Abstract
The purpose of this work is to investigate the role of cellular stress-resistance mechanisms in the low-dose irradiation effects on Drosophila melanogaster lifespan. In males and females with the wild type Canton-S genotype the chronic low dose irradiation (40 cGy) induced the hormetic effect and radiation adaptive response to acute irradiation (30 Gy). The hormesis and radioadaptive responses were observed in flies with mutations in autophagy genes (atg7, atg8a) but absent in flies with mutations in FOXO, ATM, ATR, and p53 homologues. The hormetic effect was revealed in Sirt2 mutant males but not in females. On the contrary, the females but not males of JNK/+ mutant strain showed adaptive response. The obtained results demonstrate the essential role of FOXO, SIRT1, JNK, ATM, ATR, and p53 genes in hormesis and radiation adaptive response of the whole organism.
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Acknowledgments
This work was supported by the 08-04-00456-a grant from the Russian Foundation for Fundamental Research, by a grant from the Presidium of the Russian Academy of Science, and by a grant for Young Scientists from the Ural Division of the Russian Academy of Science. We are grateful to Ernst Hafen (Institute for Molecular Systems Biology, Zurich, Switzerland) and to the Drosophila Stock Center (Indiana University, Bloomington, Indiana, USA) for providing the Drosophila melanogaster strains. We are grateful to Maria Konovalenko for English language proof.
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Moskalev, A.A., Plyusnina, E.N. & Shaposhnikov, M.V. Radiation hormesis and radioadaptive response in Drosophila melanogaster flies with different genetic backgrounds: the role of cellular stress-resistance mechanisms. Biogerontology 12, 253–263 (2011). https://doi.org/10.1007/s10522-011-9320-0
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DOI: https://doi.org/10.1007/s10522-011-9320-0