polymyxa also significantly increased the percentage of seedling emergence following inoculation with approximately 105 zoospores of P. aphanidermatum FC42. There was no significant difference between the dry weight of three selected bacteria-inoculated and -uninoculated plants in the absence of Pythium; however, the dry weight of bacteria-inoculated plants was significantly higher than that of the uninoculated control plants with bacteria in the presence of P. aphanidermatum FC42.

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Acknowledgements

The work was supported by the Sino-Danish Scientific and Technological Cooperation New Project Proposal for 14th Session (2004–2006; Granted No. AM14: 33). Tina Tønnersen is thanked for excellent technical assistance. Dr. Gary D. Bending is thanked for providing us with Paenibacillus sp. EJ P73.

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Authors and Affiliations

  1. Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, China

    Bin Li & Guanlin Xie

  2. Institute of Integrated Pest Management, University of Aarhus, Research Centre Flakkebjerg, Slagelse, 4200, Denmark

    Bin Li, Sabine Ravnskov & John Larsen

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  1. Bin Li
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  2. Sabine Ravnskov
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  3. Guanlin Xie
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  4. John Larsen
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Correspondence to John Larsen.

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Li, B., Ravnskov, S., Xie, G. et al. Biocontrol of Pythium damping-off in cucumber by arbuscular mycorrhiza-associated bacteria from the genus Paenibacillus . BioControl 52, 863–875 (2007). https://doi.org/10.1007/s10526-007-9076-2

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  • DOI: https://doi.org/10.1007/s10526-007-9076-2

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